IMAGE

Fig. 8

ID
ZDB-IMAGE-071204-24
Genes
Source
Figures for Li et al., 2007
Image
Figure Caption

Fig. 8 Loss of neural crest in tfap2a/c deficient embryos is concomitant with an expansion of neural plate. (A–D) Lateral views, dorsal to the right, of either uninjected wild-type or tfap2aMO/cMO embryos fixed at 11 hpf and processed to reveal dying cells with TUNEL. A wild-type embryo and a tfap2aMO/cMO embryo have comparable numbers of TUNEL-positive cells [control embryos, 62 ± 70 (average ± standard deviation), n = 13; tfap2aMO/cMO embryos, 42 ± 38, n = 28; unpaired t test, p = 0.23]. (C) A wild-type embryo, and (D) a tfap2aMO/cMO embryo, after treatment with caspase inhibitor zVAD-fmk. Reduced levels of cell death are seen in both of them (control embryos, 10 ± 14, n = 19, p < 0.005 in comparison to untreated controls; tfap2aMO/cMO embryos, 17 ± 13, n = 18, p < 0.05, in comparison to untreated tfap2aMO/cMO embryos). Dorsal views, anterior to the left, of panel E, a wild-type embryo, and panel F, a tfap2aMO/cMO embryo treated with zVAD-fmk and processed to reveal foxd3 expression at 11 hpf. foxd3 expression is absent in the premigratory neural crest (pnc) domain of the zVAD treated tfap2aMO/cMO embryo (30 of 30 zVAD treated tfap2aMO/cMO embryos). (G, H) Ventral views, anterior to the left, of embryos incubated in BrdU for 20 min at 10 hpf, fixed at 11 hpf, and stained for anti-BrdU IR. The number of anti-BrdU IR positive cells within a 200 x 200 μm region of ventral ectoderm (boxes in panels G and H) was comparable in both groups (control embryos, 37 ± 6 anti-BrdU IR positive cells, n = 9 embryos; tfap2aMO/cMO embryos, 35 ± 7 anti-BrdU IR positive cells, n = 9 embryos; unpaired t test, p = 0.48). (I, J) Dorsal views of 11 hpf embryos processed to reveal sox2 expression, labeling neural plate (np), and dlx3b expression, labeling the pre-placodal region (ppr). (I′, J′) Close-up views of right side of embryos shown in panels I and J, respectively. The space between these two expression domains, normally occupied by premigratory neural crest (asterisk in panel I2), is absent in the tfap2aMO/cMO embryo (J, 30 of 32 tfap2aMO/cMO embryos). Scale bars: (A–J), 100 μm; (I′, J′), 50 μm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 304(1), Li, W., and Cornell, R.A., Redundant activities of Tfap2a and Tfap2c are required for neural crest induction and development of other non-neural ectoderm derivatives in zebrafish embryos, 338-354, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.