ZFIN ID: ZDB-TGCONSTRCT-190820-6
Transgenic Construct Name: Tg(myo6b:tmie-2A-NLS-mCherry)
Sequence Ontology ID :
Synonym: Tg(myo6b:tmie-p2A-NLS(mCherry)) (1)

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Note: In this construct, the myo6b promoter drives expression in sensory hair cells of WT tmie coupled to p2A-NLS(mCherry). The p2A linker is a self-cleaving peptide, which leads to translation of equimolar amounts of Tmie and NLS(mCherry). Hence, mCherry expression in the nucleus denotes Tmie expression in the cell.

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CONSTRUCT COMPONENTS
Regulatory Regions: myo6b (1)
Coding Sequences: mCherry (1)
2A (1), NLS (1)
tmie (1)
GENOMIC FEATURES THAT UTILIZE Tg(myo6b:tmie-2A-NLS-mCherry)
Genomic Feature Affected Genomic Regions    
zf2130Tg    
TRANSGENICS THAT UTILIZE Tg(myo6b:tmie-2A-NLS-mCherry)
Fish Affected Genomic Regions Phenotype Gene Expression
vo27Tg ; tmieru1000/ru1000 ; zf2130Tg(TU) tmie Fig. 4 with image from Pacentine et al., 2019
zf2130Tg(TU) Fig. 4 with image from Pacentine et al., 2019
zf2130Tg ; vo28Tg ; tmieru1000/ru1000(TU) tmie 2 figures with image from Pacentine et al., 2019
SEQUENCE INFORMATION No data available
OTHER Tg(myo6b:tmie-2A-NLS-mCherry) TRANSGENIC CONSTRUCT PAGES
CITATIONS (1)