ZFIN ID: ZDB-PERS-011121-2
Guillemin, Karen
Email: guillemin@molbio.uoregon.edu
URL: http://morel.uoregon.edu/facres/guillemin.html
Affiliation: Guillemin Lab
Address: Institute of Molecular Biology 1229 University of Oregon Eugene, OR 97403-1229 USA
Country: United States
Phone: (541) 346-5360 (office); (541) 346-5999 (lab)
Fax: (541) 346-5891

Colonization by bacteria is an important event in normal development and is required for maturation of the gastrointestinal tract, the immune system, and for establishment of proper digestive physiology. We are interested in investigating the role of the commensal flora in zebrafish development by examining the developmental programs of animals grown axenically or with defined flora.

Bruckner, J.J., Stednitz, S.J., Grice, M.Z., Zaidan, D., Massaquoi, M.S., Larsch, J., Tallafuss, A., Guillemin, K., Washbourne, P., Eisen, J.S. (2022) The microbiota promotes social behavior by modulating microglial remodeling of forebrain neurons. PLoS Biology. 20:e3001838
Hill, J.H., Massaquoi, M.S., Sweeney, E.G., Wall, E.S., Jahl, P., Bell, R., Kallio, K., Derrick, D., Murtaugh, L.C., Parthasarathy, R., Remington, S.J., Round, J.L., Guillemin, K. (2022) BefA, a microbiota-secreted membrane disrupter, disseminates to the pancreas and increases β cell mass. Cell Metabolism. 34(11):1779-1791.e9
Sundarraman, D., Smith, T.J., Kast, J.V.Z., Guillemin, K., Parthasarathy, R. (2022) Disaggregation as an interaction mechanism among intestinal bacteria. Biophysical journal. 121(18):3458-3473
Jawahar, J., McCumber, A.W., Lickwar, C.R., Amoroso, C.R., de la Torre Canny, S.G., Wong, S., Morash, M., Thierer, J.H., Farber, S.A., Bohannan, B.J.M., Guillemin, K., Rawls, J.F. (2022) Starvation causes changes in the intestinal transcriptome and microbiome that are reversed upon refeeding. BMC Genomics. 23:225
Hamilton, M.K., Wall, E.S., Robinson, C.D., Guillemin, K., Eisen, J.S. (2022) Enteric nervous system modulation of luminal pH modifies the microbial environment to promote intestinal health. PLoS pathogens. 18:e1009989
Robinson, C.D., Sweeney, E.G., Ngo, J., Ma, E., Perkins, A., Smith, T.J., Fernandez, N.L., Waters, C.M., Remington, S.J., Bohannan, B.J.M., Guillemin, K. (2021) Host-emitted amino acid cues regulate bacterial chemokinesis to enhance colonization. Cell Host & Microbe. 29(8):1221-1234.e8
Hinman, M.N., Richardson, J.I., Sockol, R.A., Aronson, E.D., Stednitz, S.J., Murray, K.N., Berglund, J.A., Guillemin, K. (2021) Zebrafish mbnl mutants model physical and molecular phenotypes of myotonic dystrophy. Disease models & mechanisms. 14(6):
Kent, M.L., Wall, E.S., Sichel, S., Watral, V., Stagaman, K., Sharpton, T.J., Guillemin, K. (2021) Pseudocapillaria tomentosa, Mycoplasma spp., and Intestinal Lesions in Experimentally Infected Zebrafish Danio rerio. Zebrafish. 18(3):207-220
Postlethwait, J.H., Massaquoi, M.S., Farnsworth, D.R., Yan, Y.L., Guillemin, K., Miller, A.C. (2021) The SARS-CoV-2 receptor and other key components of the Renin-Angiotensin-Aldosterone System related to COVID-19 are expressed in enterocytes in larval zebrafish. Biology Open. 10(3):
Loes, A.N., Hinman, M.N., Farnsworth, D.R., Miller, A.C., Guillemin, K., Harms, M.J. (2021) Identification and Characterization of Zebrafish Tlr4 Coreceptor Md-2. Journal of immunology (Baltimore, Md. : 1950). 206(5):1046-1057
Solis, C.J., Hamilton, M.K., Caruffo, M., Garcia-Lopez, J.P., Navarrete, P., Guillemin, K., Feijoo, C.G. (2020) Intestinal Inflammation Induced by Soybean Meal Ingestion Increases Intestinal Permeability and Neutrophil Turnover Independently of Microbiota in Zebrafish. Frontiers in immunology. 11:1330
Stagaman, K., Sharpton, T.J., Guillemin, K. (2020) Zebrafish microbiome studies make waves. Lab animal. 49(7):201-207
Wiles, T.J., Schlomann, B.H., Wall, E.S., Betancourt, R., Parthasarathy, R., Guillemin, K. (2020) Swimming motility of a gut bacterial symbiont promotes resistance to intestinal expulsion and enhances inflammation. PLoS Biology. 18:e3000661
Schlomann, B.H., Wiles, T.J., Wall, E.S., Guillemin, K., Parthasarathy, R. (2019) Sublethal antibiotics collapse gut bacterial populations by enhancing aggregation and expulsion. Proceedings of the National Academy of Sciences of the United States of America. 116(43):21392-21400
Chia, J.S.M., Wall, E.S., Wee, C.L., Rowland, T.A.J., Cheng, R.K., Cheow, K., Guillemin, K., Jesuthasan, S. (2019) Bacteria evoke alarm behaviour in zebrafish. Nature communications. 10:3831
Wiles, T.J., Guillemin, K. (2019) The Other Side of the Coin: What Beneficial Microbes Can Teach us about Pathogenic Potential. Journal of molecular biology. 431(16):2946-2956
Robinson, C.D., Klein, H.S., Murphy, K.D., Parthasarathy, R., Guillemin, K., Bohannan, B.J.M. (2018) Experimental bacterial adaptation to the zebrafish gut reveals a primary role for immigration. PLoS Biology. 16:e2006893
Rolig, A.S., Sweeney, E.G., Kaye, L.E., DeSantis, M.D., Perkins, A., Banse, A.V., Hamilton, M.K., Guillemin, K. (2018) A bacterial immunomodulatory protein with lipocalin-like domains facilitates host-bacteria mutualism in larval zebrafish. eLIFE. 7:
Schlomann, B.H., Wiles, T.J., Wall, E.S., Guillemin, K., Parthasarathy, R. (2018) Bacterial Cohesion Predicts Spatial Distribution in the Larval Zebrafish Intestine. Biophysical journal. 115(11):2271-2277
Wiles, T.J., Wall, E.S., Schlomann, B.H., Hay, E.A., Parthasarathy, R., Guillemin, K. (2018) Modernized Tools for Streamlined Genetic Manipulation and Comparative Study of Wild and Diverse Proteobacterial Lineages. mBio. 9(5)
Troll, J.V., Hamilton, M.K., Abel, M.L., Ganz, J., Bates, J.M., Stephens, W.Z., Melancon, E., van der Vaart, M., Meijer, A.H., Distel, M., Eisen, J.S., Guillemin, K. (2018) Microbiota promote secretory cell determination in the intestinal epithelium by modulating host Notch signaling. Development (Cambridge, England). 145(4)
Burns, A.R., Miller, E., Agarwal, M., Rolig, A.S., Milligan-Myhre, K., Seredick, S., Guillemin, K., Bohannan, B.J.M. (2017) Interhost dispersal alters microbiome assembly and can overwhelm host innate immunity in an experimental zebrafish model. Proceedings of the National Academy of Sciences of the United States of America. 114(42):11181-11186
Burns, A.R., Watral, V., Sichel, S., Spagnoli, S., Banse, A.V., Mittge, E., Sharpton, T.J., Guillemin, K., Kent, M.L. (2017) Transmission of a common intestinal neoplasm in zebrafish by cohabitation. Journal of fish diseases. 41(4):569-579
Stagaman, K., Burns, A.R., Guillemin, K., Bohannan, B.J. (2017) The role of adaptive immunity as an ecological filter on the gut microbiota in zebrafish. The ISME Journal. 11:1630-1639
Burns, A.R., Guillemin, K. (2017) The scales of the zebrafish: host-microbiota interactions from proteins to populations. Current opinion in microbiology. 38:137-141
Rolig, A.S., Mittge, E.K., Ganz, J., Troll, J.V., Melancon, E., Wiles, T.J., Alligood, K., Stephens, W.Z., Eisen, J.S., Guillemin, K. (2017) The enteric nervous system promotes intestinal health by constraining microbiota composition. PLoS Biology. 15:e2000689
Melancon, E., Gomez De La Torre Canny, S., Sichel, S., Kelly, M., Wiles, T.J., Rawls, J.F., Eisen, J.S., Guillemin, K. (2017) Best practices for germ-free derivation and gnotobiotic zebrafish husbandry. Methods in cell biology. 138:61-100
Hill, J.H., Franzosa, E.A., Huttenhower, C., Guillemin, K. (2016) A conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development. eLIFE. 5
Wiles, T.J., Jemielita, M., Baker, R.P., Schlomann, B.H., Logan, S.L., Ganz, J., Melancon, E., Eisen, J.S., Guillemin, K., Parthasarathy, R. (2016) Host Gut Motility Promotes Competitive Exclusion within a Model Intestinal Microbiota. PLoS Biology. 14:e1002517
Burns, A.R., Zac Stephens, W., Stagaman, K., Wong, S., Rawls, J.F., Guillemin, K., Bohannan, B.J. (2016) Contribution of neutral processes to the assembly of gut microbial communities in the zebrafish over host development. The ISME Journal. 10(3):655-64
Zac Stephens, W., Burns, A.R., Stagaman, K., Wong, S., Rawls, J.F., Guillemin, K., Bohannan, B.J. (2016) The composition of the zebrafish intestinal microbial community varies across development. The ISME Journal. 10(3):644-54
Rolig, A.S., Parthasarathy, R., Burns, A.R., Bohannan, B.J., Guillemin, K. (2015) Individual Members of the Microbiota Disproportionately Modulate Host Innate Immune Responses. Cell Host & Microbe. 18:613-20
