ZFIN ID: ZDB-PUB-081001-9
The developmental sequence of gene expression within the rod photoreceptor lineage in embryonic zebrafish
Nelson, S.M., Frey, R.A., Wardwell, S.L., and Stenkamp, D.L.
Date: 2008
Source: Developmental dynamics : an official publication of the American Association of Anatomists   237(10): 2903-2917 (Journal)
Registered Authors: Frey, Ruth, Stenkamp, Deborah L.
Keywords: zebrafish, retina, development, rod lineage, retinal homeobox
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Biomarkers
  • Bromodeoxyuridine
  • Cell Lineage*
  • Cell Proliferation
  • Gene Expression Regulation, Developmental/genetics*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Kinetics
  • Retinal Rod Photoreceptor Cells/cytology
  • Retinal Rod Photoreceptor Cells/embryology*
  • Retinal Rod Photoreceptor Cells/metabolism*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 18816851 Full text @ Dev. Dyn.
In postembryonic zebrafish, rod photoreceptors are continuously generated from progenitors in the inner nuclear layer, which are derived from radial Müller glia that express the transcription factor pax6. We used BrdU incorporation, in combination with in situ hybridization for cell-specific transcription factors, to establish the patterns of gene expression during rod lineage maturation in the embryonic zebrafish. Downregulation of pax6 expression was accompanied by sporadic upregulation of expression of the transcription factors NeuroD/nrd, rx1, crx, and Nr2e3/pnr. As cells of the rod lineage entered the outer nuclear layer, they became homogeneous, coordinately expressing NeuroD, rx1, crx, and Nr2e3. Postmitotic, maturing rods also expressed nrl, rod opsin, and rod transducin/gnat1. The presence of rx1 within the rod lineage and in maturing rods indicates that rx1 is not cone-specific, as previously reported, and suggests a high degree of molecular similarity between rod and cone progenitor populations in the zebrafish.