Transgenic Construct

Tg6(Xla.Rho:crb2a-HA)

ID

ZDB-TGCONSTRCT-110131-42

Previous Names

Intra deltaFBDdelta PBD (1)
Tg(Xla.Rho:crb2.6-HA) (1)
Tg(Xla.Rho:crb2a.6-HA)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

crb2a (1)
HA (1)

Contains Sequences

HA (1)

Note

This construct utilizes 0.8 kB of the Xenopus rod opsin (Rho) promoter with an N terminal signal peptide takenfrom zebrafish Crb2b and an influenza hamagglutinin (HA) tag (YPYDVPDYA). To make the Intra deltaFBDdeltaPBD construct, site directed mutagenesis was performed on the Intra deltaPBD construct to introduce a Stop codon after E16.

Plasmid Map

None

Genomic Features

That Utilize Tg6(Xla.Rho:crb2a-HA) Construct

Genomic Feature	Affected Genomic Regions
umz19Tg
umz21Tg
umz22Tg

Transgenics

That Utilize Tg6(Xla.Rho:crb2a-HA) Construct

Fish	Affected Genomic Regions	Gene Expression
slc45a2^b4/+; fl1Tg/+; umz19Tg/+	slc45a2
slc45a2^b4/+; fl1Tg/+; umz21Tg/+	slc45a2
slc45a2^b4/b4; fl1Tg; umz19Tg	slc45a2	5 figures from Hsu et al., 2010
slc45a2^b4/b4; fl1Tg; umz21Tg	slc45a2
slc45a2^b4/b4; fl1Tg; umz22Tg	slc45a2
umz22Tg

Sequence Information

Other Construct Pages

None

Citations