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Fig. 4 Ectopic expression of venous markers in mibta52b mutant embryos. (A) In wild-type siblings flt4 expression is restricted to the PCV by the 30-somite stage. (B) In mibta52b mutant embryos expression persists within both the PCV (blue arrow) and the DA (red arrowhead) at this stage. (C) In wild-type siblings rtk5 is expressed within the hypochord (black arrow), and is detected at a higher level within the PCV (blue arrow) than the DA (red arrowhead). (D) In mibta52b mutant embryos rtk5 expression is absent from the PCV, and regions of ectopic rtk5 expression are noted in the DA (red arrowhead). (E,F) Transmitted light images of (E) wild-type and (F) mibta52b mutant living sibling embryos at 36 hpf. (F) The curled tail and other characteristics (see text) are apparent in the mutant embryo and are similar to embryos injected with EGFPSuDN (see Fig. 2H). Scale bars (A,B) 100 μm, (C,D) 50 μm, (E,F) 200 μm.