ZFIN ID: ZDB-PERS-971201-1
||Center for Fish Disease Research
and Marine and Freshwater Biomedical Sciences Center
220 Nash Hall
Oregon State University
BIOGRAPHY AND RESEARCH INTERESTS
I am a fisheries scientist and veterinary pathologist (Diplomate, American College of Veterinary Pathologists) who has conducted laboratory research and field epidemiologic studies in fish pathology and toxicology for over 25 years. My research has included studies of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxicology and pathologic lesions in a variety of fish species, studies of interactions of halogenated aromatic hydrocarbons with disease resistance and immune responses of salmonids, early life stage toxicity of TCDD and PCBs in salmonids, effects of TCDD on sexual development, fecundity and fertiliy in zebrafish, nutritional pathology, and field studies investigating the causes of tumor epizootics in brown bullhead, white suckers and lake trout. My current research focuses primarily on investigation of genetic influences on susceptibility of zebrafish to spontaneous and carcinogen-induced tumors. My collaborators and I are developing lines of zebrafish highly sensitive to carcinogen-induced neoplasia. In collaboration with Dr. John Postlethwait at the University of Oregon, I am deriving lines of zebrafish with inactivating mutations in homologs of human tumor suppressor genes. I am completing studies to evaluate the spontaneous rates of neoplasia in wild-type and mutant lines of zebrafish and investigate the relative roles of diet, husbandry systems, infectious agents, and genetic influences on rates of spontaneous neoplasia in various lines of zebrafish. I have collaborated with Dr. Donald Buhler of Oregon State University to clarify the roles of various cytochrome P450 enzymes in the carcinogenesis processes in zebrafish. To better understand mechanisms of carcinogenesis using the zebrafish model, I am working together with Dr. Zhiyuan Gong of the National University of Singapore to compare gene expression in normal and neoplastic liver using microarray technology. I am collaborating with molecular genetecist Dr. Istvan Magyary and with M.D. pathologist Dr. George Szekeres from Hungary to better characterize the expression of various proteins in normal and neoplastic tissues from zebrafish, with the goal of being better able to make definitive histologic diagnoses for neoplasms whose histologic characteristics make them difficult to classify. I am providing histopathologic expertise in collaborative studies with Dr. Wenbiao Chen of the Oregon Health and Science University in Portland OR in order to develop trangenic models of zebrafish for study of epidermal skin neoplasia and melanoma. I taught finfish histology, histopathology and neoplasia for 7 years in the Aquavet I and II programs in Woods Hole, MA, while on the faculty of the College of Veterinary Medicine at Cornell University. Together with Drs. Paul Bowser of Cornell and Mike Kent of Oregon State University, I teach a short course on Health and Colony Management of Laboratory Fish held at the Mount Desert Biological Laboratory in Maine. I have a strong interest in using cutting edge imaging techniques like micro-computerized axial tomography to characterize lesions such as neoplasms in zebrafish.
Grzelak, A.K., Davis, D.J., Caraker, S.M., Crim, M.J., Spitsbergen, J.M., Wiedmeyer, C.E. (2017) Stress Leukogram Induced by Acute and Chronic Stress in Zebrafish (Danio rerio). Comparative medicine. 67(4):263-269
Ju, B., Chen, W., Orr, B.A., Spitsbergen, J.M., Jia, S., Eden, C.J., Henson, H.E., Taylor, M.R. (2015) Oncogenic KRAS promotes malignant brain tumors in zebrafish. Molecular Cancer. 14:18
Liu, Q., Spitsbergen, J.M., Cariou, R., Huang, C.Y., Jiang, N., Goetz, G., Hutz, R.J., Tonellato, P.J., Carvan, M.J. (2014) Histopathologic alterations associated with global gene expression due to chronic dietary TCDD exposure in juvenile zebrafish. PLoS One. 9:e100910
Chew, T.W., Liu, X.J., Liu, L., Spitsbergen, J.M., Gong, Z., and Low, B.C. (2014) Crosstalk of Ras and Rho: activation of RhoA abates Kras-induced liver tumorigenesis in transgenic zebrafish models. Oncogene. 33(21):2717-27
Ju, B., Chen, W., Spitsbergen, J.M., Lu, J., Vogel, P., Peters, J.L., Wang, Y.D., Orr, B.A., Wu, J., Henson, H.E., Jia, S., Parupalli, C., Taylor, M.R. (2014) Activation of Sonic hedgehog signaling in neural progenitor cells promotes glioma development in the zebrafish optic pathway. Oncogenesis. 3:e96
Li, Z., Zheng, W., Wang, Z., Zeng, Z., Zhan, H., Li, C., Zhou, L., Yan, C., Spitsbergen, J.M., and Gong, Z. (2013) An inducible Myc zebrafish liver tumor model revealed conserved Myc signatures with mammalian liver tumors. Disease models & mechanisms. 427(1):223-226
Li, Z., Huang, X., Zhan, H., Zeng, Z., Li, C., Spitsbergen, J.M., Meierjohann, S., Schartl, M., and Gong, Z. (2012) Inducible and repressable oncogene-addicted hepatocellular carcinoma in Tet-on xmrk transgenic zebrafish. Journal of hepatology. 56(2):419-425
Nguyen, A.T., Emelyanov, A., Koh, C.H., Spitsbergen, J.M., Parinov, S., and Gong, Z. (2012) An inducible krasV12 transgenic zebrafish model for liver tumorigenesis and chemical drug screening. Disease models & mechanisms. 5(1):63-72
Nguyen, A.T., Emelyanov, A., Koh, C.H., Spitsbergen, J.M., Lam, S.H., Mathavan, S., Parinov, S., and Gong, Z. (2011) A high level of liver-specific expression of oncogenic KrasV12 drives robust liver tumorigenesis in transgenic zebrafish. Disease models & mechanisms. 4(6):801-13
Paul, T.A., Rovnak, J., Quackenbush, S.L., Whitlock, K., Zhan, H., Gong, Z., Spitsbergen, J., Bowser, P.R., and Casey, J.W. (2011) Transgenic Expression of Walleye Dermal Sarcoma Virus rv-cyclin (orfA) in Zebrafish does not Result in Tissue Proliferation. Mar. Biotechnol.. 13(2):142-150
Rodriguez-Mari, A., Wilson, C., Titus, T.A., Cañestro, C., Bremiller, R.A., Yan, Y.L., Nanda, I., Johnston, A., Kanki, J.P., Gray, E.M., He, X., Spitsbergen, J., Schindler, D., and Postlethwait, J.H. (2011) Roles of brca2 (fancd1) in Oocyte Nuclear Architecture, Gametogenesis, Gonad Tumors, and Genome Stability in Zebrafish. PLoS Genetics. 7(3):e1001357
Balla, K.M., Lugo-Villarino, G., Spitsbergen, J.M., Stachura, D.L., Hu, Y., Banuelos, K., Romo-Fewell, O., Aroian, R.V., and Traver, D. (2010) Eosinophils in the zebrafish: prospective isolation, characterization, and eosinophilia induction by helminth determinants. Blood. 116(19):3944-3954
Zhan, H., Spitsbergen, J.M., Qing, W., Wu, Y.L., Paul, T.A., Casey, J.W., Her, G.M., and Gong, Z. (2010) Transgenic Expression of Walleye Dermal Sarcoma Virus rv-cyclin Gene in Zebrafish and Its Suppressive Effect on Liver Tumor Development After Carcinogen Treatment. Mar. Biotechnol.. 12(6):640-649
King Heiden, T.C., Spitsbergen, J., Heideman, W., and Peterson, R.E. (2009) Persistent Adverse Effects on Health and Reproduction Caused by Exposure of Zebrafish to 2,3,7,8-Tetrachlorodibenzo-p-dioxin During Early Development and Gonad Differentiation. Toxicological sciences : an official journal of the Society of Toxicology. 109(1):75-87
Kent, M.L., Feist, S.W., Harper, C., Hoogstraten-Miller, S., Law, J.M., Sánchez-Morgado, J.M., Tanguay, R.L., Sanders, G.E., Spitsbergen, J.M., Whipps, C.M. (2009) Recommendations for control of pathogens and infectious diseases in fish research facilities. Comparative biochemistry and physiology. 149(2):240-248
Lam, S.H., Winata, C.L., Tong, Y., Korzh, S., Lim, W.S., Korzh, V., Spitsbergen, J., Mathavan, S., Miller, L.D., Liu, E.T., and Gong, Z. (2006) Transcriptome Kinetics of Arsenic-induced Adaptive Response in Zebrafish Liver. Physiological Genomics. 27(3):351-361
Lam, S.H., Wu, Y.L., Vega, V.B., Miller, L.D., Spitsbergen, J., Tong, Y., Zhan, H., Govindarajan, K.R., Lee, S., Mathavan, S., Krishna Murthy, K.R., Buhler, D.R., Liu, E.T., and Gong, Z. (2006) Conservation of gene expression signatures between zebrafish and human liver tumors and tumor progression. Nat. Biotechnol.. 24(1):73-75
Shepard, J.L., Amatruda, J.F., Stern, H.M., Subramanian, A., Finkelstein, D., Ziai, J., Finley, K.R., Pfaff, K.L., Hersey, C., Zhou, Y., Barut, B., Freedman, M., Lee, C., Spitsbergen, J., Neuberg, D., Weber, G., Golub, T.R., Glickman, J.N., Kutok, J.L., Aster, J.C., and Zon, L.I. (2005) A zebrafish bmyb mutation causes genome instability and increased cancer susceptibility. Proc. Natl. Acad. Sci. USA. 102(37):13194-13199
Andreasen, E.A., Spitsbergen, J.M., Tanguay, R.L., Stegeman, J.J., Heideman, W., and Peterson, R.E. (2002) Tissue-specific expression of AHR2, ARNT2, and CYP1A in zebrafish embryos and larvae: effects of developmental stage and 2,3,7,8-Tetrachlorodibenzo-p-dioxin exposure. Toxicological sciences : an official journal of the Society of Toxicology. 68(2):403-419
Spitsbergen, J.M., Tsai, H.W., Reddy, A., Miller, T., Arbogast, D., Hendricks, J.D., and Bailey, G.S. (2000) Neoplasia in zebrafish (Danio rerio) treated with N-methyl-N'-nitro-N-nitrosoguanidine by three exposure routes at different developmental stages. Toxicologic pathology. 28(5):716-725
Spitsbergen, J.M., Tsai, H.W., Reddy, A., Miller, T., Arbogast, D., Hendricks, J.D., and Bailey, G.S. (2000) Neoplasia in zebrafish (Danio rerio) treated with 7,12-dimethylbenz[a]anthracene by two exposure routes at different developmental stages. Toxicologic pathology. 28(5):705-715
Henry, T.R., Spitsbergen, J.M., Hornung, M.W., Abnet, C.C., and Peterson, R.E. (1997) Early life stage toxicity of 2,3,7,8-Tetrachlorodibenzo-p-dioxin in zebrafish (Danio rerio). Tox. App. Pharmacol.. 142(1):56-68
Spitsbergen, J.M., and M.J. Wolfe. 1995. The riddle of hepatic neoplasia in brown bullheads from relatively unpolluted waters in New York State. Toxicologic Pathology 23:716-725.
Spitsbergen, J.M., and M.J. Wolfe. 1995. Hepatocyte clusters in the spleen--a normal feature of some populations of brown bullheads in New York State. Toxicologic Pathology 23:726-730.
Spitsbergen, J.M., M.K. Walker, R.E. Peterson and J.R. Olson. 1991. Pathologic alterations in early life stages of lake trout exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Aquatic Toxicology 19:41-72.
Spitsbergen, J.M., J.M. Kleeman and R.E. Peterson. 1988. Morphologic lesions and acute toxicity in rainbow trout (Salmo gairdneri) treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Journal of Toxicology and Environmental Health 23:333-358.
Spitsbergen, J.M., J.M. Kleeman, K.A. Schat and R.E. Peterson. 1988. Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or Aroclor 1254 on the resistance of rainbow trout, Salmo gairdneri Richardson, to infectious hematopoietic necrosis virus. Journal of Fish Diseases 11:73-83.
Spitsbergen, J.M., J.M. Kleeman and R.E. Peterson. 1988. 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity in yellow perch Perca flavescens. Journal of Toxicology and Environmental Health 23:359-383.
Spitsbergen, J.M., and K.A. Schat. 1986. A chromium release assay for the assessment of spontaneous and immune hemolysis of erythrocytes by sera of rainbow trout. Developmental and Comparative Immunology 10:11-23.
Spitsbergen, J.M., K.A. Schat, J.M. Kleeman and R.E. Peterson. 1986. Interactions of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) with immune responses of rainbow trout. Veterinary Immunology and Immunopathology 12:263-280.
Kent, M.L., Watral, V.G., Whipps, C.M., Cunningham, M.E., Criscione, C.D., Heidel, J.R., Curtis, L.R., Spitsbergen, J.M., Markle, D.F. 2004. A digenean metacercaria (Apophallus sp.) and myxozoan (Myxobolus sp.) associated with vertebral deformities in cyprinid fishes from the Willamette River, Oregon. J. Aquat. An. Health. 16: 116-129.
Carlson, D.B., Williams, D.E., Spitsbergen, J.M., Ross, P.F., Bacon, C.W., Meredith, F.I., and Riley, R.T. 2001. Fumonisin B1 promotes aflatoxin B1 and N-methyl-N’-nitro-N-nitrosoguanidine initiated liver tumors in rainbow trout. Toxicol. Appl. Pharmacol. 172 (1):29-36.
Riley, R.T., Enongene, E., Voss, K.A., Norred, W.P., Meredith, F.I., Sharma, R.P., Spitsbergen, J.,Williams, D.E., Carlson, D.B., Merrill, A.H. Jr. 2001. Sphingolipid perturbations as mechanisms for fumonisin carcinogenesis. Environ. Health Perspect. 109 Suppl 2:301-8.
Foster, E.P., Fitzpatrick, M.S., Feist, G.W., Schreck, C.B., Yates, J., Spitsbergen, J.M., Heidel, J. 2001. Plasma androgen correlation, EROD induction, reduced condition factor, and the occurrence of organochlorine pollutants in reproductively immature white sturgeon (Acipenser transmontanus) from the Columbia River, USA. Arch. Environ. Contam. Toxicol. 41:182-91.
Guiney, P.D., Walker, M.K., Spitsbergen, J.M. and Peterson, R.E. 2000. Hemodynamic dysfunction and cytochrome P450 mRNA expression induced by TCDD during embryonic stages of lake trout development. Toxicol. Appl. Pharmacol. 168:1-14.
Diawara, M.M., Williams, D.E., Oganesian, A. and Spitsbergen, J.M. 2000. Dietary psoralens induce hepatotoxicity in C57 mice. Journal of Natural Toxins 9:179-195.
Reddy, A., Spitsbergen, J.M., Mathews, C., Hendricks,J.D. and Bailey, G.S. 1999. Hepatic tumorigenicity by dietary dibenzo[a,l]pyrene in medaka (Oryzias latipes). J. Environ. Pathol. Toxicol. 18(4):261-269.
Hornung, M.W., Spitsbergen, J.M. and Peterson, R.E. 1999. 2,3,7,8-Tetrachlorodibenzo-p-dioxin alters cardiovascular and craniofacial development and function in sac fry of rainbow trout (Oncorhynchus mykiss). Toxicol. Sci. 47:40-51.
Poulet, F.M. and J.M. Spitsbergen. 1996. Ultrastructural study of spontaneous orocutaneous neoplasms of brown bullheads (Ictalurus nebulosus). Diseases of Aquatic Organisms 24:93-98.
Fisher, J.P., J.M. Spitsbergen, T. Iamonte, E.E. Little, and A. DeLonay. 1995. Pathological and behavioral manifestations of the “Cayuga Syndrome”, a thiamine deficiency in larval landlocked Atlantic salmon. Journal of Aquatic Animal Health 7:269-283.
Fisher, J.P., J.M. Spitsbergen, R. Getchell, J.Symula, J. Skea, M. Babenzein and T. Chiotti. 1995. Reproductive failure in landlocked Atlantic salmon from New York’s Finger Lakes: investigations into the etiology and epidemiology of the “Cayuga Syndrome”. Journal of Aquatic Animal Health 7:81-94.
Fisher, J.P., Fitzsimons, J.D., Combs, G.F. and J.M. Spitsbergen. 1995. Naturally occurring thiamine deficiency causing reproductive failure in Finger Lakes Atlantic salmon. Trans. Am. Fish. Soc. 125: 167-178.
Fisher, J.P., J.M. Spitsbergen and B. Jahan-Parwar. 1994. Effects of embryonic PCB exposure on hatching success, survival, growth and developmental behavior in landlocked Atlantic salmon, Salmo salar. In: Environmental toxicology and risk assessment, 2nd Vol. ASTM STP 1173, eds. J.W. Gorsuch, F.J. Dwyer, C.G. Ingersoll and T.W. La Point, American Society for Testing and Materials, Philadelphia, PA. p. 298-314.
