ZFIN ID: ZDB-PUB-120125-14
Cloning and developmental expression of zebrafish pdzrn3
Dente, L., Gestri, G., Tsang, M., Kudoh, T., Wilson, S.W., Dawid, I.B., and Andreazzoli, M.
Date: 2011
Source: The International journal of developmental biology   55(10-12): 989-993 (Journal)
Registered Authors: Andreazzoli, Massimiliano, Dawid, Igor B., Gestri, Gaia, Kudoh, Tetsuhiro, Tsang, Michael, Wilson, Steve
Keywords: PDZRN protein, PDZ domain, RING finger, zebrafish development, ventral retina
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Carrier Proteins/biosynthesis
  • Carrier Proteins/genetics*
  • Cloning, Molecular
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental*
  • In Situ Hybridization
  • Molecular Sequence Data
  • Motor Neurons/metabolism
  • Mutation
  • Protein Structure, Tertiary
  • Retina/embryology
  • Retina/metabolism
  • Sequence Homology, Amino Acid
  • Thalamus/embryology
  • Time Factors
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics*
PubMed: 22252497 Full text @ Int. J. Dev. Biol.

Pdzrn3, a member of the PDZRN/SEMCAP/LNX protein family containing a RING finger and two PDZ domains, has been implicated in myoblast and osteoblast differentiation. However, its spatio-temporal expression pattern during embryonic development has not been defined. Here, we describe the cloning and expression pattern of pdzrn3 during zebrafish development. We found that in addition to being expressed in several mesodermal structures, this gene displays specific expression in the central nervous system including rhombomere 1, ventral retina, thalamus and motor neurons, indicating a novel function during neural development. In particular, the absence of expression of pdzrn3 in the ventral retina of noi mutant fish suggests a possible role for this gene in regulating fasciculation and/or navigation of retinal ganglion cell axons.