PUBLICATION

Global Transcriptomic Analysis of Zebrafish Glucagon Receptor Mutant Reveals Its Regulated Metabolic Network

Authors
Kang, Q., Hu, M., Jia, J., Bai, X., Liu, C., Wu, Z., Chen, W., Li, M.
ID
ZDB-PUB-200128-1
Date
2020
Source
International Journal of Molecular Sciences   21(3): (Journal)
Registered Authors
Chen, Wenbiao, Li, Mingyu
Keywords
RNA sequencing, diabetes, glucagon receptor, metabolic network, zebrafish
MeSH Terms
  • Animals
  • Gene Expression Profiling/methods
  • Glucose/genetics
  • Glucose/metabolism
  • Liver/metabolism
  • Liver/physiology
  • Metabolic Networks and Pathways/genetics*
  • Receptors, Glucagon/genetics*
  • Receptors, Glucagon/metabolism
  • Signal Transduction/genetics
  • Transcriptome/genetics*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed
31979106 Full text @ Int. J. Mol. Sci.
Abstract
The glucagon receptor (GCGR) is a G-protein-coupled receptor (GPCR) that mediates the activity of glucagon. Disruption of GCGR results in many metabolic alterations, including increased glucose tolerance, decreased adiposity, hypoglycemia, and pancreatic α-cell hyperplasia. To better understand the global transcriptomic changes resulting from GCGR deficiency, we performed whole-organism RNA sequencing analysis in wild type and gcgr-deficient zebrafish. We found that the expression of 1645 genes changes more than two-fold among mutants. Most of these genes are related to metabolism of carbohydrates, lipids, and amino acids. Genes related to fatty acid β-oxidation, amino acid catabolism, and ureagenesis are often downregulated. Among gcrgr-deficient zebrafish, we experimentally confirmed increases in lipid accumulation in the liver and whole-body glucose uptake, as well as a modest decrease in total amino acid content. These results provide new information about the global metabolic network that GCGR signaling regulates in addition to a better understanding of the receptor's physiological functions.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes