ZFIN ID: ZDB-PUB-060825-19
A transgenic zebrafish model of neutrophilic inflammation
Renshaw, S.A., Loynes, C.A., Trushell, D.M., Elworthy, S., Ingham, P.W., and Whyte, M.K.
Date: 2006
Source: Blood   108(13): 3976-3978 (Journal)
Registered Authors: Elworthy, Stone, Ingham, Philip, Renshaw, Steve A.
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Count/methods
  • Disease Models, Animal
  • Green Fluorescent Proteins
  • Image Processing, Computer-Assisted
  • Inflammation/genetics
  • Inflammation/immunology
  • Inflammation/pathology
  • Neutrophils/immunology*
  • Neutrophils/pathology
  • Peroxidase/genetics
  • Peroxidase/immunology
  • Promoter Regions, Genetic
  • Transgenes/immunology
  • Wounds and Injuries/genetics
  • Wounds and Injuries/immunology*
  • Wounds and Injuries/pathology
  • Zebrafish/genetics
  • Zebrafish/immunology*
PubMed: 16926288 Full text @ Blood
We have established an in vivo model for the genetic analysis of the inflammatory response by generating a transgenic zebrafish line which expresses GFP under the neutrophil-specific myeloperoxidase promoter. We show that inflammation is induced following transection of the tail of zebrafish larvae, and that this inflammation subsequently resolves over a similar timecourse to mammalian systems. Quantitative data can be generated from this model, by counting of fluorescent cells or by digital image analysis. In addition, we show that the resolution of experimentally-induced inflammation can be inhibited by the addition of a pan-caspase inhibitor, zVD.fmk, demonstrating that experimental manipulation of the resolution of inflammation is possible in this model.