PUBLICATION
Power-effective scanning with AODs for 3D optogenic applications
- Authors
- Ricci, P., Marchetti, M., Sorelli, M., Turrini, L., Resta, F., Gavryusev, V., de Vito, G., Sancataldo, G., Vanzi, F., Silvestri, L., Pavone, F.S.
- ID
- ZDB-PUB-220111-5
- Date
- 2022
- Source
- Journal of biophotonics 15(4): e202100256 (Journal)
- Registered Authors
- Turrini, Lapo, Vanzi, Francesco
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain/diagnostic imaging
- Neurons*
- Optogenetics
- Photic Stimulation/methods
- Zebrafish*
- PubMed
- 35000289 Full text @ J. Biophotonics
Citation
Ricci, P., Marchetti, M., Sorelli, M., Turrini, L., Resta, F., Gavryusev, V., de Vito, G., Sancataldo, G., Vanzi, F., Silvestri, L., Pavone, F.S. (2022) Power-effective scanning with AODs for 3D optogenic applications. Journal of biophotonics. 15(4):e202100256.
Abstract
Two-photon (2P) excitation is a cornerstone approach widely employed in neuroscience microscopy for deep optical access and sub-micrometric resolution light-targeting into the brain. However, besides structural and functional imaging, 2P optogenetic stimulations are less routinary, especially in 3D. This is because of the adopted scanning systems, often feebly effective, slow and mechanically constricted. Faster illumination can be achieved through acousto-optic deflectors (AODs) although their applicability to large volumes excitation has been limited by large efficiency drop along the optical axis. Here, we present a new AOD-based scheme for 2P 3D-scanning that improves the power delivery between different illumination planes. We applied this approach to photostimulate an optogenetic actuator in zebrafish larvae, demonstrating the method efficiency observing increased activity responses and uniform activation probabilities from neuronal clusters addressed in the volume. This novel driving scheme can open to new AOD applications in neuroscience, allowing more effective 3D-interrogation in large neuronal networks. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping