ZFIN ID: ZDB-PERS-100216-2
Keightley, M. Cristina
Email: c.keightley@latrobe.edu.au
URL: http://www.latrobe.edu.au
Affiliation: Keightley Lab
Address: Lab Head | Myeloid Development and Disease Laboratory Senior Lecturer | Dept of Pharmacy and Applied Science School of Molecular Science | La Trobe University PO Box 199, Bendigo, Victoria 3552
Country: Australia
Phone: +61 (0)3 5444 7143
ORCID ID: 0000-0001-8141-4069

Our laboratory studies the transcriptional regulation of myeloid development and disease using zebrafish and mouse models to discover and understand molecular pathways in haematopoietic development. Within this context, we examine the role of transcription factors and mRNA splicing regulators, employing a multi-disciplinary approach integrating molecular genetics, biochemistry, genomics, and proteomics.

Cao, H., Naik, S.H., Amann-Zalcenstein, D., Hickey, P., Salim, A., Cao, B., Nilsson, S.K., Keightley, M.C., Lieschke, G.J. (2023) Late fetal hematopoietic failure results from ZBTB11 deficiency despite abundant HSC specification. Blood advances. 7(21):6506-6519
Isles, H.M., Loynes, C.A., Alasmari, S., Kon, F.C., Henry, K.M., Kadochnikova, A., Hales, J., Muir, C.F., Keightley, M.C., Kadirkamanathan, V., Hamilton, N., Lieschke, G.J., Renshaw, S.A., Elks, P.M. (2021) Pioneer neutrophils release chromatin within in vivo swarms. eLIFE. 10:
Manley, H.R., Potter, D.L., Heddleston, J.M., Chew, T.L., Keightley, M.C., Lieschke, G.J. (2020) Frontline Science: Dynamic cellular and subcellular features of migrating leukocytes revealed by in vivo lattice lightsheet microscopy. Journal of Leukocyte Biology. 108(2):455-468
Mathiyalagan, N., Miles, L.B., Anderson, P.J., Wilanowski, T., Grills, B.L., McDonald, S.J., Keightley, M.C., Charzynska, A., Dabrowski, M., Dworkin, S. (2019) Meta-Analysis of Grainyhead-Like Dependent Transcriptional Networks: A Roadmap for Identifying Novel Conserved Genetic Pathways. Genes. 10(11)
Schmitz, U., Pinello, N., Jia, F., Alasmari, S., Ritchie, W., Keightley, M.C., Shini, S., Lieschke, G., Wong, J., Rasko, J. (2017) Intron retention enhances gene regulatory complexity in vertebrates. Genome biology. 18:216
Keightley, M.C., Carradice, D.P., Layton, J.E., Pase, L., Bertrand, J.Y., Wittig, J.G., Dakic, A., Badrock, A.P., Cole, N.J., Traver, D., Nutt, S.L., McCoey, J., Buckle, A.M., Heath, J.K., Lieschke, G.J. (2017) The Pu.1 target gene Zbtb11 regulates neutrophil development through its integrase-like HHCC zinc finger. Nature communications. 8:14911
Pazhakh, V., Clark, S., Keightley, M.C., Lieschke, G.J. (2017) A GCSFR/CSF3R zebrafish mutant models the persistent basal neutrophil deficiency of severe congenital neutropenia. Scientific Reports. 7:44455
Keightley, M.C., Markmiller, S., Love, C.G., Rasko, J.E., Lieschke, G.J., Heath, J.K. (2016) Experimental approaches to studying the nature and impact of splicing variation in zebrafish. Methods in cell biology. 135:259-88
Keightley, M.C., Wang, C.H., Pazhakh, V., Lieschke, G.J. (2014) Delineating the roles of neutrophils and macrophages in zebrafish regeneration models. The international journal of biochemistry & cell biology. 56C:92-106
Markmiller, S., Cloonan, N., Lardelli, R.M., Doggett, K., Keightley, M.C., Boglev, Y., Trotter, A.J., Ng, A.Y., Wilkins, S.J., Verkade, H., Ober, E.A., Field, H.A., Grimmond, S.M., Lieschke, G.J., Stainier, D.Y., and Heath, J.K. (2014) Minor class splicing shapes the zebrafish transcriptome during development. Proceedings of the National Academy of Sciences of the United States of America. 111(8):3062-3067
Keightley, M.C., Crowhurst, M.O., Layton, J.E., Beilharz, T., Markmiller, S., Varma, S., Hogan, B.M., de Jong-Curtain, T.A., Heath, J.K., and Lieschke, G.J. (2013) In vivo mutation of pre-mRNA processing factor 8 (Prpf8) affects transcript splicing, cell survival and myeloid differentiation. FEBS letters. 587(14):2150-2157
Pase, L., Layton, J.E., Wittmann, C., Ellett, F., Nowell, C.J., Reyes-Aldasoro, C.C., Varma, S., Rogers, K.L., Hall, C.J., Keightley, M.C., Crosier, P.S., Grabher, C., Heath, J.K., Renshaw, S.A., and Lieschke, G.J. (2012) Neutrophil-Delivered Myeloperoxidase Dampens the Hydrogen Peroxide Burst after Tissue Wounding in Zebrafish. Current biology : CB. 22(19):1818-1824
Keightley, M.C., Layton, J.E., Hayman, J.W., Heath, J.K., and Lieschke, G.J. (2011) Mediator subunit 12 is required for neutrophil development in zebrafish. PLoS One. 6(8):e23845