ZFIN Image: Wang et al., 2024, FIGURE 5

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Figure Caption

FIGURE 5

Hypoxia enhances cardiac output in HET(52del) larvae. (A) Representative immunoblots of β‐tubulin and Gapdh in WT, HET(52del), and KO(52del) at normoxic and hypoxic (3% O₂ for 24 h) conditions. Gapdh serves as an internal control. Norm. normoxia; Hypox. Hypoxia. (B) RT‐qPCR analyses indicate that hypoxia induces upregulation of epoa and hmox1a, but not hmox1b, in WT larvae. (C–G) Hypoxia enhances ejection fraction (C) in WT and HET(52del) larvae, and cardiac output (D) and stroke volume (E) in HET(52del) larvae, but not in KO(52del), compared to normoxia. Hypoxia has no effect on heart rate (F) or diastolic area (G) in the three genotypic groups. WT, normoxia n = 15, hypoxia n = 9; HET(52del), normoxia n = 19, hypoxia n = 10; KO(52del), normoxia n = 16, hypoxia n = 10. Data are presented as mean ± SD. Two‐sample t‐test. *p < 0.05, **p < 0.01, ***p < 0.001.

Acknowledgments

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