|ZFIN ID: ZDB-PUB-120628-3|
Imaging vertebrate digestive function and lipid metabolism in vivo.
Otis, J.P., and Farber, S.A.
|Source:||Drug Discovery Today: Disease Models 10(1): e11-e16 (Review)|
|Registered Authors:||Farber, Steven, Otis, Jessica P.|
|PubMed:||24187571 Full text @ Drug Discov. Today Dis. Models|
Otis, J.P., and Farber, S.A. (2013) Imaging vertebrate digestive function and lipid metabolism in vivo.. Drug Discovery Today: Disease Models. 10(1):e11-e16.
ABSTRACTChallenges in imaging lipid-processing events in live, intact vertebrate models have historically led to reliance on cultured cell studies, thus hampering our understanding of lipid metabolism and gastrointestinal physiology. Fluorescently-labeled molecules, such as BODIPY-labeled lipids, can reveal lipid-processing events in live zebrafish (Danio rerio) and has expanded our understanding of digestive physiology. This review will cover recent advances from the past two to three years in the use of fluorescence-based imaging techniques in live zebrafish to characterize gastrointestinal physiology in health and disease and to conduct small molecule screens to discover therapeutic compounds.