PUBLICATION
Mediator subunit 12 is required for neutrophil development in zebrafish
- Authors
- Keightley, M.C., Layton, J.E., Hayman, J.W., Heath, J.K., and Lieschke, G.J.
- ID
- ZDB-PUB-110914-17
- Date
- 2011
- Source
- PLoS One 6(8): e23845 (Journal)
- Registered Authors
- Hayman, John W., Heath, Joan K., Keightley, M. Cristina, Layton, Judy E., Lieschke, Graham J.
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Erythropoiesis/genetics
- Erythropoiesis/physiology
- Hematopoiesis/genetics
- Hematopoiesis/physiology
- Mediator Complex/genetics
- Mediator Complex/metabolism*
- Mutation, Missense/genetics
- Myelopoiesis/genetics
- Myelopoiesis/physiology
- Neutrophils/metabolism*
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 21901140 Full text @ PLoS One
Citation
Keightley, M.C., Layton, J.E., Hayman, J.W., Heath, J.K., and Lieschke, G.J. (2011) Mediator subunit 12 is required for neutrophil development in zebrafish. PLoS One. 6(8):e23845.
Abstract
Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping