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Research
General Information
ZIRC
ZFIN ID: ZDB-PERS-970127-2
Stenkamp, Deborah L.
Email: dstenkam@uidaho.edu
URL: http://www.uidaho.edu/sci/biology/faculty/deborahstenkamp
Affiliation: Stenkamp Lab
Address: Department of Biological Sciences University of Idaho Moscow, IN 83844-3051 USA
Country: United States
Phone: (208) 885-8963
Fax: (208) 885-7905
Orcid ID:


BIOGRAPHY AND RESEARCH INTERESTS
Dr. Stenkamp's research interests center on the examination of cellular and molecular mechanisms of vertebrate retinal development and regeneration, with a specific focus on photoreceptor differentiation, using zebrafish as the primary experimental model.

One area of current investigation is the involvement of specific factors such as the signaling protein, sonic hedgehog, in regulating the differentiation of rod and cone photoreceptors. The aim is to better define the sources of these factors in the developing retina, and determine their effects on photoreceptors by using gain-of-function and loss-of-function approaches, including the examination of specific zebrafish mutants.

Another area of research is the study of retinal cell patterns in retina that regenerates following a chemical or sugical lesion. A computational approach to describing these patterns has revealed possible distinctions in mechanisms for neurogenesis and cell
differentiation during regeneration as compared to those that operate during normal development and growth. The lab has now begun to carefully compare the processes of ganglion cell regeneration and ganglion cell development using molecular markers.

Collaborators

Dr. David A. Cameron, SUNY Upstate Medical University


PUBLICATIONS
McGinn, T.E., Galicia, C.A., Leoni, D.C., Partington, N., Mitchell, D.M., Stenkamp, D.L. (2019) Rewiring the Regenerated Zebrafish Retina: Reemergence of Bipolar Neurons and Cone-Bipolar Circuitry Following an Inner Retinal Lesion. Frontiers in cell and developmental biology. 7:95
Mitchell, D.M., Sun, C., Hunter, S.S., New, D.D., Stenkamp, D.L. (2019) Regeneration associated transcriptional signature of retinal microglia and macrophages. Scientific Reports. 9:4768
Sun, C., Mitchell, D.M., Stenkamp, D.L. (2018) Isolation of photoreceptors from mature, developing, and regenerated zebrafish retinas, and of microglia/macrophages from regenerating zebrafish retinas. Experimental Eye Research. 177:130-144
Galicia, C.A., Sukeena, J.M., Stenkamp, D.L., Fuerst, P.G. (2018) Expression patterns of dscam and sdk gene paralogs in developing zebrafish retina.. Molecular Vision. 24:443-458
Sun, C., Galicia, C., Stenkamp, D.L. (2018) Transcripts within rod photoreceptors of the Zebrafish retina. BMC Genomics. 19:127
Patel, J.S., Brown, C.J., Ytreberg, F.M., Stenkamp, D.L. (2018) Predicting peak spectral sensitivities of vertebrate cone visual pigments using atomistic molecular simulations. PLoS Computational Biology. 14:e1005974
McGinn, T.E., Mitchell, D.M., Meighan, P.C., Partington, N., Leoni, D.C., Jenkins, C.E., Varnum, M.D., Stenkamp, D.L. (2017) Restoration of Dendritic Complexity, Functional Connectivity, and Diversity of Regenerated Retinal Bipolar Neurons in Adult Zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 38(1):120-136
Sukeena, J.M., Galicia, C.A., Wilson, J.D., McGinn, T., Boughman, J.W., Robison, B.D., Postlethwait, J.H., Braasch, I., Stenkamp, D.L., Fuerst, P.G. (2016) Characterization and Evolution of the Spotted Gar Retina. Journal of experimental zoology. Part B, Molecular and developmental evolution. 326(7):403-421
Mitchell, D.M., Stevens, C.B., Frey, R.A., Hunter, S.S., Ashino, R., Kawamura, S., Stenkamp, D.L. (2015) Retinoic Acid Signaling Regulates Differential Expression of the Tandemly-Duplicated Long Wavelength-Sensitive Cone Opsin Genes in Zebrafish. PLoS Genetics. 11:e1005483
Stenkamp, D.L. (2015) Chapter 23: Development of the Vertebrate Eye and Retina. Progress in molecular biology and translational science. 134:397-414
Sherpa, T., Lankford, T., McGinn, T.E., Hunter, S.S., Frey, R.A., Sun, C., Ryan, M., Robison, B.D., and Stenkamp, D.L. (2014) Retinal regeneration is facilitated by the presence of surviving neurons. Developmental Neurobiology. 74(9):851-76
Kashyap, B., Pegorsch, L., Frey, R.A., Sun, C., Shelden, E.A., and Stenkamp, D.L. (2014) Eye-specific gene expression following embryonic ethanol exposure in zebrafish: Roles for heat shock factor 1. Reproductive toxicology (Elmsford, N.Y.). 43:111-124
Sherpa, T., Hunter, S.S., Frey, R.A., Robison, B.D., and Stenkamp, D.L. (2011) Retinal proliferation response in the buphthalmic zebrafish, bugeye. Experimental Eye Research. 93(4):424-36
Kashyap, B., Frey, R.A., and Stenkamp, D.L. (2011) Ethanol-Induced Microphthalmia is Not Mediated by Changes in Retinoic Acid or Sonic Hedgehog Signaling During Retinal Neurogenesis. Alcoholism, clinical and experimental research. 35(9):1644-61
Stevens, C.B., Cameron, D.A., and Stenkamp, D.L. (2011) Plasticity of photoreceptor-generating retinal progenitors revealed by prolonged retinoic acid exposure. BMC Developmental Biology. 11(1):51
Nelson, S.M., Mahmoud, T., Beaux, M. 2nd, Shapiro, P., McIlroy, D.N., and Stenkamp, D.L. (2010) Toxic and Teratogenic Silica Nanowires in Developing Vertebrate Embryos. Nanomedicine : nanotechnology, biology, and medicine. 6(1):e93-e102
Nelson, S.M., Park, L., and Stenkamp, D.L. (2009) Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish. Developmental Biology. 328(1):24-39
Stenkamp, D.L., Satterfield, R., Muhunthan, K., Sherpa, T., Vihtelic, T.S., and Cameron, D. (2008) Age-Related Cone Abnormalities in Zebrafish with Genetic Lesions in Sonic Hedgehog. Investigative ophthalmology & visual science. 49(10):4631-4640
Nelson, S.M., Frey, R.A., Wardwell, S.L., and Stenkamp, D.L. (2008) The developmental sequence of gene expression within the rod photoreceptor lineage in embryonic zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 237(10):2903-2917
Stenkamp, D.L. (2008) Multiple Roles for Hedgehog Signalling in Zebrafish Eye Development. Molecular Biology Intelligence Unit. :56-68
Sherpa, T., Fimbel, S.M., Mallory, D.E., Maaswinkel, H., Spritzer, S.D., Sand, J.A., Li, L., Hyde, D.R., and Stenkamp, D.L. (2008) Ganglion cell regeneration following whole-retina destruction in zebrafish. Developmental Neurobiology. 68(2):166-181
Kashyap, B., Frederickson, L.C., and Stenkamp, D.L. (2007) Mechanisms for persistent microphthalmia following ethanol exposure during retinal neurogenesis in zebrafish embryos. Visual neuroscience. 24(3):409-421
Helterline, D.L., Garikipati, D., Stenkamp, D.L., and Rodgers, B.D. (2007) Embryonic and tissue-specific regulation of myostatin-1 and -2 gene expression in zebrafish. General and comparative endocrinology. 151(1):90-97
Stenkamp, D.L. (2007) Neurogenesis in the fish retina. International review of cytology. 259(1):173-224
Nickerson, J.M., Frey, R.A., Ciavatta, V.T., and Stenkamp, D.L. (2006) Interphotoreceptor retinoid-binding protein gene structure in tetrapods and teleost fish. Molecular Vision. 12(1):1565-1585
Prabhudesai, S.N., Cameron, D.A., and Stenkamp, D.L. (2005) Targeted effects of retinoic acid signaling upon photoreceptor development in zebrafish. Developmental Biology. 287(1):157-167
Stenkamp, D.L. and Frey, R.A. (2003) Extraretinal and retinal hedgehog signaling sequentially regulate retinal differentiation in zebrafish. Developmental Biology. 258(2):349-363
Stenkamp, D.L., Frey, R.A., Mallory, D.E., and Shupe, E.E. (2002) Embryonic retinal gene expression in sonic-you mutant zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 225(3):344-350
Stenkamp, D.L. and Frey, R.A. (2001) The eye phenotype of zebrafish lacking the sonic hedgehog gene. Investigative ophthalmology & visual science. 42(4):3936
Stenkamp, D.L., Frey, R.A., Prabhudesai, S.N., and Raymond, P.A. (2000) Function for hedgehog genes in zebrafish retinal development. Developmental Biology. 220(2):238-252
Stenkamp D.L., Cunningham, L.L., Raymond, P.A., and Gonzalez-Fernandez, F. (1998) Novel expression pattern of interphotoreceptor retinoid-binding protein (IRBP) in the adult and developing zebrafish retina and RPE. Molecular Vision. 4:26
Rajendran, R.R., Van, Niel, E.E., Stenkamp, D.L., Cunningham, L.L., Raymond, P.A., and Gonzalez-Fernandez, F. (1996) Zebrafish interphotoreceptor retinoid-binding protein: differential circadian expression among cone subtypes. The Journal of experimental biology. 199(12):2775-2787
Raymond, P.A., Barthel, L.K., and Stenkamp, D.L. (1996) The zebrafish ultraviolet cone opsin reported previously is expressed in rods. Investigative ophthalmology & visual science. 37(5):948-950

NON-ZEBRAFISH PUBLICATIONS
Wan, J. and D.L. Stenkamp (2000). Cone mosaic development in the goldfish retina is independent of rod neurogenesis and differentiation. J. Comp. Neurol. 423:227-242.

Stenkamp, D.L., L.K. Barthel and P.A. Raymond (1997). Spatiotemporal coordination of rod and cone differentiation in goldfish retina. J. Comp. Neurol. 382:272-284.

Stenkamp, D.L., O. Hisatomi, L.K. Barthel, F. Tokunaga and P.A. Raymond (1996). Temporal expression of rod and cone opsins in embryonic goldfish retina predicts the spatial organization of the cone mosaic. Inves. Ophthalmol. Vis. Sci. 37:363-376.