PUBLICATION
BF170 hydrochloride enhances the emergence of hematopoietic stem and progenitor cells
- Authors
- Liu, W., Ding, Y., Shen, Z., Xu, C., Yi, W., Wang, D., Zhou, Y., Zon, L.I., Liu, J.X.
- ID
- ZDB-PUB-240629-5
- Date
- 2024
- Source
- Development (Cambridge, England) 151(13): (Journal)
- Registered Authors
- Zhou, Yi, Zon, Leonard I.
- Keywords
- BF170 hydrochloride, Blood flow, Ca2+, HSPCs, Notch/NO, Primary cilia
- MeSH Terms
-
- Animals
- Blastomeres/cytology
- Blastomeres/drug effects
- Blastomeres/metabolism
- Cell Differentiation*/drug effects
- Cells, Cultured
- Cilia/drug effects
- Cilia/metabolism
- Embryoid Bodies/cytology
- Embryoid Bodies/drug effects
- Embryoid Bodies/metabolism
- Hematopoiesis*/drug effects
- Hematopoietic Stem Cells*/cytology
- Hematopoietic Stem Cells*/drug effects
- Hematopoietic Stem Cells*/metabolism
- Mice
- Receptors, Notch/metabolism
- Signal Transduction/drug effects
- Zebrafish*
- PubMed
- 38940293 Full text @ Development
Citation
Liu, W., Ding, Y., Shen, Z., Xu, C., Yi, W., Wang, D., Zhou, Y., Zon, L.I., Liu, J.X. (2024) BF170 hydrochloride enhances the emergence of hematopoietic stem and progenitor cells. Development (Cambridge, England). 151(13):.
Abstract
Generation of hematopoietic stem and progenitor cells (HSPCs) ex vivo and in vivo, especially the generation of safe therapeutic HSPCs, still remains inefficient. In this study, we have identified compound BF170 hydrochloride as a previously unreported pro-hematopoiesis molecule, using the differentiation assays of primary zebrafish blastomere cell culture and mouse embryoid bodies (EBs), and we demonstrate that BF170 hydrochloride promoted definitive hematopoiesis in vivo. During zebrafish definitive hematopoiesis, BF170 hydrochloride increases blood flow, expands hemogenic endothelium (HE) cells and promotes HSPC emergence. Mechanistically, the primary cilia-Ca2+-Notch/NO signaling pathway, which is downstream of the blood flow, mediated the effects of BF170 hydrochloride on HSPC induction in vivo. Our findings, for the first time, reveal that BF170 hydrochloride is a compound that enhances HSPC induction and may be applied to the ex vivo expansion of HSPCs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping