Mediator subunit 12 is required for neutrophil development in zebrafish
- Keightley, M.C., Layton, J.E., Hayman, J.W., Heath, J.K., and Lieschke, G.J.
- PLoS One 6(8): e23845 (Journal)
- Registered Authors
- Hayman, John W., Heath, Joan K., Keightley, M. Cristina, Layton, Judy E., Lieschke, Graham J.
- MeSH Terms
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Mediator Complex/genetics
- Mediator Complex/metabolism*
- Mutation, Missense/genetics
- Zebrafish/growth & development*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- 21901140 Full text @ PLoS One
Keightley, M.C., Layton, J.E., Hayman, J.W., Heath, J.K., and Lieschke, G.J. (2011) Mediator subunit 12 is required for neutrophil development in zebrafish. PLoS One. 6(8):e23845.
Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes