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Fig. 6
UPF1 phosphorylation in mouse Rd12 retina/RPE extracts analyzed by western blotting. (A) UPF1 protein expression in wildtype (Wt), untreated Rd12 mutants (Mt), Mt treated postnatally (Mt Post), and Mt treated prenatally plus postnatal treatment (Mt Pre). UPF1 is seen as a doublet on western blotting. (B) Phosphorylated UPF1 (p-UPF1) in Wt, Mt, Mt Post, and Mt Pre protein extracts. (C) β-actin loading control. Protein marker is BLUelf ladder. (D) Quantification of p-UPF1 expression between treatment groups relative to β-actin. Untreated Mt set as 100% p-UPF1expression level.
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