ZFIN ID: ZDB-PUB-100719-23
JunB-CBF{beta} signaling is essential to maintain sarcomeric Z-disc structure and when defective leads to heart failure
Meder, B., Just, S., Vogel, B., Rudloff, J., Gärtner, L., Dahme, T., Huttner, I., Zankl, A., Katus, H.A., and Rottbauer, W.
Date: 2010
Source: Journal of Cell Science   123(Pt 15): 2613-2620 (Journal)
Registered Authors: Dahme, Tillmann, Just, Steffen, Meder, Benjamin, Rottbauer, Wolfgang, Rudloff, Jessica, Vogel, Britta
Keywords: CBFβ, Z-disc, Heart failure, Zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Core Binding Factor beta Subunit/chemistry
  • Core Binding Factor beta Subunit/genetics
  • Core Binding Factor beta Subunit/metabolism*
  • Heart Failure/genetics
  • Heart Failure/metabolism
  • Humans
  • Immunoblotting
  • In Situ Hybridization
  • Mice
  • Microscopy, Electron, Transmission
  • Molecular Sequence Data
  • Muscle, Skeletal/metabolism
  • Muscle, Skeletal/ultrastructure
  • Myocardium/metabolism
  • Myocardium/ultrastructure
  • Myocytes, Cardiac/metabolism
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins c-jun/genetics
  • Proto-Oncogene Proteins c-jun/metabolism*
  • Sarcomeres/genetics
  • Sarcomeres/metabolism*
  • Sequence Homology, Amino Acid
  • Zebrafish
PubMed: 20605922 Full text @ J. Cell Sci.
FIGURES
ABSTRACT
In muscle cells, a complex network of Z-disc proteins allows proper reception, transduction and transmission of mechanical and biochemical signals. Mutations in genes encoding different Z-disc proteins such as integrin-linked kinase (ILK) and nexilin have recently been shown to cause heart failure by distinct mechanisms such as disturbed mechanosensing, altered mechanotransduction or mechanical Z-disc destabilization. We identified core-binding factor beta (CBFbeta) as an essential component for maintaining sarcomeric Z-disc and myofilament organization in heart and skeletal muscle. In CBFbeta-deficient cardiomyocytes and skeletal-muscle cells, myofilaments are thinned and Z-discs are misaligned, leading to progressive impairment of heart and skeletal-muscle function. Transcription of the gene encoding CBFbeta mainly depends on JunB activity. In JunB-morphant zebrafish, which show a heart-failure phenotype similar to that of CBFbeta-deficient zebrafish, transcript and protein levels of CBFbeta are severely reduced. Accordingly, ectopic expression of CBFbeta can reconstitute cardiomyocyte function and rescue heart failure in JunB morphants, demonstrating for the first time an essential role of JunB-CBFbeta signaling for maintaining sarcomere architecture and function.
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