FIGURE 2

FIGURE 2

Phenotypic screening for hematopoietic‐protective compounds in N,N,N′,N′‐tetrakis(2‐pyridylmethyl)ethylenediamine (TPEN)‐treated zebrafish: (A) construction of an enriched library of natural compounds related to blood diseases; (B) chemical profile of the enriched natural compound library; (C) schematic diagram of phenotypic screening. The number of hematopoietic stem cells (HSCs) (represented by red dots) in the caudal hematopoietic tissue of zebrafish was used as a screening indicator; (D) the scatter plot summarizes the effect of all compounds on the number of HSCs. Efficacy score was calculated by (N_drug−N_model)/(N_control−N_model). The blue dashed line represented the efficacy score of 0.85 and the red dashed line marked the efficacy score of 1.0. Hydroxysafflor yellow A (HSYA) is marked as a green dot and indicated by a black arrow. Compounds that increased the number of HSCs to a higher than normal level are marked as red dots; (E) rescue effects on HSCs with gradient treatment of HSYA in TPEN‐treated zebrafish.