Stephens, W.Z., Wiles, T.J., Martinez, E.S., Jemielita, M., Burns, A.R., Parthasarathy, R., Bohannan, B.J., Guillemin, K. (2015) Identification of Population Bottlenecks and Colonization Factors during Assembly of Bacterial Communities within the Zebrafish Intestine. mBio. 6(6):e01163-15
Wong, S., Stephens, W.Z., Burns, A.R., Stagaman, K., David, L.A., Bohannan, B.J., Guillemin, K., Rawls, J.F. (2015) Ontogenetic Differences in Dietary Fat Influence Microbiota Assembly in the Zebrafish Gut. mBio. 6(5):e00687-15
Yang, Y., Millán, J.L., Mecsas, J., Guillemin, K. (2015) Intestinal alkaline phosphatase deficiency leads to lipopolysaccharide desensitization and faster weight gain. Infection and Immunity. 83(1):247-58
Jemielita, M., Taormina, M.J., Burns, A.R., Hampton, J.S., Rolig, A.S., Guillemin, K., Parthasarathy, R. (2014) Spatial and Temporal Features of the Growth of a Bacterial Species Colonizing the Zebrafish Gut. mBio. 5(6):1698-710
Paquette, C.E., Kent, M.L., Buchner, C., Tanguay, R.L., Guillemin, K., Mason, T.J., and Peterson, T.S. (2013) A Retrospective Study of the Prevalence and Classification of Intestinal Neoplasia in Zebrafish (Danio rerio). Zebrafish. 10(2):228-36
Neal, J.T., Peterson, T.S., Kent, M.L., and Guillemin, K. (2013) H. pylori Virulence Factor CagA Increases Intestinal Cell Proliferation by Wnt Pathway Activation in a Transgenic Zebrafish Model. Disease models & mechanisms. 6(3):802-10
Yang, Y., Wandler, A.M., Postlethwait, J.H., and Guillemin, K. (2012) Dynamic Evolution of the LPS-Detoxifying Enzyme Intestinal Alkaline Phosphatase in Zebrafish and Other Vertebrates. Frontiers in Molecular Innate Immunity. 3:314
Taormina, M.J., Jemielita, M., Stephens, W.Z., Burns, A.R., Troll, J.V., Parthasarathy, R., and Guillemin, K. (2012) Investigating bacterial-animal symbioses with light sheet microscopy. The Biological bulletin. 223(1):7-20
Roeselers, G., Mittge, E.K., Stephens, W.Z., Parichy, D.M., Cavanaugh, C.M., Guillemin, K., and Rawls, J.F. (2011) Evidence for a core gut microbiota in the zebrafish. The ISME Journal. 5(10):1595-608
Cheesman, S.E., Neal, J.T., Mittge, E., Seredick, B.M., and Guillemin, K. (2011) Epithelial cell proliferation in the developing zebrafish intestine is regulated by the Wnt pathway and microbial signaling via Myd88. Proceedings of the National Academy of Sciences of the United States of America. 108(Suppl 1):4570-4577
Milligan-Myhre, K., Charette, J.R., Phennicie, R.T., Stephens, W.Z., Rawls, J.F., Guillemin, K., and Kim, C.H. (2011) Study of host-microbe interactions in zebrafish. Methods in cell biology. 105:87-116
Bates, J.M., Akerlund, J., Mittge, E., and Guillemin, K. (2007) Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota. Cell Host & Microbe. 2(6):371-382
Baden, K.N., Murray, J., Capaldi, R.A., and Guillemin, K. (2007) Early developmental pathology due to cytochrome c oxidase deficiency is revealed by a new zebrafish model. The Journal of biological chemistry. 282(48):34839-34849
Cheesman, S.E., and Guillemin, K. (2007) We know you are in there: Conversing with the indigenous gut microbiota. Research in Microbiology. 158(1):2-9
Bates, J.M., Mittge, E., Kuhlman, J., Baden, K.N., Cheesman, S.E., and Guillemin, K. (2006) Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation. Developmental Biology. 297(2):374-386

Guillemin, K., Williams, T., and Krasnow, M.A. (2001) A Nuclear Lamin is Required for Cytoplasmic Organization and Egg Polarity in Drosophila. Nature Cell Biology, Nature Cell Biology 9:848-51.

Guillemin, K., Salama, N., McDaniel, T. K., Sherlock, G, Tompkins, L and Falkow, S. (2000) A Whole Genome Microarray Reveals Genetic Diversity Among Helicobacter Pylori Strains. PNAS 97: 14668-14673.

Guillemin, K. and Krasnow, M. A. (1997) The Hypoxic Response: Huffing and HIFing. Cell 89: 9-12.

Guillemin, K., Groppe, J., Dücker, K., Treisman, R., Hafen, E., Affolter, M. and Krasnow, M. A. (1996) The pruned gene encodes the Drosophila serum response factor and regulates cytoplasmic outgrowth during terminal branching of the tracheal system. Development 122(5): 1353-1362.