PUBLICATION
Defining the phenotype of neutrophils following reverse migration in zebrafish
- Authors
- Ellett, F., Elks, P.M., Robertson, A.L., Ogryzko, N.V., Renshaw, S.A.
- ID
- ZDB-PUB-150614-1
- Date
- 2015
- Source
- Journal of Leukocyte Biology 98(6): 975-81 (Journal)
- Registered Authors
- Elks, Philip, Ellett, Felix, Ogryzko, Nikolay, Renshaw, Steve A., Robertson, Anne
- Keywords
- chemotaxis, granulocytes, infection, inflammation, phagocytosis
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/genetics
- Animals, Genetically Modified/immunology
- Cell Movement/genetics
- Cell Movement/immunology*
- Inflammation/genetics
- Inflammation/immunology
- Neutrophils/immunology*
- Staphylococcus aureus/immunology
- Zebrafish/genetics
- Zebrafish/immunology*
- PubMed
- 26071567 Full text @ J. Leukoc. Biol.
Citation
Ellett, F., Elks, P.M., Robertson, A.L., Ogryzko, N.V., Renshaw, S.A. (2015) Defining the phenotype of neutrophils following reverse migration in zebrafish. Journal of Leukocyte Biology. 98(6):975-81.
Abstract
Stimulation of neutrophil reverse migration presents an attractive, alternative therapeutic pathway to driving inflammation resolution. However, little is known about whether the activity of wound-experienced neutrophils is altered and whether encouraging dispersal of such neutrophils back into the body may have undesirable consequences. This study used a zebrafish tail transection inflammation model, in combination with a photoconvertible neutrophil transgenic line, to allow internally controlled, simultaneous comparison of reverse-migrated neutrophils with naïve neutrophils in the presence and absence of secondary insult. Detailed microscopy revealed that reverse-migrated neutrophils exhibited an activated morphology but responded normally to secondary insult and are able to mount an effective antimicrobial response to Staphylococcus aureus. These results support a model in which reverse-migrated neutrophils exhibit no long-term behavioral alterations and encourage the notion of enhanced reverse migration as a viable target for pharmaceutical manipulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping