ZFIN ID: ZDB-PERS-000912-4
Heath, Joan K.
Email: joan.heath@wehi.edu.au
URL: http://www.wehi.edu.au/faculty_members/associate_professor_joan_heath
Affiliation: Heath Lab
Address: The Walter and Eliza Hall Institute of Medical Research 1G Royal Parade | Parkville Melbourne | Vic 3052 | Australia
Country: Australia
Phone: (+613) 9345 2872
Fax: (+613) 9347 0852

Zebrafish intestinal development
Genetic regulation of colon cancer
RNA splicing
RNA binding proteins
Gene expression

Morgan, K.J., Doggett, K., Geng, F., Mieruszynski, S., Whitehead, L., Smith, K.A., Hogan, B.M., Simons, C., Baillie, G.J., Molania, R., Papenfuss, A.T., Hall, T.E., Ober, E.A., Stainier, D.Y.R., Gong, Z., Heath, J.K. (2023) ahctf1 and kras mutations combine to amplify oncogenic stress and restrict liver overgrowth in a zebrafish model of hepatocellular carcinoma. eLIFE. 12:
Koltowska, K., Okuda, K.S., Gloger, M., Rondon-Galeano, M., Mason, E., Xuan, J., Dudczig, S., Chen, H., Arnold, H., Skoczylas, R., Bower, N.I., Paterson, S., Lagendijk, A.K., Baillie, G.J., Leshchiner, I., Simons, C., Smith, K.A., Goessling, W., Heath, J.K., Pearson, R.B., Sanij, E., Schulte-Merker, S., Hogan, B.M. (2021) The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function. Nature cell biology. 23:1136-1147
Baell, J.B., Leaver, D.J., Hermans, S.J., Kelly, G.L., Brennan, M.S., Downer, N.L., Nguyen, N., Wichmann, J., McRae, H.M., Yang, Y., Cleary, B., Lagiakos, H.R., Mieruszynski, S., Pacini, G., Vanyai, H.K., Bergamasco, M.I., May, R.E., Davey, B.K., Morgan, K.J., Sealey, A.J., Wang, B., Zamudio, N., Wilcox, S., Garnham, A.L., Sheikh, B.N., Aubrey, B.J., Doggett, K., Chung, M.C., de Silva, M., Bentley, J., Pilling, P., Hattarki, M., Dolezal, O., Dennis, M.L., Falk, H., Ren, B., Charman, S.A., White, K.L., Rautela, J., Newbold, A., Hawkins, E.D., Johnstone, R.W., Huntington, N.D., Peat, T.S., Heath, J.K., Strasser, A., Parker, M.W., Smyth, G.K., Street, I.P., Monahan, B.J., Voss, A.K., Thomas, T. (2018) Inhibitors of histone acetyltransferases KAT6A/B induce senescence and arrest tumour growth. Nature. 560(7717):253-257
Miles, L.B., Darido, C., Kaslin, J., Heath, J.K., Jane, S.M., Dworkin, S. (2017) Mis-expression of grainyhead-like transcription factors in zebrafish leads to defects in enveloping layer (EVL) integrity, cellular morphogenesis and axial extension. Scientific Reports. 7:17607
Keightley, M.C., Carradice, D.P., Layton, J.E., Pase, L., Bertrand, J.Y., Wittig, J.G., Dakic, A., Badrock, A.P., Cole, N.J., Traver, D., Nutt, S.L., McCoey, J., Buckle, A.M., Heath, J.K., Lieschke, G.J. (2017) The Pu.1 target gene Zbtb11 regulates neutrophil development through its integrase-like HHCC zinc finger. Nature communications. 8:14911
Don, E.K., de Jong-Curtain, T.A., Doggett, K., Hall, T.E., Heng, B., Badrock, A.P., Winnick, C., Nicholson, G.A., Guillemin, G.J., Currie, P.D., Hesselson, D., Heath, J.K., Cole, N.J. (2016) Genetic basis of hindlimb loss in a naturally occurring vertebrate model. Biology Open. 5(3):359-66
Keightley, M.C., Markmiller, S., Love, C.G., Rasko, J.E., Lieschke, G.J., Heath, J.K. (2016) Experimental approaches to studying the nature and impact of splicing variation in zebrafish. Methods in cell biology. 135:259-88
Lobert, V.H., Mouradov, D., Heath, J.K. (2016) Focusing the Spotlight on the Zebrafish Intestine to Illuminate Mechanisms of Colorectal Cancer. Advances in experimental medicine and biology. 916:411-37
Dworkin, S., Simkin, J., Darido, C., Partridge, D.D., Georgy, S.R., Caddy, J., Wilanowski, T., Lieschke, G.J., Doggett, K., Heath, J.K., Jane, S.M. (2014) Grainyhead-like 3 regulation of endothelin-1 in the pharyngeal endoderm is critical for growth and development of the craniofacial skeleton. Mechanisms of Development. 133:77-90
Markmiller, S., Cloonan, N., Lardelli, R.M., Doggett, K., Keightley, M.C., Boglev, Y., Trotter, A.J., Ng, A.Y., Wilkins, S.J., Verkade, H., Ober, E.A., Field, H.A., Grimmond, S.M., Lieschke, G.J., Stainier, D.Y., and Heath, J.K. (2014) Minor class splicing shapes the zebrafish transcriptome during development. Proceedings of the National Academy of Sciences of the United States of America. 111(8):3062-3067
Keightley, M.C., Crowhurst, M.O., Layton, J.E., Beilharz, T., Markmiller, S., Varma, S., Hogan, B.M., de Jong-Curtain, T.A., Heath, J.K., and Lieschke, G.J. (2013) In vivo mutation of pre-mRNA processing factor 8 (Prpf8) affects transcript splicing, cell survival and myeloid differentiation. FEBS letters. 587(14):2150-2157
