PUBLICATION

A transgenic zebrafish model of neutrophilic inflammation

Authors
Renshaw, S.A., Loynes, C.A., Trushell, D.M., Elworthy, S., Ingham, P.W., and Whyte, M.K.
ID
ZDB-PUB-060825-19
Date
2006
Source
Blood   108(13): 3976-3978 (Journal)
Registered Authors
Elworthy, Stone, Ingham, Philip, Renshaw, Steve A.
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Count/methods
  • Disease Models, Animal
  • Green Fluorescent Proteins
  • Image Processing, Computer-Assisted
  • Inflammation/genetics
  • Inflammation/immunology
  • Inflammation/pathology
  • Neutrophils/immunology*
  • Neutrophils/pathology
  • Peroxidase/genetics
  • Peroxidase/immunology
  • Promoter Regions, Genetic
  • Transgenes/immunology
  • Wounds and Injuries/genetics
  • Wounds and Injuries/immunology*
  • Wounds and Injuries/pathology
  • Zebrafish/genetics
  • Zebrafish/immunology*
PubMed
16926288 Full text @ Blood
Abstract
We have established an in vivo model for the genetic analysis of the inflammatory response by generating a transgenic zebrafish line which expresses GFP under the neutrophil-specific myeloperoxidase promoter. We show that inflammation is induced following transection of the tail of zebrafish larvae, and that this inflammation subsequently resolves over a similar timecourse to mammalian systems. Quantitative data can be generated from this model, by counting of fluorescent cells or by digital image analysis. In addition, we show that the resolution of experimentally-induced inflammation can be inhibited by the addition of a pan-caspase inhibitor, zVD.fmk, demonstrating that experimental manipulation of the resolution of inflammation is possible in this model.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes