ZFIN Person: Pack, Michael

ZFIN ID: ZDB-PERS-960805-421

Pack, Michael

Email:	mpack@mail.med.upenn.edu
URL:
Affiliation:	Pack Lab
Address:	Department of Medicine University of Pennsylvania School of Medicine 1230 Biomedical Research Building II/III 421 Curie Boulevard Philadelphia, PA 19104 USA
Country:	United States
Phone:	(215) 573-4145
Fax:	(215) 573-7149
ORCID ID:

BIOGRAPHY AND RESEARCH INTERESTS

Digestive organ development (intestine, pancreas and liver).

PUBLICATIONS

Liu, X., Jones, W.D., Quesnel-Vallières, M., Devadiga, S.A., Lorent, K., Valvezan, A.J., Myers, R.L., Li, N., Lengner, C.J., Barash, Y., Pack, M., Klein, P.S. (2023) The Tumor Suppressor Adenomatous Polyposis Coli (apc) Is Required for Neural Crest-Dependent Craniofacial Development in Zebrafish. Journal of developmental biology. 11(3):

Bedell, V.M., Meng, Q.C., Pack, M.A., Eckenhoff, R.G. (2020) A vertebrate model to reveal neural substrates underlying the transitions between conscious and unconscious states. Scientific Reports. 10:15789

Zhao, X., Lorent, K., Escobar-Zarate, D., Rajagopalan, R., Loomes, K.M., Gillespie, K., Mesaros, C., Estrada, M.A., Blair, I., Winkler, J.D., Spinner, N.B., Devoto, M., Pack, M. (2020) Impaired redox and protein homeostasis as risk factors and therapeutic targets in toxin-induced biliary atresia. Gastroenterology. 159(3):1068-1084.e2

Ye, S., Liu, Y., Fuller, A.M., Katti, R., Ciotti, G.E., Chor, S., Alam, M.Z., Devalaraja, S., Lorent, K., Weber, K., Haldar, M., Pack, M.A., Eisinger-Mathason, T.S.K. (2020) TGFβ and Hippo pathways cooperate to enhance sarcomagenesis and metastasis through the hyaluronan mediated motility receptor (HMMR). Molecular cancer research : MCR. 18(4):560-573

McKinstry-Wu, A.R., Wasilczuk, A.Z., Harrison, B.A., Bedell, V.M., Sridharan, M.J., Breig, J.J., Pack, M., Kelz, M.B., Proekt, A. (2019) Analysis of stochastic fluctuations in responsiveness is a critical step toward personalized anesthesia. eLIFE. 8:

Ko, Y.A., Yi, H., Qiu, C., Huang, S., Park, J., Ledo, N., Köttgen, A., Li, H., Rader, D.J., Pack, M.A., Brown, C.D., Susztak, K. (2017) Genetic-Variation-Driven Gene-Expression Changes Highlight Genes with Important Functions for Kidney Disease. American journal of human genetics. 100:940-953

Zhao, X., Pack, M. (2017) Modeling intestinal disorders using zebrafish. Methods in cell biology. 138:241-270

Zhao, X., Lorent, K., Wilkins, B., Marchione, D.M., Gillespie, K., Waisbourd-Zinman, O., So, J., Koo, K.A., Shin, D., Porter, J.R., Wells, R.G., Blair, I., Pack, M. (2016) Glutathione antioxidant pathway activity and reserve determine toxicity and specificity of the biliary toxin biliatresone in zebrafish. Hepatology (Baltimore, Md.). 64(3):894-907

Abrams, J., Einhorn, Z., Seiler, C., Zong, A.B., Sweeney, H.L., Pack, M. (2016) Graded effects of unregulated smooth muscle myosin on intestinal architecture, intestinal motility, and vascular function in zebrafish. Disease models & mechanisms. 9(5):529-40

Koo, K.A., Lorent, K., Gong, W., Windsor, P., Whittaker, S.J., Pack, M., Wells, R., Porter, J.R. (2015) Biliatresone, A Reactive Natural Toxin from Dysphania glomulifera and D. littoralis: Discovery of the Toxic Moiety 1,2-Diaryl-2-Propenone. Chemical Research in Toxicology. 28(8):1519-21

Pack, M. (2015) IBD. Fishing for missing heritability in IBD. Nature reviews. Gastroenterology & hepatology. 12:318-20

Lorent, K., Gong, W., Koo, K.A., Waisbourd-Zinman, O., Karjoo, S., Zhao, X., Sealy, I., Kettleborough, R.N., Stemple, D.L., Windsor, P.A., Whittaker, S.J., Porter, J.R., Wells, R.G., Pack, M. (2015) Identification of a plant isoflavonoid that causes biliary atresia. Science Translational Medicine. 7:286ra67

Wilkins, B.J., Gong, W., and Pack, M. (2014) A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver. Gene expression patterns : GEP. 14(2):62-68

Valvezan, A.J., Huang, J., Lengner, C.J., Pack, M., and Klein, P.S. (2014) Oncogenic mutations in Adenomatous Polyposis Coli (Apc) activate mechanistic Target of Rapamycin Complex 1 (mTORC1). Disease models & mechanisms. 7(1):63-71

Wilkins, B.J., Lorent, K., Matthews, R.P., and Pack, M. (2013) p53-Mediated Biliary Defects Caused by Knockdown of cirh1a, the Zebrafish Homolog of the Gene Responsible for North American Indian Childhood Cirrhosis. PLoS One. 8(10):e77670

Rawnsley, D.R., Xiao, J., Lee, J.S., Liu, X., Mericko-Ishizuka, P., Kumar, V., He, J., Basu, A., Lu, M., Lynn, F.C., Pack, M., Gasa, R., Kahn, M.L. (2013) The transcription factor Atonal homolog 8 regulates Gata4 and Friend of Gata-2 during vertebrate development. The Journal of biological chemistry. 288:24429-40

Wilkins, B.J., and Pack, M. (2013) Zebrafish models of human liver development and disease. Comprehensive Physiology. 3(3):1213-1230

Walters, J.W., Anderson, J.L., Bittman, R., Pack, M., and Farber, S.A. (2012) RETRACTION: Visualization of lipid metabolism in the zebrafish intestine reveals a relationship between NPC1L1-mediated cholesterol uptake and dietary fatty acid. Chemistry & Biology. 19:913-925

Abrams, J., Davuluri, G., Seiler, C., and Pack, M. (2012) Smooth muscle caldesmon modulates peristalsis in the wild type and non-innervated zebrafish intestine. Neurogastroenterology and motility. 24(3):288-299

Seiler, C., Davuluri, G., Abrams, J., Byfield, F.J., Janmey, P.A., and Pack, M. (2012) Smooth muscle tension induces invasive remodeling of the zebrafish intestine. PLoS Biology. 10(9):e1001386

Matthews, R.P., Eauclaire, S.F., Mugnier, M., Lorent, K., Cui, S., Ross, M.M., Zhang, Z., Russo, P., and Pack, M. (2011) DNA hypomethylation causes bile duct defects in zebrafish and is a distinguishing feature of infantile biliary atresia. Hepatology (Baltimore, Md.). 53(3):905-914

Seiler, C., and Pack, M. (2011) Transgenic labeling of the zebrafish pronephric duct and tubules using a promoter from the enpep gene. Gene expression patterns : GEP. 11(1-2):118-121

Seiler, C., Abrams, J., and Pack, M. (2010) Characterization of zebrafish intestinal smooth muscle development using a novel sm22{alpha}-b promoter. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(11):2806-2812

Davuluri, G., Seiler, C., Abrams, J., Soriano, A.J., and Pack, M. (2010) Differential effects of thin and thick filament disruption on zebrafish smooth muscle regulatory proteins. Neurogastroenterology and motility. 22(10):1100-e285

Clifton, J.D., Lucumi, E., Myers, M.C., Napper, A., Hama, K., Farber, S.A., Smith, A.B. 3rd, Huryn, D.M., Diamond, S.L., and Pack, M. (2010) Identification of novel inhibitors of dietary lipid absorption using zebrafish. PLoS One. 5(8):e12386

Zheng, X., Xu, C., Di Lorenzo, A., Kleaveland, B., Zou, Z., Seiler, C., Chen, M., Cheng, L., Xiao, J., He, J., Pack, M.A., Sessa, W.C., and Kahn, M.L. (2010) CCM3 signaling through sterile 20-like kinases plays an essential role during zebrafish cardiovascular development and cerebral cavernous malformations. J. Clin. Invest.. 120(8):2795-2804

Lorent, K., Moore, J.C., Siekmann, A.F., Lawson, N., and Pack, M. (2010) Reiterative use of the notch signal during zebrafish intrahepatic biliary development. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(3):855-864

Matthews, R.P., Lorent, K., Mañoral-Mobias, R., Huang, Y., Gong, W., Murray, I.V., Blair, I.A., and Pack, M. (2009) TNF{alpha}-dependent hepatic steatosis and liver degeneration caused by mutation of zebrafish s-adenosylhomocysteine hydrolase. Development (Cambridge, England). 136(5):865-875

Davuluri, G., Gong, W., Yusuff, S., Lorent, K., Muthumani, M., Dolan, A.C., and Pack, M. (2008) Mutation of the zebrafish nucleoporin elys sensitizes tissue progenitors to replication stress. PLoS Genetics. 4(10):e1000240

Graziotto, J.J., Inglehearn, C.F., Pack, M.A., and Pierce, E.A. (2008) Decreased Levels of the RNA Splicing Factor Prpf3 in Mice and Zebrafish Do Not Cause Photoreceptor Degeneration. Investigative ophthalmology & visual science. 49(9):3830-3838

Lucitt, M.B., Price, T.S., Pizarro, A., Wu, W., Yocum, A.K., Seiler, C., Pack, M.A., Blair, I.A., Fitzgerald, G.A., and Grosser, T. (2008) Analysis of the zebrafish proteome during embryonic development. Molecular & cellular proteomics : MCP. 7(5):981-994

Matthews, R.P., Lorent, K., and Pack, M. (2008) Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(1):124-131

Chen, X., Moorman, S.J., Wallace, K.N., Pack, M., and Haendel, M.A. (2007) ZFIN Anatomy Working Group: Digestive System. Manually curated data.

Ho, S.Y., Lorent, K., Pack, M., and Farber, S.A. (2006) Zebrafish fat-free is required for intestinal lipid absorption and Golgi apparatus structure. Cell Metabolism. 3(4):289-300

Matthews, R.P., Plumb-Rudewiez, N., Lorent, K., Gissen, P., Johnson, C.A., Lemaigre, F., and Pack, M. (2005) Zebrafish vps33b, an ortholog of the gene responsible for human arthrogryposis-renal dysfunction-cholestasis syndrome, regulates biliary development downstream of the onecut transcription factor hnf6. Development (Cambridge, England). 132(23):5295-5306

Yee, N.S., Lorent, K., and Pack, M. (2005) Exocrine pancreas development in zebrafish. Developmental Biology. 284(1):84-101

Wallace, K.N., Dolan, A.C., Seiler, C., Smith, E.M., Yusuff, S., Chaille-Arnold, L., Judson, B., Sierk, R., Yengo, C., Sweeney, H.L., and Pack, M. (2005) Mutation of smooth muscle Myosin causes epithelial invasion and cystic expansion of the zebrafish intestine. Developmental Cell. 8(5):717-726

Wallace, K.N., Akhter, S., Smith, E.M., Lorent, K., and Pack, M. (2005) Intestinal growth and differentiation in zebrafish. Mechanisms of Development. 122(2):157-73

Pini, B., Grosser, T., Lawson, J.A., Price, T.S., Pack, M.A., and Fitzgerald, G.A. (2005) Prostaglandin E Synthases in Zebrafish. Arterioscler. Thromb. Vasc. Biol.. 25(2):315-320

Yee, N.S., and Pack, M. (2005) Zebrafish as a model for pancreatic cancer research. Methods in molecular medicine. 103:273-298

Lorent, K., Yeo, S.Y., Oda, T., Chandrasekharappa, S., Chitnis, A., Matthews, R.P., and Pack, M. (2004) Inhibition of Jagged-mediated Notch signaling disrupts zebrafish biliary development and generates multi-organ defects compatible with an Alagille syndrome phenocopy. Development (Cambridge, England). 131(22):5753-5766

Matthews, R.P., Lorent, K., Russo, P., and Pack, M. (2004) The zebrafish onecut gene hnf-6 functions in an evolutionarily conserved genetic pathway that regulates vertebrate biliary development. Developmental Biology. 274(2):245-259

Esni, F., Ghosh, B., Biankin, A.V., Lin, J.W., Albert, M.A., Yu, X., MacDonald, R.J., Civin, C.I., Real, F.X., Pack, M.A., Ball, D.W., and Leach, S.D. (2004) Notch inhibits Ptf1 function and acinar cell differentiation in developing mouse and zebrafish pancreas. Development (Cambridge, England). 131(17):4213-4224

Lin, J.W., Biankin, A.V., Horb, M.E., Ghosh, B., Prasad, N.B., Yee, N.S., Pack, M.A., and Leach, S.D. (2004) Differential requirement for ptf1a in endocrine and exocrine lineages of developing zebrafish pancreas. Developmental Biology. 270(2):474-486

Wallace, K.N. and Pack, M. (2003) Unique and conserved aspects of gut development in zebrafish. Developmental Biology. 255(1):12-29

Ho, S.Y., Pack, M., and Farber, S.A. (2003) Analysis of small molecule metabolism in zebrafish. Methods in enzymology. 364:408-426

Grosser, T., Yusuff, S., Cheskis, E., Pack, M.A., and FitzGerald, G.A. (2002) Developmental expression of functional cyclooxygenases in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 99(12):8418-8423

Wallace, K.N., Yusuff, S., Sonntag, J.M., Chin, A.J., and Pack, M. (2001) Zebrafish hhex regulates liver development and digestive organ chirality. Genesis (New York, N.Y. : 2000). 30(3):141-3

Yee, N.S., Yusuff, S., and Pack, M. (2001) Zebrafish pdx1 morphant displays defects in pancreas development and digestive organ chirality, and potentially identifies a multipotent pancreas progenitor cell. Genesis (New York, N.Y. : 2000). 30(3):137-140

Farber, S.A., Pack, M., Ho, S.Y., Johnson, I.D., Wagner, D.S., Dosch, R., Mullins, M.C., Hendrickson, H.S., Hendrickson, E.K., and Halpern, M.E. (2001) Genetic analysis of digestive physiology using fluorescent phospholipid reporters. Science (New York, N.Y.). 292(5520):1385-1388

Grosser, T., Cheskis, E., Pack, M.A., and FitzGerald, G.A. (2001) Comparative characterization of zebrafish cyclooxygenase isozymes: Cloning, function and expression. Arterioscler. Thromb. Vasc. Biol.. 21(4):191

Ho, S.Y., Pack, M., and Farber, S.A. (2001) Real-time imaging of lipid processing in wild type and mutant zebrafish. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 15(5):A1102

Grosser, T., Pack, M.A., and Fitzgerald, G.A. (2000) Cloning and characterization of zebrafish cyclooxygenase-2. Circulation. 102(18):1591

Pack, M., Solnica-Krezel, L., Malicki, J., Neuhauss, S.C., Schier, A-, F., Stemple, D.L., Driever, W., and Fishman, M.C. (1996) Mutations affecting development of zebrafish digestive organs. Development (Cambridge, England). 123:321-328

Abdelilah, S.,Driever, W., Gorn, A., Malicki,J., Neuhauss, S., Pack, M., Rangini, Z., Schier, A., Solnica-Krezel, L., Stanier, D., and Stemple, D. (1996) Anatomy of the 24, 48, 72 and 120 hours Zebrafish (Danio rerio) Embryo. ZFIN Direct Data Submission.

NON-ZEBRAFISH PUBLICATIONS