PUBLICATION
Label-free quantitative measurement of cardiovascular dynamics in a zebrafish embryo using frequency-comb-referenced-quantitative phase imaging
- Authors
- Boonruangkan, J., Farrokhi, H., Rohith, T.M., Kwok, S., Carney, T.J., Su, P.C., Kim, Y.J.
- ID
- ZDB-PUB-211116-22
- Date
- 2021
- Source
- Journal of Biomedical Optics 26(11): (Journal)
- Registered Authors
- Carney, Tom, Kwok, Samuel
- Keywords
- cardiovascular dynamics, frequency comb, high-speed phase measurement, quantitative phase imaging, zebrafish
- MeSH Terms
-
- Animals
- Diagnostic Imaging
- Embryo, Mammalian
- Embryo, Nonmammalian
- Heart Rate
- Hemodynamics*
- Zebrafish*
- PubMed
- 34773396 Full text @ J. Biomed. Opt.
Citation
Boonruangkan, J., Farrokhi, H., Rohith, T.M., Kwok, S., Carney, T.J., Su, P.C., Kim, Y.J. (2021) Label-free quantitative measurement of cardiovascular dynamics in a zebrafish embryo using frequency-comb-referenced-quantitative phase imaging. Journal of Biomedical Optics. 26(11):.
Abstract
Significance Real-time monitoring of the heart rate and blood flow is crucial for studying cardiovascular dysfunction, which leads to cardiovascular diseases.
Aim This study aims at in-depth understanding of high-speed cardiovascular dynamics in a zebrafish embryo model for various biomedical applications via frequency-comb-referenced quantitative phase imaging (FCR-QPI).
Approach Quantitative phase imaging (QPI) has emerged as a powerful technique in the field of biomedicine but has not been actively applied to the monitoring of circulatory/cardiovascular parameters, due to dynamic speckles and low frame rates. We demonstrate FCR-QPI to measure heart rate and blood flow in a zebrafish embryo. FCR-QPI utilizes a high-speed photodetector instead of a conventional camera, so it enables real-time monitoring of individual red blood cell (RBC) flow.
Results The average velocity of zebrafish's RBCs was measured from 192.5 to 608.8 μm / s at 24 to 28 hour-post-fertilization (hpf). In addition, the number of RBCs in a pulsatile blood flow was revealed to 16 cells/pulse at 48 hpf. The heart rates corresponded to 94 and 142 beats-per-minute at 24 and 48 hpf.
Conclusions This approach will newly enable in-depth understanding of the cardiovascular dynamics in the zebrafish model and possible usage for drug discovery applications in biomedicine.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping