ZFIN ID: ZDB-LAB-050803-1
Yoshihara Lab
PI/Director: Yoshihara, Yoshihiro
Contact Person: Yoshihara, Yoshihiro
Email: yoshihara@brain.riken.go.jp
URL: http://www.riken.jp/engn/r-world/research/lab/nokagaku/critical/synaps/index.html
Address: Laboratory for Neurobiology of Synapse RIKEN Brain Science Institute 2-1 Hirosawa, Wako-shi Saitama 351-0198, Japan
Country: Japan
Phone: +81-48-467-1699
Fax: +81-48-467-2306
Line Designation: rw

Show all 22 genomic features

The synapse is a specialized site of cell-to-cell contact, and is where information transfer between neurons takes place. The wiring patterns created by different types of neurons at specific synapses creates the functional logic that is the basis for information coding and processing in the brain. So, a detailed knowledge of the principles of neuronal networks is critical to understanding a wide variety of brain functions. In our laboratory, we have developed a powerful strategy for selectively visualizing neural pathways across synapses, one that combines traditional anatomical tracing and modern transgenic technology. We introduce cDNA from a plant lectin, wheat germ agglutinin (WGA), as a transgene into mice, but under the control of cell-type specific promoter elements in order to restrict its expression to particular types of neurons. With this WGA transgene technology, we are able to visualize functional neural circuits such as the olfactory, visual and cerebellar efferent pathways in these transgenic mice. With particular emphasis on the neuronal connections in the olfactory system, we are studying the cellular and molecular mechanisms that underlie the formation, maintenance, and remodeling of functional networks, and we are also seeking new concepts in neuronal connectivity patterns. In addition, we are also studying the structure, expression, and function of cell recognition and adhesion molecules at synapses in both the developing and the mature brain.

Miyasaka, Nobuhiko Research Staff Morimoto, Kozo Research Staff Sato, Yuki Research Staff

