ZFIN Image: Sorrell et al., 2013, Fig. 10

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Figure Caption

Fig. 10

Tcf7l1 transcriptional repression is necessary to limit CM progenitors and promote hematovascular progenitor specification. ((A) and (H)) Control embryos. ((B)and (I)) Tcf7l1a deficient embryos. ((C) and (J)) Tcf7l1a deficient embryos co-injected with Δtcf7l1a mRNA. ((D) and (K)) Tcf7l1b deficient embryos. ((E) and (L)) Tcf7l1b deficient embryos co-injected with Δtcf7l1a mRNA. ((F) and (M)) Tcf7l1 deficient embryos. ((G) amd (N)) Tcf7l1 deficient embryos co-injected with Δtcf7l1a mRNA. ((A)–(G)) Tcf7l1a and Tcf7l1 deficient embryos have a decrease in etv2 expression, which is partially restored when Δtcf7l1a mRNA is co-injected. The defect in etv2 expression was less dramatic in Tcf7l1b than the Tcf7l1a and Tcf7l1 deficient embryos. ((H)–(N)) nkx2.5 expression is expanded in Tcf7l1 deficient embryos and partially rescued when Δtcf7l1a mRNA is co-injected. (O) Length of etv2 expression in control sibling and Tcf7l1 deficient embryos. (P) Length of nkx2.5 expression in control sibling and Tcf7l1 deficient embryos. Arrows indicate length of expression. All images are dorsal with anterior up. Asterisk indicates a statistically significant difference compared to controls.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 380(2), Sorrell, M.R., Dohn, T.E., D'Aniello, E., and Waxman, J.S., Tcf7l1 proteins cell autonomously restrict cardiomyocyte and promote endothelial specification in zebrafish, 199-210, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.