Walters, J.W., Anderson, J.L., Bittman, R., Pack, M., and Farber, S.A. (2012) RETRACTED: Visualization of lipid metabolism in the zebrafish intestine reveals a relationship between NPC1L1-mediated cholesterol uptake and dietary fatty acid. Chemistry & Biology. 19:913-925.
In our original Resource, we reported a regulatory link between NPC1L1-mediated cholesterol uptake and dietary fatty acid in zebrafish larvae. In the absence of dietary long-chain fatty acids (LCFA), larval enterocytes failed to internalize luminal BODIPY-cholesterol. We attributed this LCFA dependence to the cholesterol transport protein NPC1L1, as dietary LCFAs were shown to induce the translocation of transgenic human NPC1L1 from a perinuclear compartment to the intestinal brush border (supported by the data shown in Figure 5 of the Resource). When overexpressed, human NPC1L1 localized to the brush border, where it was able to mediate the internalization of BODIPY-cholesterol even in the absence of dietary LCFAs (Figure 5). We also reported that NPC1L1 directly mediates cholesterol uptake by larval enterocytes (shown in Figure 6 of the Resource). However, we recently discovered that the larval expression of the human NPC1L1 transgenic construct hsp70:HsNPC1L1-mCherry we reported was in fact that of hsp70:mCherryCAAX, which encodes an mCherry fluorophore modified with a prenylation motif. When employing a recloned hsp70:HsNPC1L1-mCherry vector, our experiments failed to replicate the LCFA-induced translocation of NPC1L1 to the intestinal brush border, indicating that the findings reported in Figures 5 and 6 are no longer valid.
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