PUBLICATION

Zebrafish kidney stromal cell lines support multilineage hematopoiesis

Authors
Stachura, D.L., Reyes, J.R., Bartunek, P., Paw, B.H., Zon, L.I., and Traver, D.
ID
ZDB-PUB-090518-8
Date
2009
Source
Blood   114(2): 279-289 (Journal)
Registered Authors
Bartunek, Petr, Paw, Barry, Traver, David, Zon, Leonard I.
Keywords
none
MeSH Terms
  • Aging/physiology
  • Animals
  • Cell Line
  • Cell Lineage*
  • Cell Proliferation
  • Erythroid Cells/cytology
  • Erythroid Cells/drug effects
  • Erythropoietin/pharmacology
  • Gene Expression Regulation
  • Hematopoiesis*
  • Kidney/cytology*
  • Kidney/metabolism
  • Mutation/genetics
  • Stromal Cells/cytology*
  • Stromal Cells/metabolism
  • Zebrafish/embryology*
  • Zebrafish/metabolism
PubMed
19433857 Full text @ Blood
Abstract
Studies of zebrafish hematopoiesis have been largely performed using mutagenesis approaches and retrospective analyses based upon gene expression patterns in whole embryos. We previously developed transplantation assays to test the repopulation potentials of candidate hematopoietic progenitor cells. We have been impaired, however, in determining cellular differentiation potentials by a lack of short-term functional assays. To enable more precise analyses of hematopoietic progenitor cells, we have created zebrafish kidney stromal (ZKS) cell lines. Culture of adult whole kidney marrow with ZKS cells results in the maintenance and expansion of hematopoietic precursor cells. Hematopoietic growth is dependent upon ZKS cells, and we show that ZKS cells express many growth factors and ligands previously demonstrated to be important in maintaining mammalian hematopoietic cells. In the absence of exogenous growth factors, ZKS cells maintain early hematopoietic precursors and support differentiation of lymphoid and myeloid cells. With the addition of zebrafish erythropoietin, ZKS cells also support the differentiation of erythroid precursors. These conditions have enabled the ability to ascertain more precisely the points at which hematopoietic mutants are defective. The development of robust in vitro assays now provide the means to track defined, functional outcomes for prospectively isolated blood cell subsets in the zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping