Transgenic Construct
Tg3(Xla.Rho:crb2a-HA)
- ID
- ZDB-TGCONSTRCT-110131-38
- Name
- Tg3(Xla.Rho:crb2a-HA)
- Previous Names
- Type
- engineered_plasmid
- Regulatory Regions
- None
- Coding Sequences
- Contains Sequences
- Note
-
This construct utilizes 0.8 kB of the Xenopus rod opsin (Rho) promoter with an N terminal signal peptide takenfrom zebrafish Crb2b and an influenza hamagglutinin (HA) tag (YPYDVPDYA). To make IntraWt, the SP from zebrafish Crb2b was introduced with PCR using a pair of primers in which the HA tag sequence was engineered immediately after SP within the reverse primer and the sequence containing the Crb2a transmembrane domain plus the intracellular domain was cloned in-frame following the HA sequence.
Plasmid Map
None
Genomic Features
That Utilize Tg3(Xla.Rho:crb2a-HA) Construct
Transgenics
That Utilize Tg3(Xla.Rho:crb2a-HA) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| slc45a2b4/+; fl1Tg/+; umz10Tg/+ | |||
| slc45a2b4/+; fl1Tg/+; umz11Tg/+ | |||
| slc45a2b4/b4; fl1Tg; umz10Tg |
5 figures
from
Hsu et al., 2010
|
||
| slc45a2b4/b4; fl1Tg; umz11Tg | |||
| slc45a2b4/b4; fl1Tg; umz12Tg | |||
| umz12Tg |
|
Sequence Information
Other Construct Pages
None
Citations