PUBLICATION
Assessment of various standard fish diets on gut microbiome of platyfish Xiphophorus maculatus
- Authors
- Soria, E., Russo, C., Carlos-Shanley, C., Drewery, M., Boswell, W., Savage, M., Sanchez, L., Chang, C., Varga, Z.M., Kent, M.L., Sharpton, T.J., Lu, Y.
- ID
- ZDB-PUB-230824-55
- Date
- 2023
- Source
- Journal of experimental zoology. Part B, Molecular and developmental evolution 342(3): 271-277 (Journal)
- Registered Authors
- Kent, Michael
- Keywords
- Xiphophorus, gut microbiome, husbandry, standardized diet
- MeSH Terms
-
- Animal Feed*
- Animals
- Cyprinodontiformes*
- Diet*/veterinary
- Gastrointestinal Microbiome*/physiology
- RNA, Ribosomal, 16S/genetics
- Zebrafish/microbiology
- PubMed
- 37614078 Full text @ J. Exp. Zool. B Mol. Dev. Evol.
Citation
Soria, E., Russo, C., Carlos-Shanley, C., Drewery, M., Boswell, W., Savage, M., Sanchez, L., Chang, C., Varga, Z.M., Kent, M.L., Sharpton, T.J., Lu, Y. (2023) Assessment of various standard fish diets on gut microbiome of platyfish Xiphophorus maculatus. Journal of experimental zoology. Part B, Molecular and developmental evolution. 342(3):271-277.
Abstract
Diet is an external factor that affects the physiological baseline of research animals. It can shape gut microbiome, which can impact the host. As a result, dietary variation can challenge experimental reproducibility and data integration across studies when not appropriately considered. To control for diet-induced variation, reference diets have been developed for common biomedical models. However, such reference diets have not yet been developed for nontraditional model organisms, such as Xiphophorus species. In this study, we compared two diets designed for zebrafish, a commercial zebrafish diet (Gemma and GEM), and a proposed zebrafish reference diet developed by the Watts laboratory at the University of Alabama at Birmingham (WAT) to the Xiphophorus Genetic Stock Center custom diet (CON) to evaluate the influence of diet on the Xiphophorus gut microbiome. Xiphophorus maculatus were fed the three diets from 2 to 6 months of age. Feces were collected and the gut microbiome was assessed using 16S rRNA sequencing every month. We observed substantial diet-driven variation in the gut microbiome. Our results indicate that diets developed specifically for zebrafish can affect the gut microbiome composition and may not be optimal for Xiphophorus.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping