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ZFIN ID: ZDB-PUB-990628-13
Zebrafish genetic map with 2000 microsatellite markers
Shimoda, N., Knapik, E.W., Ziniti, J., Sim, C., Yamada, E., Kaplan, S., Jackson, D., de Sauvage, F., Jacob, H., and Fishman, M.C.
Date: 1999
Source: Genomics 58(3): 219-232 (Journal)
Registered Authors: de Sauvage, Frederic J., Fishman, Mark C., Jackson, Don, Knapik, Ela W., Shimoda, Nobuyoshi, Sim, Chäng, Ziniti, John
Keywords: zebrafish; linkage map; microsatellite repeat; polymorphism; microsatellite expansion
MeSH Terms:
  • Animals
  • Cloning, Molecular
  • Female
  • Gene Library
  • Genetic Linkage
  • Genome
  • Male
  • Microsatellite Repeats/genetics*
  • Mutation
  • Physical Chromosome Mapping
  • Zebrafish/genetics*
PubMed: 10373319 Full text @ Genomics
ABSTRACT
The zebrafish is the first vertebrate organism used for large-scale genetic screens seeking genes critical to development. These screens have been quite successful, with more than 1800 recessive mutations discovered that speak to morphogenesis of the vertebrate embryo. The cloning of the mutant genes depends on a dense genetic map. The 2000 markers we present here, using microsatellite (CA) repeats, provides 1.2-cM average resolution. One centimorgan in zebrafish is about 0.74 megabase, so, for many mutations, these markers are close enough to begin positional cloning by YAC walks.
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