ZFIN ID: ZDB-TGCONSTRCT-110131-39
Transgenic Construct Name: Tg4(Xla.Rho:crb2a-HA)
Sequence Ontology ID :
Synonyms: Intra deltaFBD (1), Tg(Xla.Rho:crb2.4-HA) (1), Tg(Xla.Rho:crb2a.4-HA)

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Note: This construct utilizes 0.8 kB of the Xenopus rod opsin (Rho) promoter with an N terminal signal peptide taken from zebrafish Crb2b and an influenza hamagglutinin (HA) tag (YPYDVPDYA). To make the Intra deltaFBD construct, the conserved amino acids tyrosine (T10) and glutamic acid (E16) of IntraWT construct were substituted with alanines to compromise the FERM-binding domain.

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CONSTRUCT COMPONENTS
Coding Sequences: HA (1)
crb2a (1)
Contains: HA (1)
GENOMIC FEATURES THAT UTILIZE Tg4(Xla.Rho:crb2a-HA)
Genomic Feature Affected Genomic Regions    
umz13Tg    
umz14Tg    
umz15Tg    
TRANSGENICS THAT UTILIZE Tg4(Xla.Rho:crb2a-HA)
Fish Affected Genomic Regions Phenotype Gene Expression
slc45a2b4/+; fl1Tg/+; umz13Tg/+ slc45a2
slc45a2b4/+; fl1Tg/+; umz14Tg/+ slc45a2
slc45a2b4/b4; fl1Tg; umz13Tg slc45a2 5 figures with image from Hsu et al., 2010
slc45a2b4/b4; fl1Tg; umz14Tg slc45a2
slc45a2b4/b4; fl1Tg; umz15Tg slc45a2
umz15Tg
SEQUENCE INFORMATIONNo data available
OTHER Tg4(Xla.Rho:crb2a-HA) TRANSGENIC CONSTRUCT PAGESNo links to external sites
CITATIONS (5)