Poulet, F.M., M.J. Wolfe and J.M. Spitsbergen. 1994. Naturally occurring orocutaneous papillomas and carcinomas of brown bullheads (Ictalurus nebulosus) in New York State. Veterinary Pathology 31:8-18.
Poulet, F.M., Casey, J.W. and J.M. Spitsbergen. 1993. Studies on transmissibility and etiology of orocutaneous tumors of brown bullheads (Ictalurus nebulosus). Diseases of Aquatic Organisms 16:97-104.
Cheng, Li-Lin, P.R. Bowser and J.M. Spitsbergen. 1993. Development of cell cultures derived from lake trout liver and kidney in a hormone-supplemented, serum-reduced medium. Journal of Aquatic Animal Health 5:119-126.
Walker, M.K., J.M. Spitsbergen, J.R. Olson and R.E. Peterson. 1991. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxicity during early life stage development of lake trout (Salvelinus namaycush). Canadian Journal of Fisheries and Aquatic Sciences 48:875-883.
King Heiden, T.C., Spitsbergen, J.M., Heideman, W., Peterson, R.E. 2007. Early life stage dioxin exposure alters craniofacial development, induces cardiac toxicity, and impairs reproductive capability of adult zebrafish. Future Research on Endocrine Disruption: Translation of Basic and Animal Research to Understand Human Disease, Sponsored by the National Institutes of Environmental Health Sciences, Durham, NC.
King Heiden, T.C., Spitsbergen, J.M., Heideman, W., Peterson, R.E. 2007. Exposure to TCDD during embryonic development and gonad differentiation impairs reproductive capacity of zebrafish. Annual Meeting Society of Environmental Toxicology and Chemistry, Milwaukee, WI.
Spitsbergen, J.M., Norred, R., Taylor, A., Buhler, D.R. 2006. Lines of Zebrafish with Mutations in the uma Gene and in the Delta-Notch Signaling Pathway Show Elevated Incidences of Ultimobranchial Neoplasia Following Fry Immersion Treatment with the Polycyclic Aromatic Hydrocarbon Carcinogen 7,12-Dimethlybenz[a]anthracene (DMBA). Zebrafish Development and Genetics Meeting, Madison, WI.
Spitsbergen, J.M., Kent, M.L., Westerfield, M. 2005. Diet and husbandry system strongly influence tumor incidences in zebrafish of the AB line at two years of age. Aquatic Animal Models of Human Disease, Athens, GA.
Wang-Buhler, J., Chung, W., Tseng, H., Miranda, C., Hu, C., Hseu, T., Taylor, A., Spitsbergen, J.M., Buhler, D.R. 2005. Development of specific antipeptide antibodies against zebrafish xenobiotic metabolising forms of cytochrome P450. Society of Toxicology, New Orleans, LA.
Spitsbergen, J.M., Buhler, D.R., Miller, T. 2004. Another long fin and uma mutant lines are highly sensitive to polycyclic aromatic hydrocarbon-induced liver neoplasia. Zebrafish Development and Genetics Meeting, Madison, WI.
Bailey, G.S., Pratt, M.M., Pereira, C.B., Hendricks, J.D., Spitsbergen, J.M., Williams, D.E. 2003. Potential of the rainbow trout tumor model in cancer risk assessment. Aquatic Animal Models of Human Disease Conference, Manassas, VA.
Fournie, J.W., Hawkins, W.E., Vogelbein, W.K., Spitsbergen, J.M. 2003. Small fish species as models for exocrine pancreatic carcinogenesis. Aquatic Animal Models of Human Disease Conference, Manassas, VA.
Spitsbergen, J.M., Kent, M.L., Bishop-Stewart, J.K., Miller, T., Matthews, J.L., Buhler, D.R. 2002. Spontaneous and carcinogen-induced neoplasia and other lesions in wild-type and mutant lines of zebrafish. Zebrafish Development and Genetics Meeting, Madison, WI.
Jennifer L. Matthews, J.L., Spitsbergen, J., Bishop-Stewart, J., Westerfield, M., Kent, M.L. 2002. A summary of common diseases of laboratory zebrafish. Zebrafish Development and Genetics Meeting, Madison, WI.
