ZFIN ID: ZDB-PUB-010927-3
Zebrafish smoothened functions in ventral neural tube specification and axon tract formation
Varga, Z.M., Amores, A., Lewis, K.E., Yan, Y.-L., Postlethwait, J.H., Eisen, J.S., and Westerfield, M.
Date: 2001
Source: Development (Cambridge, England) 128(18): 3497-3509 (Journal)
Registered Authors: Amores, Angel, Eisen, Judith S., Lewis, Katharine E., Postlethwait, John H., Varga, Zoltan M., Westerfield, Monte, Yan, Yi-Lin
Keywords: hedgehog; forebrain patterning; spinal cord; floor plate; motoneurons; pituitary ventral neural tube; zebrafish
MeSH Terms: Animals; Body Patterning*; Hedgehog Proteins; Molecular Sequence Data; Motor Neurons (all 27) expand
PubMed: 11566855
FIGURES   (current status)
ABSTRACT
Sonic hedgehog (Shh) signaling patterns many vertebrate tissues. shh mutations dramatically affect mouse ventral forebrain and floor plate but produce minor defects in zebrafish. Zebrafish have two mammalian Shh orthologs, sonic hedgehog and tiggy-winkle hedgehog, and another gene, echidna hedgehog, that could have overlapping functions. To examine the role of Hedgehog signaling in zebrafish, we have characterized slow muscle omitted (smu) mutants. We show that smu encodes a zebrafish ortholog of Smoothened that transduces Hedgehog signals. Zebrafish smoothened is expressed maternally and zygotically and supports specification of motoneurons, pituitary cells and ventral forebrain. We propose that smoothened is required for induction of lateral floor plate and a subpopulation of hypothalamic cells and for maintenance of medial floor plate and hypothalamic cells.
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