Boglev, Y., Badrock, A.P., Trotter, A.J., Du, Q., Richardson, E.J., Parslow, A.C., Markmiller, S.J., Hall, N.E., de Jong-Curtain, T.A., Ng, A.Y., Verkade, H., Ober, E.A., Field, H.A., Shin, D., Shin, C.H., Hannan, K.M., Hannan, R.D., Pearson, R.B., Kim, S.H., Ess, K.C., Lieschke, G.J., Stainier, D.Y., and Heath, J.K. (2013) Autophagy induction is a tor- and tp53-independent cell survival response in a zebrafish model of disrupted ribosome biogenesis. PLoS Genetics. 9(2):e1002379
Pase, L., Layton, J.E., Wittmann, C., Ellett, F., Nowell, C.J., Reyes-Aldasoro, C.C., Varma, S., Rogers, K.L., Hall, C.J., Keightley, M.C., Crosier, P.S., Grabher, C., Heath, J.K., Renshaw, S.A., and Lieschke, G.J. (2012) Neutrophil-Delivered Myeloperoxidase Dampens the Hydrogen Peroxide Burst after Tissue Wounding in Zebrafish. Current biology : CB. 22(19):1818-1824
Dworkin, S., Darido, C., Georgy, S.R., Wilanowski, T., Srivastava, S., Ellett, F., Pase, L., Han, Y., Meng, A., Heath, J.K., Lieschke, G.J., and Jane, S.M. (2012) Midbrain-hindbrain boundary patterning and morphogenesis are regulated by diverse grainy head-like 2-dependent pathways. Development (Cambridge, England). 139(3):525-536
Keightley, M.C., Layton, J.E., Hayman, J.W., Heath, J.K., and Lieschke, G.J. (2011) Mediator subunit 12 is required for neutrophil development in zebrafish. PLoS One. 6(8):e23845
Cerveny, K.L., Cavodeassi, F., Turner, K.J., de Jong-Curtain, T.A., Heath, J.K., and Wilson, S.W. (2010) The zebrafish flotte lotte mutant reveals that the local retinal environment promotes the differentiation of proliferating precursors emerging from their stem cell niche. Development (Cambridge, England). 137(13):2107-2115
de Jong-Curtain, T.A., Parslow, A.C., Trotter, A.J., Hall, N.E., Verkade, H., Tabone, T., Christie, E.L., Crowhurst, M.O., Layton, J.E., Shepherd, I.T., Nixon, S.J., Parton, R.G., Zon, L.I., Stainier, D.Y., Lieschke, G.J., and Heath, J.K. (2009) Abnormal nuclear pore formation triggers apoptosis in the intestinal epithelium of elys-deficient zebrafish. Gastroenterology. 136(3):902-911
Trotter, A.J., Parslow, A.C., and Heath, J.K. (2009) Morphologic analysis of the zebrafish digestive system. Methods in molecular biology (Clifton, N.J.). 546:289-315
Mizoguchi, T., Verkade, H., Heath, J.K., Kuroiwa, A., and Kikuchi, Y. (2008) Sdf1/Cxcr4 signaling controls the dorsal migration of endodermal cells during zebrafish gastrulation. Development (Cambridge, England). 135(15):2521-2529
Wilkins, S.J., Yoong, S., Verkade, H., Mizoguchi, T., Plowman, S.J., Hancock, J.F., Kikuchi, Y., Heath, J.K., and Perkins, A.C. (2008) Mtx2 directs zebrafish morphogenetic movements during epiboly by regulating microfilament formation. Developmental Biology. 314(1):12-22
Hogan, B.M., Verkade, H., Lieschke, G.J., and Heath, J.K. (2008) Manipulation of gene expression during zebrafish embryonic development using transient approaches. Methods in molecular biology (Clifton, N.J.). 469:273-300
Christie, E.L., Parslow, A.C., and Heath, J.K. (2008) Determination of mRNA and protein expression patterns in zebrafish. Methods in molecular biology (Clifton, N.J.). 469:253-272
Verkade, H., and Heath, J.K. (2008) Wnt signaling mediates diverse developmental processes in zebrafish. Methods in molecular biology (Clifton, N.J.). 469:225-251
Dworkin, S., Heath, J.K., Dejong-Curtain, T.A., Hogan, B.M., Lieschke, G.J., Malaterre, J., Ramsay, R.G., and Mantamadiotis, T. (2007) CREB activity modulates neural cell proliferation, midbrain-hindbrain organization and patterning in zebrafish. Developmental Biology. 307(1):127-141
Hogan, B.M., Layton, J.E., Pyati, U.J., Nutt, S.L., Hayman, J.W., Varma, S., Heath, J.K., Kimelman, D., and Lieschke, G.J. (2006) Specification of the Primitive Myeloid Precursor Pool Requires Signaling through Alk8 in Zebrafish. Current biology : CB. 16(5):506-511
Ng, A.N., de Jong-Curtain, T.A., Mawdsley, D.J., White, S.J., Shin, J., Appel, B., Dong, P.D., Stainier, D.Y., and Heath, J.K. (2005) Formation of the digestive system in zebrafish: III. Intestinal epithelium morphogenesis. Developmental Biology. 286(1):114-135
Hogan, B.M., Danks, J.A., Layton, J.E., Hall, N.E., Heath, J.K., and Lieschke, G.J. (2005) Duplicate zebrafish pth genes are expressed along the lateral line and in the central nervous system during embryogenesis. Endocrinology. 146(2):547-551
Hogan, B.M., Hunter, M.P., Oates, A.C., Crowhurst, M.O., Hall, N.E., Heath, J.K., Prince, V.E., and Lieschke, G.J. (2004) Zebrafish gcm2 is required for gill filament budding from pharyngeal ectoderm. Developmental Biology. 276(2):508-522
Mawdsley, D.J., Cooper, H.M., Hogan, B.M., Cody, S.H., Lieschke, G.J., and Heath, J.K. (2004) The Netrin receptor Neogenin is required for neural tube formation and somitogenesis in zebrafish. Developmental Biology. 269(1):302-315

Johnston APR, Kamphuis MMJ, Such GK, Scott AM, Nice EC, Heath JK, Caruso F (2012). Targeting cancer cells: controlling the binding and internalization of antibody-functionalized capsules. ACS Nano 6:6667-6674

Shimoni O, Postma A, Yan Y, Scott AM, Heath JK, Nice EC, Zelikin AN, Caruso F (2012). Macromolecule functionalization of redox-active hydrogel capsules and cancer cell targeting. ACS Nano 6:1463-1472

Yan Y, Wang Y, Heath JK, Nice EC, Caruso F. Cellular association and cargo release of redox-responsive polymer capsules mediated by exofacial thiols (2011). Adv Mater 23:3916-3921

Heath JK (2010) Transcriptional networks and signaling pathways that govern vertebrate intestinal development. Invited Review: In Current Topics in Developmental Biology Volume 90. Organogenesis in Development. (Ed. Peter Koopman, Elsevier) pp 159-192.

Kamphuis, MMJ, Johnston APR, Such GK, Dam HH, Evans RA, Scott AM, Nice EC, Heath JK and Caruso F (2010). Targeting of cancer cells using click-functionalized polymer capsules. J Am Chem Soc 132:15881-3

Yan Y, Ochs CJ, Such GK, Heath JK, Nice EC, Caruso F (2010). Bypassing multidrug resistance in cancer cells with biodegradable polymer capsules. Adv Mater. 22:5398-5403

Wang Y, Yan Y, Cui J, Hosta-Rigau L, Heath JK, Nice EC, Caruso F (2010). Encapsulation of water-insoluble drugs in polymer capsules prepared using mesoporous silica templates for intracellular drug delivery. Adv Mater 22(38):4293-4297

Yan Y, Johnston AP, Dodds SJ, Kamphuis MM, Ferguson C, Parton RG, Nice EC, Heath JK,* Caruso F* (2010). Uptake and intracellular fate of disulfide-bonded polymer hydrogel capsules for doxorubicin delivery to colorectal cancer cells. ACS Nano 4:2928-2936. *denotes equal contribution

Buchert M,* Athineos D,* Abud HE,* Burke ZD, Faux MC, Samuel MS, Jarnicki AG, Winbanks CE, Newton IP, Meniel VS, Suzuki H, Stacker SA, N�thke IS, Tosh D, Huelsken J, Clarke AR, Heath JK, Owen J. Sansom OJ, Ernst M (2010). Genetic dissection of differential signaling threshold requirements for the Wnt/β-catenin pathway in vivo. PLoS Genet. Jan 15;6(1):e1000816. *denotes equal contribution

Pereira-Fantini PM, Judd LM, Kalantzis A, Peterson AA, Ernst M, Heath JK and Giraud AS (2010). A33 antigen-deficient mice have defective colonic mucosal repair. Inflammatory Bowel Disease 16:604-12

Hosta-Rigau L, St�dler B, Yan Yan, Nice EC, Heath JK, Albericio F and Caruso F (2010). Capsosomes with multilayered subcompartments: assembly and loading with hydrophobic cargo. In press, Advanced Functional Materials 20:59-66

Samuel MS, Suzuki H,, Buchert M, Putoczki TL, Tebbutt NC, Lundgren-May T, Christou A, Inglese M, Toyota M, Heath JK, Ward RL, Waring PW and Ernst M (2009). Elevated Dnmt3a activity promotes polyposis in ApcMin mice by relaxing extracellular restraints on Wnt signaling. Gastroenterology 137:902-913

B�ning J, von Smolinski D, Tafazzoli K, Zimmer KP, Strobel S, Apostolaki M, Kollias G, Heath JK, Ludwig D, Gebert A (2008). Multivesicular bodies in intestinal epithelial cells: responsible for MHC class II-restricted antigen processing and origin of exosomes. Immunology 125: 510–521

Cortez C, Tomaskovic-Crook E, Johnston APR, Scott AM, Nice EC, Heath JK, Caruso F (2007). Influence of size, surface, cell line and kinetic properties on the specific binding of A33 antigen-targeted multilayered particles to colorectal cancer cells. ACS Nano 1:93–102

Malaterre J, Carpinelli M, Ernst M, Alexander W, Cooke M, Sutton S, Dworkin S, Heath JK, Frampton J, McArthur G, Clevers H, Hilton D, Mantamadiotis T, Ramsay RG 2007.
c-Myb is required for progenitor cell homeostasis in colonic crypts. Proc Natl Acad Sci USA 104:3829-3834

Lee JW, Epardaud M, Sun J, Becker JE, Cheng AC, Yonekura AR, Heath JK, Turley SJ 2007.
Peripheral antigen display by lymph node stroma promotes T cell tolerance to intestinal self. Nat Immunol. 2007 8:181-90

Cortez C, Tomaskovic-Crook E, Johnston A.P.R., Radt B, Cody S.H., Scott A.M., Nice E.C., Heath J.K., Caruso F 2006.
Targeting and uptake of multilayered particles to colorectal cancer cells. Advanced Materials 18: 1998-2003

Abud HE, Watson N, Heath JK 2005.
Growth of intestinal epithelium in organ culture is dependent on EGF signalling. Exp Cell Res 303:252-262

Cody SH, Xiang SD, Layton MJ, Handman E, Lam MH, Layton JE, Nice EC, Heath JK 2005.
A simple method allowing DIC imaging in conjunction with confocal microscopy. J Microsc. 217:265-74

Abud HE, Lock P, Heath JK 2004.
Efficient gene transfer into the epithelial cell layer of embryonic mouse intestine using low-voltage electroporation. Gastroenterology 126:1779-87

Abud HE, Heath JK 2004.
Detecting apoptosis during the formation of polarized intestinal epithelium in organ culture. Cell Death Differ11:788-9

Miller ME, Michaylira CZ, Simmons JG, Ney DM, Dahly EM, Heath JK, Lund PK 2004.
Suppressor of cytokine signaling-2: a growth hormone-inducible inhibitor of intestinal epithelial cell proliferation. Gastroenterology 127:570-81.

Ramsay RG, Micallef SJ, Williams B, Lightowler S, Vincan E, Heath JK, Mantamadiotis T, Bertoncello I 2004.
Colony-stimulating factor-1 promotes clonogenic growth of normal murine colonic crypt epithelial cells in vitro. J Interferon Cytokine Res 24:416-27