Bishop-Stewart, J.K., Matthews, J.L. Larison, K., Spitsbergen, J., Westerfield, M., Kent, M.L. 2001. Diseases of Zebrafish in Research Facilities. Fish Health Section of American Fisheries Society. Victoria, British Columbia.
Beckwith, L.G., Moore, J.L. Tsao-Wu, G.S., Spitsbergen, J.M., Hendricks, J.D., Harshbarger J.C., Cheng, K.C. 2000. Induced and spontaneous neoplasia in zebrafish (Danio rerio). Cold Spring Harbor Zebrafish Development and Genetics Meeting.
Reddy, A., Spitsbergen, J.M., Hendricks, J. And Bailey, G. 1999. An ED0.1 dose-response tumor study of the environmental carcinogen dibenzo[a,l]pyrene (DB[a,l]P) in rainbow trout. Proc. Am. Assoc. Cancer Res. 40:405.
Spitsbergen, J.M., Tsai, H., Reddy, A. and Hendricks, J. 1997. Response of zebrafish to a panel of structurally diverse carcinogens. Proc. AACR 38, 354.
Oganesian, A., Siddens, L.K., Spitsbergen, J.M. and Williams, D.E. 1996. Induction of CYP1A1 in rat and rainbow trout liver slices using 12-well plates and the shaker system of incubation. Congress on In Vitro Biology, San Francisco.
Fisher, J.P., J.M. Spitsbergen, G.F. Combs, M. Babenzein and T. Chiotti. 1994. Reproductive failure in wild-caught Atlantic salmon Salmo salar from New York's Finger Lakes: Epizootiology of the Cayuga Syndrome. International Symposium on Aquatic Animal Health, Seattle, WA.
Fisher, J.P., J.M. Spitsbergen, T. Iamonte and B. Bush. 1994. Structure/activity neurotoxicity of PCBs to early-life-stages of the Atlantic salmon and rainbow trout: an ethological model. 18th Annual Larval Fish Conference, St. Andrews, New Brunswick.
Fisher, J.P., J.M. Spitsbergen, T. Reimers, S. Lamb, M. Babenzein and T. Chiotti. 1994. Epizootiology of the 'Cayuga Syndrome', a lethal condition of larval landlocked Atlantic salmon in New York's Finger Lakes. International Association for Great Lakes Research, Windsor, Ontario.
Fisher, J.P., J.M. Spitsbergen and B. Jahan-Parwar. 1992. Effects of embryonic PCB exposure on neurobehavioral development and growth in Atlantic salmon. Second Symposium on Environmental Toxicology and Risk Assessment. American Society for Testing and Materials, Philadelphia, PA.
Fisher, J.P., J.M. Spitsbergen, D.E. Rosen and B. Jahan-Parwar. 1990. Effects of embryonic PCB burdens on hatchability, survival, growth and developmental behavior in landlocked Atlantic salmon Salmo salar. Soc. Environ. Toxicol. Chem. 11th Ann. Mtg. Abstr.
Fisher, J.P. and J.M. Spitsbergen. 1990. Early life stage mortality syndrome in Cayuga Lake landlocked salmon. Roundtable on Contaminant-Caused Reproductive Problems in Salmonids, Windsor, Ontario.
Spitsbergen, J.M., P.R. Bowser and M.J. Wolfe. 1989. Epizootic neoplasia of the lateral line system of lake trout in New York's Finger Lakes. Fish Health Section of American Fisheries Society/Eastern Fish Health Workshop.
Spitsbergen, J.M., M.K. Walker, R.E. Peterson and J.R. Olson. 1988. Pathologic lesions in early life stages of lake trout exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Soc. Environ. Toxicol. Chem. 9th Ann. Mtg. Abstr.
Spitsbergen, J.M., and K.R. Cooper. 1988. Effects of various pharmacologic agents on the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to the medaka embryo. New Jersey Department of Environmental Protection/Environmental and Occupational Health Sciences Institute Annual Meeting, Trenton, NJ.
Spitsbergen, J.M., J.M. Kleeman and R.E. Peterson. 1984. Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in freshwater fish. Toxicologist 4:190.
Carlisle, J.C. and J. Spitsbergen. 1980. Bacterial kidney disease of rainbow trout in sea water. Biological Bulletin 159:495-496.