PUBLICATION
Analysis of cilia structure and function in zebrafish
- Authors
- Malicki, J., Avanesov, A., Li, J., Yuan, S., and Sun, Z.
- ID
- ZDB-PUB-110520-28
- Date
- 2011
- Source
- Methods (San Diego, Calif.) 101: 39-74 (Chapter)
- Registered Authors
- Avanesov, Andrei S., Malicki, Jarema, Sun, Zhaoxia, Yuan, Shiaulou
- Keywords
- basal bodies, cilia, cryosection, photoreceptor cell, sensory neurons, whole-mount staining
- MeSH Terms
-
- Animals
- Cilia/physiology*
- Humans
- Zebrafish/embryology*
- Zebrafish/physiology
- PubMed
- 21550439 Full text @ Methods
Citation
Malicki, J., Avanesov, A., Li, J., Yuan, S., and Sun, Z. (2011) Analysis of cilia structure and function in zebrafish. Methods (San Diego, Calif.). 101:39-74.
Abstract
The cilium, a previously little studied cell surface protrusion, has emerged as an important organelle in vertebrate cells. This tiny structure is essential for normal embryonic development, including the formation of left-right asymmetry, limb morphogenesis, and the differentiation of sensory cells. In the adult, cilia also function in a variety of processes, such as the survival of photoreceptor cells, and the homeostasis in several tissues, including the epithelia of nephric ducts. Human ciliary malfunction is associated with situs inversus, kidney cysts, polydactyly, blindness, mental retardation, obesity, and many other abnormalities. The genetic accessibility and optical transparency of the zebrafish make it an excellent vertebrate model system to study cilia biology. In this chapter, we describe the morphology and distribution of cilia in zebrafish embryonic and larval organs. We also provide essential protocols to analyze cilia formation and function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping