ZFIN Image: Yang et al., 2009, Fig. 4

Image

Figure Caption

Fig. 4 brom bones possesses an increased level of Notch signaling activity in the intestinal epithelium.

(A–D) Cross-sections of the anterior intestines from control (A,C) and mutant fish (B,D) immunostained for Hes1 (A,B) and NICD (C,D). Arrowheads in A and B point to Hes1 positive-cells. Arrows in (C) and (D) indicate NICD-positive cells. (E) Quantification of the NICD-positive cell number. Values shown are the percentages of NICD-positive cells relative to the total number of intestinal epithelial cells per villous cross-section (n = 114 villi from 7 brom bones heterozygous fish and 175 villi from 10 brom bones homozygous fish). Error bars represent s.e.m; P = 0.001 by Student's t test. (F,G) Real-time RT-PCR results show the expression levels of her6 (F) and her9 (G) in the intestines of 4 wild-type fish and 4 brom bones homozygotes. The expression levels of her6 and her9 were normalized to odc. Gene expression levels in the wild-type 1 fish were set as 100%. Scale bar = 50 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.