ZFIN ID: ZDB-PUB-110914-17
Mediator subunit 12 is required for neutrophil development in zebrafish
Keightley, M.C., Layton, J.E., Hayman, J.W., Heath, J.K., and Lieschke, G.J.
Date: 2011
Source: PLoS One   6(8): e23845 (Journal)
Registered Authors: Hayman, John W., Heath, Joan K., Keightley, M. Cristina, Layton, Judy E., Lieschke, Graham J.
Keywords: none
MeSH Terms:
  • Animals
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Erythropoiesis/genetics
  • Erythropoiesis/physiology
  • Hematopoiesis/genetics
  • Hematopoiesis/physiology
  • Mediator Complex/genetics
  • Mediator Complex/metabolism*
  • Mutation, Missense/genetics
  • Myelopoiesis/genetics
  • Myelopoiesis/physiology
  • Neutrophils/metabolism*
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 21901140 Full text @ PLoS One

Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator.