ZFIN ID: ZDB-PUB-150708-6
A conserved role of αA-crystallin in the development of the zebrafish embryonic lens
Zou, P., Wu, S.Y., Koteiche, H.A., Mishra, S., Levic, D.S., Knapik, E., Chen, W., Mchaourab, H.S.
Date: 2015
Source: Experimental Eye Research 138: 104-13 (Journal)
Registered Authors: Chen, Wenbiao, Knapik, Ela W., Wu, Shu-Yu (Simon)
Keywords: Alpha-crystallin, TALEN, cataract, chaperone, lens development, maternal transcript, morpholino, small heat shock protein, zebrafish
MeSH Terms: Animals; Animals, Genetically Modified; Blotting, Western; Electrophoresis, Polyacrylamide Gel; Embryo, Nonmammalian/physiology* (all 11) expand
PubMed: 26149094 Full text @ Exp. Eye. Res.
FIGURES   (current status)
ABSTRACT
αA- and αB-crystallins are small heat shock proteins that bind thermodynamically destabilized proteins thereby inhibiting their aggregation. Highly expressed in the mammalian lens, the α-crystallins have been postulated to play a critical role in the maintenance of lens optical properties by sequestering age-damaged proteins prone to aggregation as well as through a multitude of roles in lens epithelial cells. Here, we have examined the role of α-crystallins in the development of the vertebrate zebrafish lens. For this purpose, we have carried out morpholino-mediated knockdown of αA-, αBa- and αBb-crystallin and characterized the gross morphology of the lens. We observed lens abnormalities, including increased reflectance intensity, as a consequence of the interference with expression of these proteins. These abnormalities were less frequent in transgenic zebrafish embryos expressing rat αA-crystallin suggesting a specific role of α-crystallins in embryonic lens development. To extend and confirm these findings, we generated an αA-crystallin knockout zebrafish line. A more consistent and severe lens phenotype was evident in maternal/zygotic αA-crystallin mutants compared to those observed by morpholino knockdown. The penetrance of the lens phenotype was reduced by transgenic expression of rat αA-crystallin and its severity was attenuated by maternal αA-crystallin expression. These findings demonstrate that the role of α-crystallins in lens development is conserved from mammals to zebrafish and set the stage for using the embryonic lens as a model system to test mechanistic aspects of α-crystallin chaperone activity and to develop strategies to fine-tune protein-protein interactions in aging and cataracts.
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