|ZFIN ID: ZDB-PUB-060302-6|
Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs
Giraldez, A.J., Mishima, Y., Rihel, J., Grocock, R.J., Van Dongen, S., Inoue, K., Enright, A.J., and Schier, A.F.
|Source:||Science (New York, N.Y.) 312(5770): 75-79 (Journal)|
|Registered Authors:||Giraldez, Antonio, Inoue, Kunio, Mishima, Yuichiro, Rihel, Jason, Schier, Alexander|
|PubMed:||16484454 Full text @ Science|
Giraldez, A.J., Mishima, Y., Rihel, J., Grocock, R.J., Van Dongen, S., Inoue, K., Enright, A.J., and Schier, A.F. (2006) Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs. Science (New York, N.Y.). 312(5770):75-79.
ABSTRACTMicroRNAs comprise 1-3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. Using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target mRNAs. Most targets are maternally expressed mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis.