A transgenic zebrafish model of neutrophilic inflammation
- Renshaw, S.A., Loynes, C.A., Trushell, D.M., Elworthy, S., Ingham, P.W., and Whyte, M.K.
- Blood 108(13): 3976-3978 (Journal)
- Registered Authors
- Elworthy, Stone, Ingham, Philip, Renshaw, Steve A.
- MeSH Terms
- Animals, Genetically Modified
- Cell Count/methods
- Disease Models, Animal
- Green Fluorescent Proteins
- Image Processing, Computer-Assisted
- Promoter Regions, Genetic
- Wounds and Injuries/genetics
- Wounds and Injuries/immunology*
- Wounds and Injuries/pathology
- 16926288 Full text @ Blood
Renshaw, S.A., Loynes, C.A., Trushell, D.M., Elworthy, S., Ingham, P.W., and Whyte, M.K. (2006) A transgenic zebrafish model of neutrophilic inflammation. Blood. 108(13):3976-3978.
We have established an in vivo model for the genetic analysis of the inflammatory response by generating a transgenic zebrafish line which expresses GFP under the neutrophil-specific myeloperoxidase promoter. We show that inflammation is induced following transection of the tail of zebrafish larvae, and that this inflammation subsequently resolves over a similar timecourse to mammalian systems. Quantitative data can be generated from this model, by counting of fluorescent cells or by digital image analysis. In addition, we show that the resolution of experimentally-induced inflammation can be inhibited by the addition of a pan-caspase inhibitor, zVD.fmk, demonstrating that experimental manipulation of the resolution of inflammation is possible in this model.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes