Differential effects of thin and thick filament disruption on zebrafish smooth muscle regulatory proteins
- Davuluri, G., Seiler, C., Abrams, J., Soriano, A.J., and Pack, M.
- Neurogastroenterology and motility 22(10): 1100-e285 (Journal)
- Registered Authors
- Pack, Michael, Seiler, Christoph
- actin binding proteins, enteric nervous system, peristalsis, smooth muscle, zebrafish
- MeSH Terms
- Actin Cytoskeleton/physiology*
- Calmodulin-Binding Proteins/biosynthesis
- Calmodulin-Binding Proteins/genetics
- Enteric Nervous System/physiology
- Microfilament Proteins/metabolism
- Microscopy, Confocal
- Muscle Proteins/metabolism*
- Muscle, Smooth/cytology*
- Muscle, Smooth/metabolism
- Muscle, Smooth/physiology*
- Myosin Heavy Chains/biosynthesis
- Myosin Heavy Chains/genetics
- Myosin Light Chains/biosynthesis
- Myosin Light Chains/genetics
- RNA, Antisense
- RNA, Messenger/biosynthesis
- RNA, Messenger/genetics
- Zebrafish Proteins/metabolism*
- 20591105 Full text @ Neurogastroenterol. Motil.
Davuluri, G., Seiler, C., Abrams, J., Soriano, A.J., and Pack, M. (2010) Differential effects of thin and thick filament disruption on zebrafish smooth muscle regulatory proteins. Neurogastroenterology and motility. 22(10):1100-e285.
Background The smooth muscle actin binding proteins Caldesmon and Tropomyosin (Tm) promote thin filament assembly by stabilizing actin polymerization, however, whether filament assembly affects either the stability or activation of these and other smooth muscle regulatory proteins is not known. Methods Measurement of smooth muscle regulatory protein levels in wild type zebrafish larvae following antisense knockdown of smooth muscle actin (Acta2) and myosin heavy chain (Myh11) proteins, and in colourless mutants that lack enteric nerves. Comparison of intestinal peristalsis in wild type and colourless larvae. Key Results Knockdown of Acta2 led to reduced levels of phospho-Caldesmon and Tm. Total Caldesmon and phospho-myosin light chain (p-Mlc) levels were unaffected. Knockdown of Myh11 had no effect on the levels of either of these proteins. Phospho-Caldesmon and p-Mlc levels were markedly reduced in colourless mutants that have intestinal motility comparable with wild type larvae. Conclusions & Inferences These in vivo findings provide new information regarding the activation and stability of smooth muscle regulatory proteins in zebrafish larvae and their role in intestinal peristalsis in this model organism.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes