ZFIN Image: Blechman et al., 2007, Fig. S5

Image

Figure Caption

Fig. S5 Effects of otpa antisense RNA probes and otpb mRNA. Wild-type (WT; a) embryos and their too few^m808 (tof^m808) mutant siblings (B) were fixed at 52 hours post fertilization and subjected to whole-mount in situ hybridization with antisense RNA probes directed against otpa followed by immunostaining with an antibody directed against tyrosine hydroxylase (TH). Black arrow heads mark deficient otpa expression domains. (C-F) Embryos were injected with either a buffer solution (Control, C,E) or otpb mRNA (E,F) and were thereafter (48 hours post injection) subjected to either in situ hybridization with probes directed to either pac1 (C,D) or pacap1b (E,F). HB, hindbrain; PT, posterior tuberculum; Tel, telencephalon. Scale bars, 50 μm.

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Expression / Labeling details

Acknowledgments

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