| ZFIN ID: ZDB-PUB-110914-17 |
Mediator subunit 12 is required for neutrophil development in zebrafish
Keightley, M.C., Layton, J.E., Hayman, J.W., Heath, J.K., and Lieschke, G.J.
| Date: | 2011 |
|---|---|
| Source: | PLoS One 6(8): e23845 (Journal) |
| Registered Authors: | Hayman, John W., Heath, Joan K., Keightley, M. Cristina, Layton, Judy E., Lieschke, Graham J. |
| Keywords: | none |
| MeSH Terms: |
|
| PubMed: | 21901140 Full text @ PLoS One |
Citation
Keightley, M.C., Layton, J.E., Hayman, J.W., Heath, J.K., and Lieschke, G.J. (2011) Mediator subunit 12 is required for neutrophil development in zebrafish. PLoS One. 6(8):e23845.
ABSTRACT
Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator.
ADDITIONAL INFORMATION
- Genes / Markers (19)
- Morpholino (1)
- Engineered Foreign Genes (2)
- Expression and Phenotype Data
- Mapping Details (4)
- Mutations and Transgenics (4)
- Fish (9)
- Orthology (2)