ZFIN ID: ZDB-PUB-100330-43
Transgenic Expression of Walleye Dermal Sarcoma Virus rv-cyclin (orfA) in Zebrafish does not Result in Tissue Proliferation
Paul, T.A., Rovnak, J., Quackenbush, S.L., Whitlock, K., Zhan, H., Gong, Z., Spitsbergen, J., Bowser, P.R., and Casey, J.W.
Date: 2011
Source: Mar. Biotechnol. 13(2): 142-150 (Journal)
Registered Authors: Bowser, Paul R., Gong, Zhiyuan, Paul, Thomas A., Spitsbergen, Jan, Whitlock, Kate, Zhan, Huiqing
Keywords: Walleye dermal sarcoma virus, orfA, rv-cyclin, Fish retrovirus, Walleye dermal sarcoma, Zebrafish
MeSH Terms: Animals; Animals, Genetically Modified/metabolism*; Cell Proliferation; Epsilonretrovirus/genetics*; Fish Diseases/metabolism* (all 25) expand
PubMed: 20349325 Full text @ Mar. Biotechnol.
Walleye dermal sarcoma (WDS) is a benign tumor of walleye fish that develops and completely regresses seasonally. The retrovirus associated with this disease, walleye dermal sarcoma virus, encodes three accessory genes, two of which, rv-cyclin (orfA) and orfb, are thought to play a role in tumor development. In this study, we attempted to recapitulate WDS development by expressing rv-cyclin in chimeric and stable transgenic zebrafish. Six stable transgenic lines expressing rv-cyclin from the constitutive CMVtk promoter were generated. Immunohistochemistry and quantitative reverse transcriptase polymerase chain reaction demonstrate that rv-cyclin is widely expressed in different tissues in these fish. These lines were viable and histologically normal for up to 2 years. No increase in tumors or tissue proliferation was observed following N-ethyl N-nitrosourea exposure or following tail wounding and subsequent tissue regeneration compared to controls. These data indicate that rv-cyclin is not independently sufficient for tumor induction in zebrafish.