ZFIN ID: ZDB-TGCONSTRCT-110131-38
Transgenic Construct Name: Tg3(Xla.Rho:crb2a-HA)
Sequence Ontology ID :
Synonyms: IntraWT (1), Tg(Xla.Rho:crb2.3-HA) (1), Tg(Xla.Rho:crb2a.3-HA)

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Note: This construct utilizes 0.8 kB of the Xenopus rod opsin (Rho) promoter with an N terminal signal peptide takenfrom zebrafish Crb2b and an influenza hamagglutinin (HA) tag (YPYDVPDYA). To make IntraWt, the SP from zebrafish Crb2b was introduced with PCR using a pair of primers in which the HA tag sequence was engineered immediately after SP within the reverse primer and the sequence containing the Crb2a transmembrane domain plus the intracellular domain was cloned in-frame following the HA sequence.

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CONSTRUCT COMPONENTS
Coding Sequences: HA (1)
crb2a (1)
Contains: HA (1)
GENOMIC FEATURES THAT UTILIZE Tg3(Xla.Rho:crb2a-HA)
Genomic Feature Affected Genomic Regions    
umz10Tg    
umz11Tg    
umz12Tg    
TRANSGENICS THAT UTILIZE Tg3(Xla.Rho:crb2a-HA)
Fish Affected Genomic Regions Phenotype Gene Expression
slc45a2b4/+; fl1Tg/+; umz10Tg/+ slc45a2
slc45a2b4/+; fl1Tg/+; umz11Tg/+ slc45a2
slc45a2b4/b4; fl1Tg; umz10Tg slc45a2 5 figures with image from Hsu et al., 2010
slc45a2b4/b4; fl1Tg; umz11Tg slc45a2
slc45a2b4/b4; fl1Tg; umz12Tg slc45a2
umz12Tg
SEQUENCE INFORMATIONNo data available
OTHER Tg3(Xla.Rho:crb2a-HA) TRANSGENIC CONSTRUCT PAGESNo links to external sites
CITATIONS (5)