PUBLICATION
Proteomic Analysis of Zebrafish (Danio rerio) After Chemical Exposure
- Authors
- Lee, Y.M., Li, C., Lam, S.H., Gong, Z., Lin, Q.
- ID
- ZDB-PUB-180614-13
- Date
- 2018
- Source
- Methods in molecular biology (Clifton, N.J.) 1797: 443-459 (Chapter)
- Registered Authors
- Gong, Zhiyuan, Lam, Siew Hong
- Keywords
- 2D-LC-MS/MS, Proteomics, Quantitative proteomics, Shotgun, TCDD, Teratogen, Zebrafish, iTRAQ
- MeSH Terms
-
- Animals
- Chromatography, Liquid
- Embryo, Nonmammalian
- Environmental Exposure
- Mass Spectrometry
- Polychlorinated Dibenzodioxins/pharmacology
- Proteome/drug effects*
- Proteomics*/methods
- Teratogens/pharmacology*
- Zebrafish/metabolism*
- Zebrafish Proteins/metabolism
- PubMed
- 29896708 Full text @ Meth. Mol. Biol.
Citation
Lee, Y.M., Li, C., Lam, S.H., Gong, Z., Lin, Q. (2018) Proteomic Analysis of Zebrafish (Danio rerio) After Chemical Exposure. Methods in molecular biology (Clifton, N.J.). 1797:443-459.
Abstract
Traditional toxicological screens based on the zebrafish model use observable phenotypic endpoints during their development to determine the toxicity of teratogens. Yet toxicity does not always translate to obvious phenotypic changes and the criteria used to score the toxicity of a teratogen are frequently subjected to human perception. The advancement in omics-based technologies has allowed us to quantitatively and objectively determine the toxicity of a teratogen based on biomolecular changes. The field of proteomics has been gaining popularity as a valuable tool in toxicology. Hence, in this chapter, we described a protocol for both label-free and label-based proteomic methods to analyse proteomic changes in both embryos and adult livers of zebrafish exposed to the teratogen TCDD (tetrachlorodibenzo-p-dioxin) as an example.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping