ZFIN ID: ZDB-PERS-960805-442
BIOGRAPHY AND RESEARCH INTERESTS
||The Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO
During embryonic development, cells organize themselves into distinct and often complex three-dimensional organs or tissues. In order for proper morphogenesis to occur, a variety of cellular behaviors have to be tightly coordinated (e.g., cell migration, cell-cell adhesion, cell proliferation, cell death, interactions with the environment, changes in cell morphology). Because of this complexity, the molecular and cell biological mechanisms that regulate embryonic morphogenesis remain poorly understood. The central goal of my research is to identify the regulatory mechanisms that orchestrate morphogenetic processes during development and regeneration, and determine how their misregulation can lead to disease.
To dissect complex morphogenetic processes during development and regeneration we identified the zebrafish lateral line as a powerful experimental model. We have been performing genetic screens for mutants with defects in the development of the lateral line and hair cell regeneration and have already isolated approximately 20 mutations. The analysis of some of these mutations has already yielded fundamental insights into mechanisms underlying cell migration and stem cell regulation.
Navajas Acedo, J., Voas, M.G., Alexander, R., Woolley, T., Unruh, J.R., Li, H., Moens, C., Piotrowski, T. (2019) PCP and Wnt pathway components act in parallel during zebrafish mechanosensory hair cell orientation. Nature communications. 10:3993
Nogare, D.D., Nikaido, M., Somers, K., Head, J., Piotrowski, T., Chitnis, A. (2017) In toto imaging of the migrating Zebrafish lateral line primordium at single cell resolution. Developmental Biology. 422(1):14-23
Kozlovskaja-Gumbrienė, A., Yi, R., Alexander, R., Aman, A., Jiskra, R., Nagelberg, D., Knaut, H., McClain, M., Piotrowski, T. (2017) Proliferation-independent regulation of organ size by Fgf/Notch signaling. eLIFE. 6
Jiang, L., Romero-Carvajal, A., Haug, J.S., Seidel, C.W., Piotrowski, T. (2015) Correction to Supporting Information for Jiang et al., Gene-expression analysis of hair cell regeneration in the zebrafish lateral line. Proceedings of the National Academy of Sciences of the United States of America. 112:E6080
Romero-Carvajal, A., Navajas Acedo, J., Jiang, L., Kozlovskaja-Gumbrienė, A., Alexander, R., Li, H., Piotrowski, T. (2015) Regeneration of Sensory Hair Cells Requires Localized Interactions between the Notch and Wnt Pathways. Developmental Cell. 34(3):267-82
Stevenson, T.J., Trinh, T., Kogelschatz, C., Fujimoto, E., Lush, M.E., Piotrowski, T., Brimley, C.J., and Bonkowsky, J.L. (2012) Hypoxia Disruption of Vertebrate CNS Pathfinding through EphrinB2 Is Rescued by Magnesium. PLoS Genetics. 8(4):e1002638
Perlin, J.R., Lush, M.E., Stephens, W.Z., Piotrowski, T., and Talbot, W.S. (2011) Neuronal Neuregulin 1 type III directs Schwann cell migration. Development (Cambridge, England). 138(21):4639-4648
Piotrowski, T., Ahn, D.-G., Schilling, T.F., Nair, S., Ruvinsky, I., Geisler, R., Rauch, G.-J., Haffter, P., Zon, L.I., Zhou, Y., Foott, H., Dawid, I.B., and Ho, R.K. (2003) The zebrafish van gogh mutation disrupts tbx1, which is involved in the DiGeorge deletion syndrome in humans. Development (Cambridge, England). 130(20):5043-5052
Grandel, H., Lun, K., Rauch, G.J., Rhinn, M., Piotrowski, T., Houart, C., Sordino, P., Küchler, A.M., Schulte-Merker, S., Geisler, R., Holder, N., Wilson, S.W., and Brand, M. (2002) Retinoic acid signalling in the zebrafish embryo is necessary during pre-segmentation stages to pattern the anterior-posterior axis of the CNS and to induce a pectoral fin bud. Development (Cambridge, England). 129(12):2851-2865
Schilling, T.F., Piotrowski, T., Grandel, H., Brand, M., Heisenberg, C.P., Jiang, Y.J., Beuchle, D., Hammerschmidt, M., Kane, D.A., Mullins, M.C., van Eeden, F.J., Kelsh, R.N., Furutani-Seiki, M., Granato, M., Haffter, P., Odenthal, J., Warga, R.M., Trowe, T., and Nüsslein-Volhard, C. (1996) Jaw and branchial arch mutants in zebrafish. I. Branchial arches. Development (Cambridge, England). 123:329-344
Piotrowski, T., Schilling, T.F., Brand, M., Jiang, Y.J., Heisenberg, C.P., Beuchle, D., Grandel, H., van Eeden, F.J., Furutani-Seiki, M., Granato, M., Haffter, P., Hammerschmidt, M., Kane, D.A., Kelsh, R.N., Mullins, M.C., Odenthal, J., Warga, R.M., and Nüsslein-Volhard, C. (1996) Jaw and branchial arch mutants in zebrafish. II. Anterior arches and cartilage differentiation. Development (Cambridge, England). 123:345-356