Masuda, M., Ihara, S., Mori, N., Koide, T., Miyasaka, N., Wakisaka, N., Yoshikawa, K., Watanabe, H., Touhara, K., Yoshihara, Y. (2024) Identification of olfactory alarm substances in zebrafish. Current biology : CB. 34(7):1377-1389.e7
Koide, T., Yabuki, Y., Yoshihara, Y. (2018) Terminal Nerve GnRH3 Neurons Mediate Slow Avoidance of Carbon Dioxide in Larval Zebrafish. Cell Reports. 22:1115-1123
Yoshihara, Y. (2017) [A sexy scent of prostaglandin F(2α): Identification of pheromone receptor mediating male courtship behavior in zebrafish]. Seikagaku. The Journal of Japanese Biochemical Society. 89:244-6
Yabuki, Y., Koide, T., Miyasaka, N., Wakisaka, N., Masuda, M., Ohkura, M., Nakai, J., Tsuge, K., Tsuchiya, S., Sugimoto, Y., Yoshihara, Y. (2016) Olfactory receptor for prostaglandin F2α mediates male fish courtship behavior. Nature Neuroscience. 19(7):897-904
Takeuchi, M., Matsuda, K., Yamaguchi, S., Asakawa, K., Miyasaka, N., Lal, P., Yoshihara, Y., Koga, A., Kawakami, K., Shimizu, T., Hibi, M. (2015) Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry. Developmental Biology. 397(1):1-17
Amo, R., Fredes, F., Kinoshita, M., Aoki, R., Aizawa, H., Agetsuma, M., Aoki, T., Shiraki, T., Kakinuma, H., Matsuda, M., Yamazaki, M., Takahoko, M., Tsuboi, T., Higashijima, S.I., Miyasaka, N., Koide, T., Yabuki, Y., Yoshihara, Y., Fukai, T., Okamoto, H. (2014) The Habenulo-Raphe Serotonergic Circuit Encodes an Aversive Expectation Value Essential for Adaptive Active Avoidance of Danger. Neuron. 84:1034-1048
Dozawa, M., Kono, H., Sato, Y., Ito, Y., Tanaka, H., Ohshima, T. (2014) Valproic acid, a histone deacetylase inhibitor, regulates cell proliferation in the adult zebrafish optic tectum. Developmental Dynamics : an official publication of the American Association of Anatomists. 243(11):1401-15
Nishiya, N., Oku, Y., Kumagai, Y., Sato, Y., Yamaguchi, E., Sasaki, A., Shoji, M., Ohnishi, Y., Okamoto, H., Uehara, Y. (2014) A Zebrafish Chemical Suppressor Screening Identifies Small Molecule Inhibitors of the Wnt/beta-catenin Pathway. Chemistry & Biology. 21:530-40
Miyasaka, N., Arganda-Carreras, I., Wakisaka, N., Masuda, M., Sümbül, U., Seung, H.S., Yoshihara, Y. (2014) Olfactory projectome in the zebrafish forebrain revealed by genetic single-neuron labelling. Nature communications. 5:3639
Miyasaka, N., Wanner, A.A., Li, J., Mack-Bucher, J., Genoud, C., Yoshihara, Y., and Friedrich, R.W. (2013) Functional development of the olfactory system in zebrafish. Mechanisms of Development. 130(6-8):336-46
Braubach, O.R., Miyasaka, N., Koide, T., Yoshihara, Y., Croll, R.P., and Fine, A. (2013) Experience-Dependent versus Experience-Independent Postembryonic Development of Distinct Groups of Zebrafish Olfactory Glomeruli. The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(16):6905-6916
Langhauser, M., Ustinova, J., Rivera-Milla, E., Ivannikov, D., Seidl, C., Slomka, C., Finne, J., Yoshihara, Y., Bastmeyer, M., and Bentrop, J. (2012) Ncam1a and Ncam1b: Two carriers of polysialic acid with different functions in the developing zebrafish nervous system. Glycobiology. 22(2):196-209
Koide, T., Miyasaka, N., Morimoto, K., Asakawa, K., Urasaki, A., Kawakami, K., and Yoshihara, Y. (2009) Olfactory neural circuitry for attraction to amino acids revealed by transposon-mediated gene trap approach in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 106(24):9884-9889
Miyasaka, N., Morimoto, K., Tsubokawa, T., Higashijima, S., Okamoto, H., and Yoshihara, Y. (2009) From the olfactory bulb to higher brain centers: genetic visualization of secondary olfactory pathways in zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 29(15):4756-4767
Yoshihara, Y. (2009) Molecular Genetic Dissection of the Zebrafish Olfactory System. Results and problems in cell differentiation. 47:1-24
Miyasaka, N., Knaut, H., and Yoshihara, Y. (2007) Cxcl12/Cxcr4 chemokine signaling is required for placode assembly and sensory axon pathfinding in the zebrafish olfactory system. Development (Cambridge, England). 134(13):2459-2468
Sato, Y., Miyasaka, N., and Yoshihara, Y. (2007) Hierarchical regulation of odorant receptor gene choice and subsequent axonal projection of olfactory sensory neurons in zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 27(7):1606-1615
Hirata, T., Nakazawa, M., Yoshihara, S., Miyachi, H., Kitamura, K., Yoshihara, Y., and Hibi, M. (2006) Zinc-finger gene Fez in the olfactory sensory neurons regulates development of the olfactory bulb non-cell-autonomously. Development (Cambridge, England). 133(8):1433-1443
Sato, Y., Miyasaka, N., and Yoshihara, Y. (2005) Mutually exclusive glomerular innervation by two distinct types of olfactory sensory neurons revealed in transgenic zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 25(20):4889-4897
Miyasaka, N., Sato, Y., Yeo, S.Y., Hutson, L.D., Chien, C.B., Okamoto, H., and Yoshihara, Y. (2005) Robo2 is required for establishment of a precise glomerular map in the zebrafish olfactory system. Development (Cambridge, England). 132(6):1283-1293
Miyasaka, N., Sato, Y., and Yoshihara, Y. (2005) Axon guidance of olfactory sensory neurons in zebrafish. Chemical senses. (Suppl.) 1:i92-i93
Mizuno, T., Kawasaki, M., Nakahira, M., Kagamiyama, H., Kikuchi, Y., Okamoto, H., Mori, K., and Yoshihara, Y. (2001) Molecular diversity in zebrafish NCAM family: three members with different VASE usage and distinct localization. Molecular and cellular neurosciences. 18(1):119-130