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General Information
ZIRC
ZFIN ID: ZDB-TGCONSTRCT-110131-38
Transgenic Construct Name: Tg3(Xla.Rho:crb2a-HA)
Sequence Ontology ID :
Synonyms: IntraWT (1), Tg(Xla.Rho:crb2.3-HA) (1), Tg(Xla.Rho:crb2a.3-HA)

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Note: This construct utilizes 0.8 kB of the Xenopus rod opsin (Rho) promoter with an N terminal signal peptide takenfrom zebrafish Crb2b and an influenza hamagglutinin (HA) tag (YPYDVPDYA). To make IntraWt, the SP from zebrafish Crb2b was introduced with PCR using a pair of primers in which the HA tag sequence was engineered immediately after SP within the reverse primer and the sequence containing the Crb2a transmembrane domain plus the intracellular domain was cloned in-frame following the HA sequence.

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CONSTRUCT COMPONENTS
Coding Sequences: HA (1)
crb2a (1)
Contains: HA (1)
GENOMIC FEATURES THAT UTILIZE Tg3(Xla.Rho:crb2a-HA)
Genomic Feature Affected Genomic Regions    
umz10Tg    
umz11Tg    
umz12Tg    
SEQUENCE INFORMATIONNo data available
OTHER Tg3(Xla.Rho:crb2a-HA) TRANSGENIC CONSTRUCT PAGES
CITATIONS (5)