PUBLICATION
Cilia regulate meiotic recombination in zebrafish
- Authors
- Xie, H., Wang, X., Jin, M., Li, L., Zhu, J., Kang, Y., Chen, Z., Sun, Y., Zha, C.
- ID
- ZDB-PUB-220819-13
- Date
- 2022
- Source
- Journal of molecular cell biology 14(7): (Journal)
- Registered Authors
- Sun, Yonghua, Zhao, Chengtian
- Keywords
- kif3a, cilia, homologous recombination, meiosis, zebrafish
- MeSH Terms
-
- Animals
- Chromosome Pairing
- Cilia*
- DNA Repair
- Male
- Meiosis
- Zebrafish*
- PubMed
- 35981808 Full text @ J. Mol. Cell Biol.
Citation
Xie, H., Wang, X., Jin, M., Li, L., Zhu, J., Kang, Y., Chen, Z., Sun, Y., Zha, C. (2022) Cilia regulate meiotic recombination in zebrafish. Journal of molecular cell biology. 14(7).
Abstract
Meiosis is essential for evolution and genetic diversity in almost all sexual eukaryotic organisms. The mechanisms of meiotic recombination, such as synapsis, have been extensively investigated. However, it is still unclear whether signals from the cytoplasm or even from outside of the cell can regulate the meiosis process. Cilia are microtubule-based structures that protrude from cell surface and function as signaling hubs to sense extracellular signals. Here, we reported an unexpected and critical role of cilia during meiotic recombination. During gametogenesis of zebrafish, cilia were specifically present in the prophase stages of both primary spermatocytes and primary oocytes. By developing a germ cell-specific CRISPR/Cas9 system, we demonstrated that germ cell-specific depletion of ciliary genes resulted in compromised double-strand break repair, reduced crossover formation, and increased germ cell apoptosis. Our study reveals a previously undiscovered role for cilia during meiosis and suggests that extracellular signals may regulate meiotic recombination via this particular organelle.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping