PUBLICATION
Cell-type-specific responses to the microbiota across all tissues of the larval zebrafish
- Authors
- Massaquoi, M.S., Kong, G.L., Chilin-Fuentes, D., Ngo, J.S., Horve, P.F., Melancon, E., Hamilton, M.K., Eisen, J.S., Guillemin, K.
- ID
- ZDB-PUB-230215-39
- Date
- 2023
- Source
- Cell Reports 42: 112095112095 (Journal)
- Registered Authors
- Eisen, Judith S., Guillemin, Karen
- Keywords
- CP: Immunology, CP: Microbiology, development, germ-free, gnotobiology, microbiome, microbiota, single-cell atlas, transcriptomics, zebrafish
- MeSH Terms
-
- Animals
- Cell Proliferation
- Larva
- Microbiota*
- Zebrafish*
- PubMed
- 36787219 Full text @ Cell Rep.
Citation
Massaquoi, M.S., Kong, G.L., Chilin-Fuentes, D., Ngo, J.S., Horve, P.F., Melancon, E., Hamilton, M.K., Eisen, J.S., Guillemin, K. (2023) Cell-type-specific responses to the microbiota across all tissues of the larval zebrafish. Cell Reports. 42:112095112095.
Abstract
Animal development proceeds in the presence of intimate microbial associations, but the extent to which different host cells across the body respond to resident microbes remains to be fully explored. Using the vertebrate model organism, the larval zebrafish, we assessed transcriptional responses to the microbiota across the entire body at single-cell resolution. We find that cell types across the body, not limited to tissues at host-microbe interfaces, respond to the microbiota. Responses are cell-type-specific, but across many tissues the microbiota enhances cell proliferation, increases metabolism, and stimulates a diversity of cellular activities, revealing roles for the microbiota in promoting developmental plasticity. This work provides a resource for exploring transcriptional responses to the microbiota across all cell types of the vertebrate body and generating new hypotheses about the interactions between vertebrate hosts and their microbiota.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping