ZFIN ID: ZDB-PERS-960805-181
Fishman, Mark C.
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Affiliation: The Fishman Laboratory
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BIOGRAPHY AND RESEARCH INTERESTS
***No longer involved in zebrafish research.***

The investigations of the Fishman laboratory are directed at understanding vertebrate morphogenesis. Genetics and embryology are used in combination to dissect organogenesis, especially of the cardiovasculature, gastrointestinal and hematopoietic systems.

Previous work has not utilized genetics in a systematic fashion to approach development of the heart or, for that matter, any organ system. The cornerstone of this project is the zebrafish, Danio rerio, which develops a functioning heart and vasculature within two days. We have identified a cardiogenic region of the blastula, and found unexpectedly early separation of progenitors for different chambers. Cell tracking and transplantation are being used to elucidate the embryological relationships between the myocardium, endocardium, vessels, and blood cells, and genes are being cloned which are useful in characterizing or perturbing these lineages. Cardiovascular mutants have been generated by several approaches, including radiation and ENU, the latter in collaboration with Wolfgang Driever. We have found that recessive cardiovascular mutations are reasonably frequent and can perturb unitary decisions, evidenced by discrete effects upon the heart and vasculature. For example, some mutants lack all heart valves, or are missing the entire endocardium, or maintain bilateral hearts. Other mutations provide models of human disease, such as porphyria, arrhythmias, electromechanical dissociation, and aortic coarctation. These mutants are under evaluation with regard to the underlying cell biology, their degree of cell autonomy, and for the mapping and cloning of the relevant genes. Similar types of mutational analyses are underway with regard to the gastrointestinal tract, pancreas, and liver.


PUBLICATIONS
Just, S., Hirth, S., Berger, I.M., Fishman, M.C., Rottbauer, W. (2016) The mediator complex subunit Med10 regulates heart valve formation in zebrafish by controlling Tbx2b-mediated Has2 expression and cardiac jelly formation. Biochemical and Biophysical Research Communications. 477(4):581-8
Just, S., Meder, B., Berger, I.M., Etard, C., Trano, N., Patzel, E., Hassel, D., Marquart, S., Dahme, T., Vogel, B., Fishman, M.C., Katus, H.A., Strähle, U., and Rottbauer, W. (2011) The myosin-interacting protein SMYD1 is essential for sarcomere organization. Journal of Cell Science. 124(Pt 18):3127-36
Peterson, R.T., and Fishman, M.C. (2011) Designing zebrafish chemical screens. Meth. Cell. Biol.. 105:525-541
Sogah, V.M., Serluca, F.C., Fishman, M.C., Yelon, D.L., MacRae, C.A., and Mably, J.D. (2010) Distinct troponin C isoform requirements in cardiac and skeletal muscle. Developmental dynamics : an official publication of the American Association of Anatomists. 239(11):3115-3123
Serluca, F.C., Xu, B., Okabe, N., Baker, K., Lin, S.Y., Sullivan-Brown, J., Konieczkowski, D.J., Jaffe, K.M., Bradner, J.M., Fishman, M.C., and Burdine, R.D. (2009) Mutations in zebrafish leucine-rich repeat-containing six-like affect cilia motility and result in pronephric cysts, but have variable effects on left-right patterning. Development (Cambridge, England). 136(10):1621-1631
Meder, B., Laufer, C., Hassel, D., Just, S., Marquart, S., Vogel, B., Hess, A., Fishman, M.C., Katus, H.A., and Rottbauer, W. (2009) A Single Serine in the Carboxyl Terminus of Cardiac Essential Myosin Light Chain-1 Controls Cardiomyocyte Contractility In Vivo. Circulation research. 104(5):650-659
Hassel, D., Scholz, E.P., Trano, N., Friedrich, O., Just, S., Meder, B., Weiss, D.L., Zitron, E., Marquart, S., Vogel, B., Karle, C.A., Seemann, G., Fishman, M.C., Katus, H.A., and Rottbauer, W. (2008) Deficient zebrafish ether-à-go-go-related gene channel gating causes short-QT syndrome in zebrafish reggae mutants. Circulation. 117(7):866-875
Bendig, G., Grimmler, M., Huttner, I.G., Wessels, G., Dahme, T., Just, S., Trano, N., Katus, H.A., Fishman, M.C., and Rottbauer, W. (2006) Integrin-linked kinase, a novel component of the cardiac mechanical stretch sensor, controls contractility in the zebrafish heart. Genes and Development. 20(17):2361-2372
Rottbauer, W., Wessels, G., Dahme, T., Just, S., Trano, N., Hassel, D., Burns, C.G., Katus, H.A., and Fishman, M.C. (2006) Cardiac Myosin Light Chain-2. A Novel Essential Component of Thick-Myofilament Assembly and Contractility of the Heart. Circulation research. 99(3):323-331
Mably, J.D., Chuang, L.P., Serluca, F.C., Mohideen, M.A., Chen, J.N., and Fishman, M.C. (2006) santa and valentine pattern concentric growth of cardiac myocardium in the zebrafish. Development (Cambridge, England). 133(16):3139-3146
Ebert, A.M., Hume, G.L., Warren, K.S., Cook, N.P., Burns, C.G., Mohideen, M.A., Siegal, G., Yelon, D., Fishman, M.C., and Garrity, D.M. (2005) Calcium extrusion is critical for cardiac morphogenesis and rhythm in embryonic zebrafish hearts. Proc. Natl. Acad. Sci. USA. 102(49):17705-17710
Burns, C.G., Milan, D.J., Grande, E.J., Rottbauer, W., MacRae, C.A., and Fishman, M.C. (2005) High-throughput assay for small molecules that modulate zebrafish embryonic heart rate. Nature Chemical Biology. 1(5):263-264
Rottbauer, W., Just, S., Wessels, G., Trano, N., Most, P., Katus, H.A., and Fishman, M.C. (2005) VEGF-PLC{gamma}1 pathway controls cardiac contractility in the embryonic heart. Genes and Development. 19(13):1624-1634
Torres-Vazquez, J., Gitler, A.D., Fraser, S.D., Berk, J.D., Pham, V.N., Fishman, M.C., Childs, S., Epstein, J.A., and Weinstein, B.M. (2004) Semaphorin-plexin signaling guides patterning of the developing vasculature. Developmental Cell. 7(1):117-123
Peterson, R.T., Shaw, S.Y., Peterson, T.A., Milan, D.J., Zhong, T.P., Schreiber, S.L., MacRae, C.A., and Fishman, M.C. (2004) Chemical suppression of a genetic mutation in a zebrafish model of aortic coarctation. Nat. Biotechnol.. 22(5):595-599
Peterson, R.T., and Fishman, M.C. (2004) Discovery and use of small molecules for probing biological processes in zebrafish. The Zebrafish: Cellular and Developmental Biology,2nd Ed. Methods Cell Biol.. 76:569-591
Mably, J.D., Mohideen, M.A.P.K., Burns, C.G., Chen, J.-N., and Fishman, M.C. (2003) heart of glass regulates the concentric growth of the heart in zebrafish. Current biology : CB. 13(24):2138-2147
Mayer, A.N. and Fishman, M.C. (2003) nil per os encodes a conserved RNA recognition motif protein required for morphogenesis and cytodifferentiation of digestive organs in zebrafish. Development (Cambridge, England). 130(17):3917-3928
Rottbauer, W., Saurin, A.J., Lickert, H., Shen, X., Burns, C.G., Wo, Z.G., Kemler, R., Kingston, R., Wu, C., and Fishman, M. (2002) Reptin and pontin antagonistically regulate heart growth in zebrafish embryos. Cell. 111(5):661-672
Ng, J.K., Kawakami, Y., Büscher, D., Raya, A., Itoh, T., Koth, C.M., Rodriguez-Esteban, C., Rodríguez-León, J., Garrity, D.M., Fishman, M.C., and Izpisúa Belmonte, J.C. (2002) The limb identity gene Tbx5 promotes limb initiation by interacting with Wnt2b and Fgf10. Development (Cambridge, England). 129(22):5161-5170
Garrity, D.M., Childs, S., and Fishman, M.C. (2002) The heartstrings mutation in zebrafish causes heart/fin Tbx5 deficiency syndrome. Development (Cambridge, England). 129(19):4635-4645
Handley, H., Grow, M., Fishman, M., and Stegeman, J. (2002) Generation of zebrafish cDNA microarrays for investigation of cardiovascular embryo toxicity by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Marine Environmental Research. 54(3-5):411-412
Roman, B.L., Pham, V., Lawson, N.D., Kulik, M., Childs, S., Lekven, A.C., Garrity, D.M., Moon, R.T., Fishman, M.C., Lechleider, R.J., and Weinstein, B.M. (2002) Disruption of acvrl1 increases endothelial cell number in zebrafish cranial vessels. Development (Cambridge, England). 129(12):3009-3019
Sehnert, A.J., Huq, A., Weinstein, B.M., Walker, C., Fishman, M., and Stainier, D.Y. (2002) Cardiac troponin T is essential in sarcomere assembly and cardiac contractility. Nature Genetics. 31(1):106-110
Mayer, A.N. and Fishman, M.C. (2002) npo encodes a novel RNA-binding protein essential for cytodifferentiation of digestive organs. Gastroenterology. 122(4):A95
Serluca, F.C., Drummond, I.A., and Fishman, M.C. (2002) Endothelial signaling in kidney morphogenesis. A role for hemodynamic forces. Current biology : CB. 12(6):492-497
Childs, S., Chen, J.-N., Garrity, D.M., and Fishman, M.C. (2002) Patterning of angiogenesis in the zebrafish embryo. Development (Cambridge, England). 129(4):973-982
Xu, X., Meiler, S.E., Zhong, T.P., Mohideen, M., Crossley, D.A., Burggren, W.W., and Fishman, M.C. (2002) Cardiomyopathy in zebrafish due to mutation in an alternatively spliced exon of titin. Nature Genetics. 30(2):205-209
Yelon, D., Weinstein, B.M., and Fishman, M.C. (2002) Cardiovascular system. Results Probl. Cell Diff.. 40:298-321
Shin, J.T. and Fishman, M.C. (2002) From zebrafish to human: modular medical models. Annual Review Genomics Human Genetics. 3:311-340
MacRae, C.A. and Fishman, M.C. (2002) Zebrafish: the complete cardiovascular compendium. Cold Spring Harbor symposia on quantitative biology. 67:301-307
Fishman, M.C. (2001) Genomics. Zebrafish--the canonical vertebrate. Science (New York, N.Y.). 294(5545):1290-1291
Zhong, T.P., Childs, S., Leu, J.P., and Fishman, M.C. (2001) Gridlock signalling pathway fashions the first embryonic artery. Nature. 414(6860):216-220
Peterson, R.T., Mably, J.D., Chen, J.-N., and Fishman, M.C. (2001) Convergence of distinct pathways to heart patterning reveals by the small molecule concentramide and the mutation heart-and-soul. Current biology : CB. 11:1481-1491
Serluca, F.C., Sidow, A., Mably, J.D., and Fishman, MC. (2001) Partitioning of tissue expression accompanies multiple duplications of the Na+/K+ ATPase alpha subunit gene. Genome research. 11(10):1625-1631
Warren, K.S., Baker, K., and Fishman, M.C. (2001) The slow mo mutation reduces pacemaker current and heart rate in adult zebrafish. Am. J. Physiol. Heart Circ. Physiol.. 281(4):H1711-H1719
Rottbauer, W., Baker, K., Wo, Z.G., Mohideen, M.A., Cantiello, H.F., and Fishman, M.C. (2001) Growth and function of the embryonic heart depend upon the cardiac-specific L-type calcium channel alpha1 subunit. Developmental Cell. 1(2):265-275
Chan, J., Mably, J.D., Serluca, F.C., Chen, J.-N., Goldstein, N.B., Thomas, M.C., Cleary, J.A., Brennan, C., Fishman, M.C., and Roberts, T.M. (2001) Morphogenesis of prechordal plate and notochord requires intact Eph/Ephrin B signaling. Developmental Biology. 234(2):470-482
Serluca, F.C. and Fishman, M.C. (2001) Pre-pattern in the pronephric kidney field of zebrafish. Development (Cambridge, England). 128(12):2233-2241
Garrity, D.M., Warren, K.S., Baker, K., Mohideen, M.A., and Fishman, M.C. (2001) The zebrafish mutant tremblor has a "fibrillating" heart. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 15(4):A481
Chen, J.-N., van Bebber, F., Goldstein, A.M., Serluca, F.C., Jackson, D., Childs, S., Serbedzija, G., Warren, K.S., Mably, J.D., Lindahl, P., Mayer, A., Haffter, P., and Fishman, M.C. (2001) Genetic steps to organ laterality in zebrafish. Comp. Func. Genomics. 2(2):60-68
Ton, C., Hwang, D.M., Dempsey, A.A., Tang, H.C., Yoon, J., Lim, M., Mably, J.D., Fishman, M.C., and Liew, C.C. (2000) Identification, characterization, and mapping of expressed sequence tags from an embryonic zebrafish heart cDNA library. Genome research. 10(12):1915-1927
Pasquinelli, A.E., Reinhart, B.J., Slack, F., Martindale, M.Q., Kuroda, M.I., Maller, B., Hayward, D.C., Ball, E.E., Degnan, B., Muller, P., Spring, J., Srinivasan, A., Fishman, M., Finnerty, J., Corbo, J., Levine, M., Leahy, P., Davidson, E., Ruvkun, G. (2000) Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA. Nature. 408(6808):86-89
Ton, C., Hwang, D.M., Dempsey, A.A., Cukeman, E., Francisco-Pabalan, O.L., Wei, W., Lee, S.Y., Mably, J.D., Fishman, M.C., and Liew, C.C. (2000) Identification, characterization and mapping of expressed sequence tags from zebrafish heart cDNA libraries. Circulation. 102(18):687
Chen, J.N. and Fishman, M.C. (2000) Genetics of heart development. Trends in genetics : TIG. 16(9):383-388
Childs, S., Weinstein, B.M., Mohideen, M.A.P.K., Donohue, S., Bonkovsky, H., and Fishman, M.C. (2000) Zebrafish dracula encodes ferrochelatase and its mutation provides a model for erythropoietic protoporphyria. Current biology : CB. 10(16):1001-1004
Warren, K.S., Wu, J.C., Pinet, F., and Fishman, M.C. (2000) The genetic basis of cardiac function: dissection by zebrafish (Danio rerio) screens. Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 355(1399):939-944
Zhong, T.P., Rosenberg, M., Mohideen, M.A.P.K., Weinstein, B., and Fishman, M.C. (2000) gridlock, an HLH gene required for assembly of the aorta in zebrafish. Science (New York, N.Y.). 287(5459):1820-1824
Chevrette, M., Joly, L., Tellis, P., Knapik, E.W., Miles, J., Fishman, M., and Ekker, M. (2000) Characterization of a zebrafish/mouse somatic cell hybrid panel. Genomics. 64(1):119-26
Fishman, M.C. (1999) Zebrafish genetics: the enigma of arrival. Proc. Natl. Acad. Sci. USA. 96(19):10554-10556
Shimoda, N., Knapik, E.W., Ziniti, J., Sim, C., Yamada, E., Kaplan, S., Jackson, D., de Sauvage, F., Jacob, H., and Fishman, M.C. (1999) Zebrafish genetic map with 2000 microsatellite markers. Genomics. 58(3):219-232
Amemiya, C.T., Zhong, T.P., Silverman, G.A., Fishman, M.C., and Zon, L.I. (1999) Zebrafish YAC, BAC, and PAC genomic libraries. In The Zebrafish: Genetics and Genomics, H.W. Detrich, III, M. Westerfield, and L.I. Zon, eds., San Diego, CA: Academic Press, Methods Cell Biol.. 60:235-258
Serluca, F.C. and Fishman, M.C. (1999) Cell lineage tracing in heart development. In The Zebrafish: Biology. H.W. Detrich, L.I. Zon, and M. Westerfield, eds. Methods Cell Biol.. 59:359-365
Drummond, I.A., Majumdar, A., Hentschel, H., Elger, M., Solnica-Krezel, L., Schier, A.F., Neuhauss, S.C., Stemple, D.L., Zwartkruis, F., Rangini, Z., Driever, W., and Fishman, M.C. (1998) Early development of the zebrafish pronephros and analysis of mutations affecting pronephric function. Development (Cambridge, England). 125:4655-4667
Goldstein, A.M. and Fishman, M.C. (1998) Notochord regulates cardiac lineage in zebrafish embryos. Developmental Biology. 201:247-252
Park, M., Lewis, C., Turbay, D., Chung, A., Chen, J.-N., Evans, S., Breitbart, R.E., Fishman, M.C., Izumo, S., and Bodmer, R. (1998) Differential rescue of visceral and cardiac defects in Drosophila by vertebrate tinman-related genes. Proc. Natl. Acad. Sci. USA. 95(16):9366-9371
Warren, K.S. and Fishman, M.C. (1998) Physiological genomics: mutant screens in zebrafish. The American journal of physiology. 275:H1-H7
Knapik, E.W., Goodman, A., Ekker, M., Chevrette, M., Delgado, J., Neuhauss, S., Shimoda, N., Driever, W., Fishman, M.C., and Jacob, H.J. (1998) A microsatellite genetic linkage map for zebrafish (Danio rerio). Nature Genetics. 18:338-343
Serbedzija, G.N., Chen, J.-N., and Fishman, M.C. (1998) Regulation in the heart field of zebrafish. Development (Cambridge, England). 125:1095-1101
Zhong, T.P., Kaphingst, K., Akella, U., Haldi, M., Lander, E.S., and Fishman, M.C. (1998) Zebrafish genomic library in yeast artificial chromosomes. Genomics. 48:136-138
Goldstein, A.M., Ticho, B.S., and Fishman, M.C. (1998) Patterning the heart's left-right axis: from zebrafish to man. Developmental genetics. 22:278-287
Chen, J.-N., van Eeden, F.J.M., Warren, K.S., Chin, A., Nüsslein-Volhard, C., Haffter, P., and Fishman, M.C. (1997) Left-right pattern of cardiac BMP4 may drive asymmetry of the heart in zebrafish. Development (Cambridge, England). 124(21):4373-4382
Fishman,M.C. and Chien, K.R. (1997) Fashioning the vertebrate heart: earliest embryonic decisions. Development (Cambridge, England). 124(11):2099-2117
Baker, K., Warren, K.S., Yellen, G., and Fishman, M.C. (1997) Defective "pacemaker" current (lh) in a zebrafish mutant with a slow heart rate. Proc. Natl. Acad. Sci. USA. 94(9):4554-4559
Fouquet, B., Weinstein, B.M., Serluca, F.C., and Fishman, M.C. (1997) Vessel patterning in the embryo of the zebrafish: guidance by notochord. Developmental Biology. 183(1):37-48
Fishman, M., D.Y. Stainier, R. Breitbart, and M. Westerfield (1997) Zebrafish: Genetic and embryological methods in a transparent vertebrate embryo. Meth. Cell. Biol.. 52:67-82
Knapik, E.W., Goodman, A., Atkinson, O.S., Roberts, C.T., Shiozawa, M., Sim, C.U., Weksler-Zagen, S., Trolliet, M.R., Futrell, C., Innes, B.A., Koike, G., McLaughlin, M., Pierre, L., Simon, J.S., Vilallonga, E., Roy, M., Chiang, P.W., Fishman, M.C., Driever, W., and Jacob, H.J. (1996) A reference cross DNA panel for zebrafish (Danio rerio) anchored with simple sequence length polymorphisms. Development (Cambridge, England). 123:451-460
Stainier, D.Y., Fouquet, B., Chen, J.N., Warren, K.S., Weinstein, B.M., Meiler, S.E., Mohideen, M.A., Neuhauss, S.C., Solnica-Krezel, L., Schier, A.F., Zwartkruis, F., Stemple, D.L., Malicki, J., Driever, W., and Fishman, M.C. (1996) Mutations affecting the formation and function of the cardiovascular system in the zebrafish embryo. Development (Cambridge, England). 123:285-292
Chen, J.N. and Fishman, M.C. (1996) Zebrafish tinman homolog demarcates the heart field and initiates myocardial differentiation. Development (Cambridge, England). 122(12):3809-3816
Weinstein, B.M., Schier, A.F., Abdelilah, S., Malicki, J., Solnica-Krezel, L., Stemple, D.L., Stainier, D.Y., Zwartkruis, F., Driever, W., and Fishman, M.C. (1996) Hematopoietic mutations in the zebrafish. Development (Cambridge, England). 123:303-309
Pack, M., Solnica-Krezel, L., Malicki, J., Neuhauss, S.C., Schier, A-, F., Stemple, D.L., Driever, W., and Fishman, M.C. (1996) Mutations affecting development of zebrafish digestive organs. Development (Cambridge, England). 123:321-328
Ticho, B.S., Stainier, D.Y.R., Fishman, M.C., and Breitbart, R.E. (1996) Three zebrafish MEF2 genes delineate somitic and cardiac muscle development in wild-type and mutant embryos. Mechanisms of Development. 59(2):205-218
Eder, P.S., Srinivasan, A., Fishman, M.C., and Altman, S. (1996) The RNA subunit of ribonuclease P from the zebrafish, Danio rerio. The Journal of biological chemistry. 271(35):21031-21036
Driever, W. and Fishman, M.C. (1996) The zebrafish: heritable disorders in transparent embryos. J. Clin. Invest.. 97(8):1788-94
Weinstein, B.M. and Fishman, M.C. (1996) Cardiovascular morphogenesis in zebrafish. Cardiovascular research. 31:E17-E24
Weinstein, B.M., Stemple, D.L., Driever, W., and Fishman, M.C. (1995) Gridlock, a localized heritable vascular patterning defect in the zebrafish. Nature medicine. 1(11):1143-1147
Stainier, D.Y.R., Weinstein, B.M., Detrich, H.W., III., Zon, L.I., and Fishman, M.C. (1995) cloche, an early acting zebrafish gene, is required by both the endothelial and hematopoietic lineages. Development (Cambridge, England). 121:3141-3150
Lee, R.K.K., Stainier, D.Y.R., Weinstein, B.M., and Fishman, M.C. (1994) Cardiovascular development in the zebrafish. II. Endocardial progenitors are sequestered within the heart field. Development (Cambridge, England). 120:3361-3366
Fishman, M.C. and Stainier, D.Y.R. (1994) Cardiovascular development: prospects for a genetic approach. Circulation research. 74:757-763
Stainier, D.Y.R. and Fishman, M.C. (1994) The zebrafish as a model system to study cardiovascular development. Trends in cardiovascular medicine. 4:207-212
Stainier, D.Y.R., Lee, R.K., and Fishman, M.C. (1993) Cardiovascular development in the zebrafish. I. Myocardial fate map and heart tube formation. Development (Cambridge, England). 119:31-40
Stainier, D.Y.R. and Fishman, M.C. (1993) Cardiac morphogenesis in the zebrafish, patterning the heart tube along the anteroposterior axis. In Molecular Basis of Morphogenesis, M. Bernfield, ed., New York: Wiley-Liss..
Stainier, D.Y.R. and Fishman, M.C. (1992) Patterning the zebrafish heart tube: acquisition of anteroposterior polarity. Developmental Biology. 153:91-101
Stainier, D.Y.R. and Fishman, M.C. (1992) Cardiovascular form and function: a genetic approach using the zebrafish embryo. Clin. Res.. 40:293

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