FIGURE 5

FIGURE 5

Effects of hepsin levels on thrombin generation by Caco-2 and Caco-2-HPN cells. Thrombin generation was performed after incubation of plasma with cells for 3 h as described in Materials and Methods. Afterwards, plasma was incubated with PPP reagent ^® (final concentrations: tissue factor, 1 pmol/L; phospholipids, 4 μmol/L) and calcium chloride. The lag time (min) (A), time to peak (min) (B), endogenous thrombin potential (ETP, nmol*min) (C), thrombin peak (peak, nmol) (D), and mean rate index (Velocity, nmol/min) (E) were recorded. The data represent the mean ± standard error of the mean of at least six separate experiments. Caco-2-HPN, Caco-2 cells overexpressing hepsin; Caco-2, Caco-2 cells with hepsin basal expression; *: p-value < 0.05.