PUBLICATION
Regulation of nodal signalling and mesendoderm formation by TARAM-A, a TGFbeta-related type I receptor
- Authors
- Aoki, T.O., Mathieu, J., Saint-Etienne, L., Rebagliati, M.R., Peyriéras, N., and Rosa, F.M.
- ID
- ZDB-PUB-020115-3
- Date
- 2002
- Source
- Developmental Biology 241(2): 273-288 (Journal)
- Registered Authors
- Mathieu, Juliette, Rebagliati, Michael, Rosa, Frederic, Saint-Etienne, Laure
- Keywords
- zebrafish; nodal; endoderm; signalling; dominant-negative
- MeSH Terms
-
- Biomarkers
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/physiology*
- Endoderm/physiology*
- Mesoderm/physiology*
- Homeodomain Proteins/genetics
- Homeodomain Proteins/physiology
- Intracellular Signaling Peptides and Proteins
- Recombinant Fusion Proteins/physiology
- Phenotype
- Signal Transduction/genetics
- Signal Transduction/physiology*
- Reverse Transcriptase Polymerase Chain Reaction
- Gene Expression Regulation, Developmental*
- Embryo, Nonmammalian/physiology
- Zebrafish/embryology
- Zebrafish/genetics
- Protein Serine-Threonine Kinases*
- Receptors, Transforming Growth Factor beta/genetics
- Receptors, Transforming Growth Factor beta/physiology*
- Bone Morphogenetic Proteins/physiology
- Nodal Signaling Ligands
- Mutagenesis
- Genes, Dominant
- Body Patterning/genetics
- Body Patterning/physiology*
- Activins/metabolism
- Zebrafish Proteins*
- Nodal Protein
- Animals
- Transcription Factors/genetics
- Transcription Factors/physiology
- PubMed
- 11784111 Full text @ Dev. Biol.
Citation
Aoki, T.O., Mathieu, J., Saint-Etienne, L., Rebagliati, M.R., Peyriéras, N., and Rosa, F.M. (2002) Regulation of nodal signalling and mesendoderm formation by TARAM-A, a TGFbeta-related type I receptor. Developmental Biology. 241(2):273-288.
Abstract
Nodal signalling is essential for many developmental events during vertebrate development, including the establishment of left-right asymmetry, of dorsoventral axis of the central nervous system, and endoderm and mesoderm formation. The zebrafish TGFbeta-related type I receptor, TARAM-A (Tar), is expressed in the prospective mesendodermal territory and, when activated, can transfate early blastomeres into endoderm, suggesting that Nodal and Tar may represent similar signalling pathways. We have analysed the functional relationships between those two pathways in zebrafish. We first demonstrate that tar and the zebrafish nodal genes cyc and sqt functionally interact. We also show that a dominant-negative isoform of Tar, TarMR, interferes specifically with the function of Cyc and Sqt in vitro, but does not interfere with the function of BMP2, another TGFbeta-related molecule. TarMR interferes also with Nodal signalling in vivo since it enhances the phenotyp! e of embryos with weakened Nodal signalling. Overexpression of tarMR in wild-type embryos interfered with the formation of endoderm-derived structures. Conversely, overexpression of tar enlarged the presumptive mesendodermal region at the onset of gastrulation. Together, our results point to Tar as an essential factor for endoderm formation and an important modulator of Nodal signalling, potentially representing one of the Nodal receptors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping