PUBLICATION
Zebrafish as a high throughput model for organ preservation and transplantation research
- Authors
- Da Silveira Cavalcante, L., Lopera Higuita, M., González-Rosa, J.M., Marques, B., To, S., Pendexter, C.A., Cronin, S.E.J., Gopinathan, K., de Vries, R.J., Ellett, F., Uygun, K., Langenau, D.M., Toner, M., Tessier, S.N.
- ID
- ZDB-PUB-231002-45
- Date
- 2023
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37: e23187e23187 (Journal)
- Registered Authors
- Ellett, Felix, Langenau, David
- Keywords
- cardiac graft, cryobiology, partial freezing, solid organ preservation, zebrafish
- MeSH Terms
-
- Animals
- Biological Assay
- Coculture Techniques
- Larva
- Mammals
- Organ Preservation*
- Zebrafish*
- PubMed
- 37718489 Full text @ FASEB J.
Citation
Da Silveira Cavalcante, L., Lopera Higuita, M., González-Rosa, J.M., Marques, B., To, S., Pendexter, C.A., Cronin, S.E.J., Gopinathan, K., de Vries, R.J., Ellett, F., Uygun, K., Langenau, D.M., Toner, M., Tessier, S.N. (2023) Zebrafish as a high throughput model for organ preservation and transplantation research. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 37:e23187e23187.
Abstract
Despite decades of effort, the preservation of complex organs for transplantation remains a significant barrier that exacerbates the organ shortage crisis. Progress in organ preservation research is significantly hindered by suboptimal research tools that force investigators to sacrifice translatability over throughput. For instance, simple model systems, such as single cell monolayers or co-cultures, lack native tissue structure and functional assessment, while mammalian whole organs are complex systems with confounding variables not compatible with high-throughput experimentation. In response, diverse fields and industries have bridged this experimental gap through the development of rich and robust resources for the use of zebrafish as a model organism. Through this study, we aim to demonstrate the value zebrafish pose for the fields of solid organ preservation and transplantation, especially with respect to experimental transplantation efforts. A wide array of methods were customized and validated for preservation-specific experimentation utilizing zebrafish, including the development of assays at multiple developmental stages (larvae and adult), methods for loading and unloading preservation agents, and the development of viability scores to quantify functional outcomes. Using this platform, the largest and most comprehensive screen of cryoprotectant agents (CPAs) was performed to determine their toxicity and efficiency at preserving complex organ systems using a high subzero approach called partial freezing (i.e., storage in the frozen state at -10°C). As a result, adult zebrafish cardiac function was successfully preserved after 5 days of partial freezing storage. In combination, the methods and techniques developed have the potential to drive and accelerate research in the fields of solid organ preservation and transplantation.
Errata / Notes
This article is corrected by ZDB-PUB-231208-2.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping