PUBLICATION
An atlas and database of neuropeptide gene expression in the adult zebrafish forebrain
- Authors
- Hiraki-Kajiyama, T., Miyasaka, N., Ando, R., Wakisaka, N., Itoga, H., Onami, S., Yoshihara, Y.
- ID
- ZDB-PUB-240604-12
- Date
- 2024
- Source
- The Journal of comparative neurology 532: e25619e25619 (Journal)
- Registered Authors
- Miyasaka, Nobuhiko, Yoshihara, Yoshihiro
- Keywords
- GABAergic and glutamatergic neuron marker, adult zebrafish brain (RR ID:NCBITaxon_7955), comparison with mouse, gene expression atlas, neuroanatomy, neuropeptide
- MeSH Terms
-
- Animals
- Atlases as Topic
- Databases, Genetic
- Gene Expression
- Mice
- Neuropeptides*/genetics
- Neuropeptides*/metabolism
- Prosencephalon*/metabolism
- Zebrafish*/anatomy & histology
- PubMed
- 38831653 Full text @ J. Comp. Neurol.
Citation
Hiraki-Kajiyama, T., Miyasaka, N., Ando, R., Wakisaka, N., Itoga, H., Onami, S., Yoshihara, Y. (2024) An atlas and database of neuropeptide gene expression in the adult zebrafish forebrain. The Journal of comparative neurology. 532:e25619e25619.
Abstract
Zebrafish is a useful model organism in neuroscience; however, its gene expression atlas in the adult brain is not well developed. In the present study, we examined the expression of 38 neuropeptides, comparing with GABAergic and glutamatergic neuron marker genes in the adult zebrafish brain by comprehensive in situ hybridization. The results are summarized as an expression atlas in 19 coronal planes of the forebrain. Furthermore, the scanned data of all brain sections were made publicly available in the Adult Zebrafish Brain Gene Expression Database (https://ssbd.riken.jp/azebex/). Based on these data, we performed detailed comparative neuroanatomical analyses of the hypothalamus and found that several regions previously described as one nucleus in the reference zebrafish brain atlas contain two or more subregions with significantly different neuropeptide/neurotransmitter expression profiles. Subsequently, we compared the expression data in zebrafish telencephalon and hypothalamus obtained in this study with those in mice, by performing a cluster analysis. As a result, several nuclei in zebrafish and mice were clustered in close vicinity. The present expression atlas, database, and anatomical findings will contribute to future neuroscience research using zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping