ZFIN ID: ZDB-PUB-030721-4
The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fish
Ward, A.C., McPhee, D.O., Condron, M.M., Varma, S., Cody, S.H., Onnebo, S.M., Paw, B.H., Zon, L.I., and Lieschke, G.J.
Date: 2003
Source: Blood   102(9): 3238-3240 (Journal)
Registered Authors: Lieschke, Graham J., McPhee, Dora, Paw, Barry, Varma, Sony, Ward, Alister C., Zon, Leonard I.
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • DNA, Complementary
  • Embryo, Nonmammalian
  • Green Fluorescent Proteins
  • Luminescent Proteins/genetics
  • Myeloid Cells/metabolism*
  • Promoter Regions, Genetic/genetics*
  • Proto-Oncogene Proteins/genetics*
  • Trans-Activators/genetics*
  • Transgenes
  • Zebrafish/genetics*
PubMed: 12869502 Full text @ Blood
The spi1 (pu.1) gene has recently been identified as a useful marker of early myeloid cells in zebrafish. In order to enhance the versatility of this organism as a model for studying myeloid development, the promoter of this gene has been isolated and characterized. Transient transgenesis revealed that a 5.3 kb promoter fragment immediately upstream of the spi1 coding sequence was sufficient to drive expression of EGFP in injected embryos in a manner that largely recapitulated the native spi1 gene expression pattern. This fragment was successfully used to produce a germline transgenic line of zebrafish with EGFP-expressing myeloid cells. These TG(spi1:EGFP)(pA301) transgenic zebrafish represent a valuable tool for further studies of myeloid development and its perturbation.